The influence of nitrification in determining the supply, distribution and fate of nitrogen in grassland soils

Merged with duplicate record 10026.1/815 on 15.02.2017 by CS (TIS)The microbiology of nitrification has been extensively studied, but the ecology and environmental impact of the process has received less attention. The reason for this has more to do with the difficulties of conducting field experiments to examine the links with other processes which cause losses of nitrogen, than a failure to appreciate its importance in agricultural systems. This project was designed to overcome some of the limitations of existing field techniques to enable simultaneous measurements of nitrification and the major processes of N supply (mineralization) and N loss (denitrification and leaching) to be examined. The study proceeded in three distinct phases: firstly, soils with contrasting N management histories were examined, using laboratory assays for potential activities. Clear differences which resulted from higher N inputs were established, with correspondingly higher nitrifying activities. For example, in a fertilized soil, ammonia-oxidizers produced 48.4 compared with 1.3 nM N02 gˉ¹ soil hˉ¹ in an unfertilized soil. Potential nitrite-oxidizing rates were 93.4 and 62.5 nM gˉ¹ hˉ¹ respectively. Assays of enzyme kinetics, therefore confirmed the higher nitrifying activity in the fertilized soil, but demonstrated a lower affinity of the enzyme for N02- substrate, with K, values of 436 and 310 µM N02 -N, respectively. Nitrifying rates in soils from grass-clover swards were intermediate between the fertilized and unfertilized soils. Secondly, a new field incubation technique was developed and used to obtain actual rates by concurrent measurements of the major N cycling processes. A strong correlation was established between nitrification and denitrification (r² = 0. 98). The measurements were verified by comparison with other independent methods. Net rates of nitrification in the same soil type ranged from 0.55 - 1.17 kg N haˉ¹ dˉ¹ , with the highest rates in the fertilized soil. Over 70% of the mineralized N was nitrified, of which 80% was subsequently lost (i.e. either denitrified or leached). Thirdly, the practical implications of these findings were examined in greater detail using 15N labelling techniques which enabled process rates (net and gross) to be established in a model of the N cycle. When nitrification was inhibited, there were no significant differences between gross or net mineralization rates in the soils from the three swards, which indicated that N-immobilization could be directly influenced by the level of nitrifying activity in these soils. The influence of nitrification in determining the pathways of N loss from grassland soils was quantified in this study. From a detailed investigation of the processes involved in N cycling, it was deduced that nitrification was also one of the major factors in determining the outcome of competition for inorganic N between plant and microbial biomasses.The Institute of Grassland and Environmental Researc

The Critical Role of Public Charging Infrastructure

Editors: Peter Fox-Penner, PhD, Z. Justin Ren, PhD, David O. JermainA decade after the launch of the contemporary global electric vehicle (EV) market, most cities face a major challenge preparing for rising EV demand. Some cities, and the leaders who shape them, are meeting and even leading demand for EV infrastructure. This book aggregates deep, groundbreaking research in the areas of urban EV deployment for city managers, private developers, urban planners, and utilities who want to understand and lead change

Signs of environmental effects on star-forming galaxies in the Spiderweb protocluster at z=2.16

