Doppler Tomography of Dwarf Nova IY UMa during Quiescence

Quiescent Doppler tomography of the newly discovered deeply-eclipsing SU UMa system IY UMa reveals properties of the region where the accretion stream from the donor impacts the edge of the disc. A very strong bright spot is produced and the Keplerian disc emission in the impact region is disrupted or obscured. The differing properties of Halpha, Hbeta and He I emission will allow physical parameters of the converging flow region to be studied.Comment: 6 pages, 5 figures. To appear in Proceedings of Astro-Tomography Workshop, Brussels, July 2000, Eds. H. Boffin, D. Steeghs, Springer-Verlag Lecture Notes in Physic

MscS-like Proteins Control Plastid Size and Shape in Arabidopsis thaliana

Background Mechanosensitive (MS) ion channels provide a mechanism for the perception of mechanical stimuli such as sound, touch, and osmotic pressure. The bacterial MS ion channel MscS opens in response to increased membrane tension and serves to protect against cellular lysis during osmotic downshock. MscS-like proteins are found widely in bacterial and archaeal species and have also been identified in fission yeast and plants. None of the eukaryotic members of the family have yet been characterized. Results Here, we characterize two MscS-like (MSL) proteins from Arabidopsis thaliana, MSL2 and MSL3. MSL3 can rescue the osmotic-shock sensitivity of a bacterial mutant lacking MS-ion-channel activity, suggesting that it functions as a mechanosensitive ion channel. Arabidopsis plants harboring insertional mutations in both MSL3 and MSL2 show abnormalities in the size and shape of plastids, which are plant-specific endosymbiotic organelles responsible for photosynthesis, gravity perception, and numerous metabolic reactions. MSL2-GFP and MSL3-GFP are localized to discrete foci on the plastid envelope and colocalize with the plastid division protein AtMinE. Conclusions Our data support a model wherein MSL2 and MSL3 control plastid size, shape, and perhaps division during normal plant development by altering ion flux in response to changes in membrane tension. We propose that MscS family members have evolved new roles in plants since the endosymbiotic event that gave rise to plastids

Hydrodynamic modelling of accretion flows

In the proceedings of this, and of several recent close binary conferences, there have been several contributions describing smoothed particle hydrodynamics simulations of accretion disks. It is apposite therefore to review the numerical scheme itself with emphasis on its advantages for disk modelling, and the methods used for modelling viscous processes.Comment: 3 pages, to appear in proceedings of IAU Colloquium 194: Compact binaries in the galaxy and beyon

Plastids and pathogens: Mechanosensitive channels and survival in a hypoosmotic world

In bacteria, MscS-type mechanosensitive channels serve to protect cells from lysis as they swell during extreme osmotic stress. We recently showed that two MscS homologs from Arabidopsis thaliana serve a similar purpose in the epidermal plastids of the leaf, indicating that the plant cell cytoplasm can present a dynamic osmotic challenge to the plastid. MscS homologs are predicted to be targeted to both plastids and mitochondrial envelopes and have been found in the genomes of intracellular pathogens. Here we discuss the implications of these observations, and propose that MS channels provide an essential mechanism for osmotic adaptation to both intracellular and the extracellular environments

Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1

Mechanosensitive (MS) ion channels are widespread mechanisms for cellular mechanosensation that can be directly activated by increasing membrane tension. The well-studied MscS family of MS ion channels is found in bacteria, archaea, and plants. MscS-Like (MSL)1 is localized to the inner mitochondrial membrane of Arabidopsis thaliana, where it is required for normal mitochondrial responses to oxidative stress. Like Escherichia coli MscS, MSL1 has a pore-lining helix that is kinked. However, in MSL1 this kink is comprised of two charged pore-lining residues, R326 and D327. Using single-channel patch-clamp electrophysiology in E. coli, we show that altering the size and charge of R326 and D327 leads to dramatic changes in channel kinetics. Modest changes in gating pressure were also observed while no effects on channel rectification or conductance were detected. MSL1 channel variants had differing physiological function in E. coli hypoosmotic shock assays, without clear correlation between function and particular channel characteristics. Taken together, these results demonstrate that altering pore-lining residue charge and size disrupts normal channel state stability and gating transitions, and led us to propose the sweet spot model. In this model, the transition to the closed state is facilitated by attraction between R326 and D327 and repulsion between R326 residues of neighboring monomers. In the open state, expansion of the channel reduces inter-monomeric repulsion, rendering open state stability influenced mainly by attractive forces. This work provides insight into how unique charge-charge interactions can be combined with an otherwise conserved structural feature to help modulate MS channel function

