The anti-ageing potential of rooibos: preserving preadipocyte funtion

Treatments with natural products rich in anti-oxidants have attracted remarkable interest in the cosmetic and pharmaceutical industry to combat oxidative stress and reverse the effects of ageing. Rooibos (Aspalathus linearis) is a South African fynbos plant, well-known for its strong anti-oxidant capacity and use in many cosmetic products. However, little published research exists on its potential as an anti-ageing treatment. The anti-ageing properties of fermented and green rooibos were investigated using an in vitro cell culture model designed to evaluate the involvement of mitochondrial dysfunction in the age related decline in preadipocyte function. Mitochondrial DNA (mtDNA) deficient preadipocytes, ρ0 3T3-L1preadipocytes, were generated following continuous long-term exposure to sub lethal concentrations of ethidium bromide (EtBr). Depletion of the mtDNA resulted in a significantly reduced mitochondrial membrane potential, rate of proliferation in culture, as well as an increased glucose utilization and lactate production. Treatment with the green rooibos (100 μg/mL) stimulated cell growth rates for both the wildtype and mutant cell lines. MtDNA depleted cells showed arrest in the G1 phase (48.8 ± 3.34%) compared to wildtype cells (44.6 ± 1.38%), which was significantly attenuated after treatment with green rooibos for mutant (42.0 ± 0.83%) and wildtype (36.5 ± 5.80%) treated cells. The results obtained for glucose utilization and lactate production, indicated a significant increase in glucose utilization along with a concomitant increase in lactate production after treatment with both green and fermented rooibos for wildtype and mutant cell lines. A significant improvement in mitochondrial membrane potential was also later observed after treatment with green and fermented rooibos on both the wildtype and mutant cell lines. The results obtained indicate that rooibos extracts, particularly the green rooibos, exhibit effects which preserve the functional capacity of preadipocytes exposed to ageing related insults, and indicate that rooibos could cause a metabolic shift in cells redirecting carbon flow away from mitochondrial metabolism, and towards lactate production and consequently, cells become resistant to mitochondrial dysfunction

Anthropometric profile of HIV-uninfected and HIV-infected women aged 25–44 years in Mangaung, Free State

Background: Obesity and human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) affect significant numbers of black women in South Africa.Method: Using township maps, a random sample of 500 black women residing in Mangaung in the Free State was selected to participate in this study in the year 2000. The women were divided into two age groups, namely 25–34 years (n = 273) and 35–44 years (n = 215). Anthropometric measurements, including height, weight [to calculate the body mass index (BMI)] and waist circumference (WC) were taken. Fat percentage was measured with bioelectrical impedance. HIV status was determined using a microparticle enzyme immunoassay method. Socio-demographic status, health status, dietary intake, level of physical activity, body perception and attitude toward weight control, as well as prevalence and risk of lifestylediseases were determined as part of the larger study.Results: Sixty-one per cent of younger women (25–34 years) and 38% of older women (35–44 years) were infected with HIV. In younger HIV-infected women, median BMI, WC and fat percentage were significantly lower than in HIV-uninfected women.Conclusions: HIV infection rates were found to be higher among younger than older women. The prevalence of obesity was high overall. Median BMI values ranged between 24.4 kg/m2 and 27.6 kg/m2. A large percentage of all women fell in the unhealthy fat percentage category (excessive body fat), ranging between 65.9% of HIV-infected young women and 79.3%of older HIV-infected women. HIV, even in the asymptomatic stage, influences anthropometric indicators