We use multi-object near-infrared (NIR) spectroscopy with VLT/KMOS to investigate the role of the environment in the evolution of the ionized gas properties of narrow-band selected H$\alpha$ emitters (HAEs) in the Spiderweb protocluster at $z=2.16$. Based on rest-frame optical emission lines, H$\alpha$ and [NII]$\lambda$6584, we confirm the cluster membership of 39 of our targets (i.e. 93% success rate), and measure their star-formation rates (SFR), gas-phase oxygen abundances and effective radius. We parametrize the environment where our targets reside by using local and global density indicators based on previous samples of spectroscopic and narrow-band cluster members. We find that star-forming galaxies embedded in the Spiderweb protocluster display SFRs compatible with those of the main sequence and morphologies comparable to those of late-type galaxies at $z=2.2$ in the field. We also report a mild gas-phase metallicity enhancement ($0.6\pm0.3$ dex) at intermediate stellar masses. Furthermore, we identify two UVJ-selected quiescent galaxies with residual H$\alpha$-based star formation and find signs of extreme dust obscuration in a small sample of SMGs based on their FIR and H$\alpha$ emission. Interestingly, the spatial distribution of these objects differs from the rest of HAEs, avoiding the protocluster core. Finally, we explore the gas fraction-gas metallicity diagram for 7 galaxies with molecular gas masses measured by ATCA using CO(1-0). In the context of the gas-regulator model, our objects are consistent with relatively low mass-loading factors, suggesting lower outflow activity than field samples at the cosmic noon and thus, hinting at the onset of environmental effects in this massive protocluster.Comment: 29 pages, 15 figures. Accepted for publication in MNRA

Progress in Assessing Air Pollutant Risks from In Vitro Exposures: Matching Ozone Dose and Effect in Human Airway Cells

In vitro exposures to air pollutants could, in theory, facilitate a rapid and detailed assessment of molecular mechanisms of toxicity. However, it is difficult to ensure that the dose of a gaseous pollutant to cells in tissue culture is similar to that of the same cells during in vivo exposure of a living person. The goal of the present study was to compare the dose and effect of O3 in airway cells of humans exposed in vivo to that of human cells exposed in vitro. Ten subjects breathed labeled O3 (18O3, 0.3 ppm, 2 h) while exercising intermittently. Bronchial brush biopsies and lung lavage fluids were collected 1 h post exposure for in vivo data whereas in vitro data were obtained from primary cultures of human bronchial epithelial cells exposed to 0.25–1.0 ppm 18O3 for 2 h. The O3 dose to the cells was defined as the level of 18O incorporation and the O3 effect as the fold increase in expression of inflammatory marker genes (IL-8 and COX-2). Dose and effect in cells removed from in vivo exposed subjects were lower than in cells exposed to the same 18O3 concentration in vitro suggesting upper airway O3 scrubbing in vivo. Cells collected by lavage as well as previous studies in monkeys show that cells deeper in the lung receive a higher O3 dose than cells in the bronchus. We conclude that the methods used herein show promise for replicating and comparing the in vivo dose and effect of O3 in an in vitro system

The Ginninderra CH4 and CO2 release experiment: An evaluation of gas detection and quantification techniques

A methane (CH4) and carbon dioxide (CO2) release experiment was held from April to June 2015 at the Ginninderra Controlled Release Facility in Canberra, Australia. The experiment provided an opportunity to compare different emission quantification techniques against a simulated CH4 and CO2 point source release, where the actual release rates were unknown to the participants. Eight quantification techniques were assessed: three tracer ratio techniques (two mobile); backwards Lagrangian stochastic modelling; forwards Lagrangian stochastic modelling; Lagrangian stochastic (LS) footprint modelling; atmospheric tomography using point and using integrated line sensors. The majority of CH4 estimates were within 20% of the actual CH4 release rate (5.8 g/min), with the tracer ratio technique providing the closest estimate to both the CH4 and CO2 release rates (100 g/min). Once the release rate was known, the majority of revised estimates were within 10% of the actual release rate. The study illustrates the power of measuring the emission rate using multiple simultaneous methods and obtaining an ensemble median or mean. An ensemble approach to estimating the CH4 emission rate proved successful with the ensemble median estimate within 16% for the actual release rate for the blind release experiment and within 2% once the release rate was known. The release also provided an opportunity to assess the effectiveness of stationary and mobile ground and aerial CH4 detection technologies. Sensor detection limits and sampling rates were found to be significant limitations for CH4 and CO2 detection. A hyperspectral imager\u27s capacity to image the CH4 release from 100 m, and a Boreal CH4 laser sensor\u27s ability to track moving targets suggest the future possibility to map gas plumes using a single laser and mobile aerial reflector