The anti-ageing potential of rooibos: preserving preadipocyte funtion

Treatments with natural products rich in anti-oxidants have attracted remarkable interest in the cosmetic and pharmaceutical industry to combat oxidative stress and reverse the effects of ageing. Rooibos (Aspalathus linearis) is a South African fynbos plant, well-known for its strong anti-oxidant capacity and use in many cosmetic products. However, little published research exists on its potential as an anti-ageing treatment. The anti-ageing properties of fermented and green rooibos were investigated using an in vitro cell culture model designed to evaluate the involvement of mitochondrial dysfunction in the age related decline in preadipocyte function. Mitochondrial DNA (mtDNA) deficient preadipocytes, ρ0 3T3-L1preadipocytes, were generated following continuous long-term exposure to sub lethal concentrations of ethidium bromide (EtBr). Depletion of the mtDNA resulted in a significantly reduced mitochondrial membrane potential, rate of proliferation in culture, as well as an increased glucose utilization and lactate production. Treatment with the green rooibos (100 μg/mL) stimulated cell growth rates for both the wildtype and mutant cell lines. MtDNA depleted cells showed arrest in the G1 phase (48.8 ± 3.34%) compared to wildtype cells (44.6 ± 1.38%), which was significantly attenuated after treatment with green rooibos for mutant (42.0 ± 0.83%) and wildtype (36.5 ± 5.80%) treated cells. The results obtained for glucose utilization and lactate production, indicated a significant increase in glucose utilization along with a concomitant increase in lactate production after treatment with both green and fermented rooibos for wildtype and mutant cell lines. A significant improvement in mitochondrial membrane potential was also later observed after treatment with green and fermented rooibos on both the wildtype and mutant cell lines. The results obtained indicate that rooibos extracts, particularly the green rooibos, exhibit effects which preserve the functional capacity of preadipocytes exposed to ageing related insults, and indicate that rooibos could cause a metabolic shift in cells redirecting carbon flow away from mitochondrial metabolism, and towards lactate production and consequently, cells become resistant to mitochondrial dysfunction

The metabolic profiles of HIV-infected and non-infected women in Mangaung, South Africa

Objective: To determine the biochemical nutritional status of HIV-infected women in Mangaung.Design: Cross-sectional.Setting: The community of Mangaung, Free State, South Africa.Subjects: A representative group of 500 black women (25–44 years) was selected randomly to participate.Outcome measures: Biochemical analyses were performed for total  lymphocytes, serum protein, serum albumin, plasma fibrinogen, serum insulin, serum glucose, serum triglycerides and serum cholesterol using standard methodology. Values were compared to standard references, and between HIV-infected and HIV-uninfected women.Results: After screening for eligibility, 488 women qualified. Sixty-one per cent of the younger women (25–34 years) and 38% of the older women (35–44 years) were HIV-infected. HIV-infected women had significantly lower median blood values for total lymphocytes (p = 0.0001 and p = 0.02 for younger and older group respectively) and serum albumin (p = 0.0001 for both age groups), but significantly higher median concentrations of serum protein (p = 0.0001 for both age groups) than uninfected women. Plasma fibrinogen and serum insulin concentrations were significantly lower in HIV-infected younger women than in their uninfected counterparts (p = 0.002 for both parameters). Older HIV-infected women had significantly lower total serum cholesterol values (p = 0.01) than older HIV-uninfected women. Serum glucose and serum triglycerides did not differ significantly between HIV-infected and HIV-uninfected women.Conclusions: The results indicate a possible impact of HIV infection on serum protein and serum albumin, which may adversely affect biochemical nutritional status and the course of HIV progression. Future research into the causes and possible treatment of metabolic changes in women in this community should be prioritised

Assessment of the physical activity, body mass index and energy intake of HIV-uninfected and HIV-infected women in Mangaung, Free State province

Published ArticleDeclining levels of physical activity at workplaces, during leisure time and when travelling, accompanied by increasing exposure to the mass media, are major determinants of the global obesity epidemic. This study aimed to assess physical activity, the body mass index (BMI) and energy intake of human immunodeficiency virus (HIV)-uninfected and HIV-infected black women in Mangaung. Method: A random sample of 500 black women was selected in Mangaung. Physical activity levels, dietary intake and BMI were determined in younger and older women, aged 25–34 and 35–44 years, respectively. Results: Of the 488 women who qualified for participation, 61% of the younger women and 38% of the older women were HIV-infected. Low physical activity levels were reported in most women, and more than 50% were overweight or obese. The BMI of HIV-infected younger women with low physical activity levels (24.9 kg/m2) was significantly lower than that of the HIV-uninfected younger women (27.2 kg/m2) (p-value 0.02). The energy intake of older HIV-infected women with low physical activity levels was significantly lower (10 090 kJ) than that of the older HIV-infected women in the normal to high physical activity category (14 519 kJ) (p-value 0.03). Conclusion: A more active lifestyle and energy-reduced diet that focuses on food quality could partially address BMI parameters in HIV-uninfected women. Safeguarding a lean BMI in HIV-infected women, by increasing physical activity levels while maintaining current energy intake, with an emphasis on healthy eating practices, could support quality of life

The relationship between body mass index, energy intake and level of physical activity of HIV positive women (25-44 years) in Mangaung

Published ArticleObjective: To determine the relationship between body mass index (BMI), energy intake and levels of physical activity of HIV negative and HIV positive women. Design: Cross sectional study. Methodology: A random sample of 500 pre-menopausal women within the two age group categories of 25-34 and 35-44 years was selected. Data on physical activity was obtained using an adapted Baecke questionnaire and categorized into low, medium, and high levels of physical activity. Weight and height were used to calculate BMI. Dietary intake was determined by means of a standardized food frequency questionnaire. Results: Sixty-one percent of younger women and 38% of older women were HIV infected. The vast majority of women (91%) had low levels of physical activity, while only 9% of the sample had physical activity levels that fell within the normal to high category. More than 50% of respondents were either overweight or obese (BMI above 25 kg/m2). BMI of HIV positive younger women was, however, significantly lower than that of HIV negative women. Median energy intakes were high (more than 10 000 kJ) for both HIV positive and HIV negative women. Conclusions: Reverting to a more traditional lifestyle, including diet and physical activity, could assist in addressing unfavorable BMI parameters of these women and improve health status and quality of life of HIV infected women

Community pharmacists' opinions of their role in administering non-prescription medicines in an emergency

Objective: To obtain community pharmacists’ opinions of their role in administering Pharmacy (S2) and Pharmacist Only (S3) Medicines in a medical emergency. These medicines can only be sold in a pharmacy and are not available for self-selection by patients. Whilst qualified pharmacy assistants can supply S2 medicines, pharmacists must be directly involved in the supply of S3 medicines. Setting: Community pharmacies in South East Queensland, Australia. Method: A survey of 151 Gold Coast and Toowoomba community pharmacists was conducted during October 2009. Main outcome measures: Pharmacists were asked their opinions as to whether the administration of S2 and S3 medicines should fall within their scope of practice, whether they had administered S2 and S3 medicines in a medical emergency in the past and if clarification of this role was required. Results: The study achieved a 30% (n = 45) response rate and demonstrated similar results regarding whether pharmacists should administer salbutamol (22/44), adrenaline (23/42), glyceryl trinitrate (22/43) and aspirin (18/36) in a medical emergency. The majority (36/43) believed that role clarification was required. Pharmacists were more likely to administer an S3 medicine in a medical emergency when they considered potential outcomes first, had no easy access to a doctor and the patient could not administer the medicine they carried with them themselves (40/45).Conclusion: Community pharmacists have direct access to S2 and S3 medicines that could be required in the management of a variety of medical emergencies. This study demonstrates that some pharmacists have administered S2 and S3 medicines in an emergency situation. However, there are currently no clear guidelines for pharmacists when faced with a medical emergency other than to act within their professional competence. To promote patient safety through the appropriate use of S2 and S3 medicines in the event of a medical emergency, additional training of pharmacists on the administration of these readily accessible medicines is needed. Clarification of the role of pharmacists in an emergency situation is required

Possible Pleistocene hominin tracks from South Africa’s west coast

Two probable tracks have been identified on the ceiling of a small overhang in the Pleistocene Langebaan Formation on South Africa’s west coast. They may have been made by a hominin trackmaker. They appear to have been registered at walking speed on a level, sandy dune substrate. Three tracks, attributed to Homo sapiens, were previously identified near Langebaan in 1995, and were popularly labelled ‘Eve’s Footprints’. The new identification of possible hominin tracks near Langebaan is the second from South Africa’s west coast. This discovery adds to the sparse but growing global record of possible hominin tracks preserved in aeolianites. Significance:• Two probable fossil tracks have been identified on the ceiling of an overhang near Langebaan.• The tracks may have been made by a human walking on a level dune surface during the Pleistocene.• This discovery is the second of its kind on the west coast, and complements the 1995 identification near Langebaan of Pleistocene fossil tracks attributed to humans

Inhibition of Brevibacterium linens by Probiotics from Dairy Products

Brevibacterium linens is an important species in dairy products rendering a specific taste and aroma to numerous smear ripened and blue veined cheeses due to proteolysis. However, the presence of the species in South African blue veined cheeses is undesirable and consumers demand the product void of the species. Accordingly, numerous methods including microbial inhibition using fungi and bacterial probiotic cultures with possible inhibitory effects were applied in an attempt to inhibit the species. None of the fungi, however, proved to be successful, whereas Lactobacillus rhamnosus and Bifidobacterium lactis, two typical probiotic species applied in dairy products, showed inhibitory effects against B. linens when tested using the spot-on-lawn assay