Plant Biomechanics: No Pain, No Gain for Birch Tree Stems

Allometric relationships between organism size and shape are often described in developmental or evolutionary terms. A new study characterizes a collapsing birch tree mutant and provides a genetic entry point into the biomechanical control of tree allometry

A Structure-Function Approach to Understanding the Dual Functions of the Plant Mechanosensitive Ion Channel MSL10

MscS‐Like (MSL) 10 is a member of the MscS superfamily of mechanosensitive ion channels and one of 10 MSL proteins in the model flowering plant Arabidopsis thaliana. Unlike Escherichia coli MscS, MSL10 contains 6 transmembrane helices, and only its C‐terminal TM helix shows homology with EcMscS\u27s pore‐lining domain. MSL10 has been shown to provide a mechanosensitive activity in plant cells and Xenopus oocytes. However, its structural organization and function in plants are just beginning to be elucidated

An Accretion-Jet Model for Black Hole Binaries: Interpreting the Spectral and Timing Features of XTE J1118+480

Multi-wavelength observations of the black hole X-ray binary XTE J1118+480 have offered abundant spectral and timing information about the source, and have thus provided serious challenges to theoretical models. We propose a coupled accretion-jet model to interpret the observations. We model the accretion flow as an outer standard thin accretion disk truncated at a transition radius by an inner hot accretion flow. The accretion flow accounts for the observed UV and X-ray emission, but it substantially under-predicts the radio and infrared fluxes, even after we allow for nonthermal electrons in the hot flow. We attribute the latter components to a jet. We model the jet emission by means of the internal shock scenario which is widely employed for gamma-ray bursts. In our accretion-jet model of XTE J1118+480, the jet dominates the radio and infrared emission, the thin disk dominates the UV emission, and the hot flow produces most of the X-ray emission. The optical emission has contributions from all three components: jet, thin disk, and hot flow. The model qualitatively accounts for timing features, such as the intriguing positive and negative time lags between the optical and X-ray emission, and the wavelength-dependent variability amplitude.Comment: 27 pages, 4 figures (one in color); to appear in ApJ in Feb. 200

United in Diversity: Mechanosensitive Ion Channels in Plants

Mechanosensitive (MS) ion channels are a common mechanism for perceiving and responding to mechanical force. This class of mechanoreceptors is capable of transducing membrane tension directly into ion flux. In plant systems, MSion channels have been proposed to play a wide array of roles, from the perception of touch and gravity to the osmotic homeostasis of intracellular organelles. Three families of plant MS ion channels have been identified: the MscS-like (MSL), Mid1-complementing activity (MCA), and two-pore potassium (TPK) families. Channels from these families vary widely in structure and function, localize to multiple cellular compartments, and conduct chloride, calcium, and/or potassium ions. However, they are still likely to represent only a fraction of the MS ion channel diversity in plant systems

PIRATE: A Remotely-Operable Telescope Facility for Research and Education

We introduce PIRATE, a new remotely-operable telescope facility for use in research and education, constructed from 'off-the-shelf' hardware, operated by The Open University. We focus on the PIRATE Mark 1 operational phase where PIRATE was equipped with a widely- used 0.35m Schmidt-Cassegrain system (now replaced with a 0.425m corrected Dall Kirkham astrograph). Situated at the Observatori Astronomic de Mallorca, PIRATE is currently used to follow up potential transiting extrasolar planet candidates produced by the SuperWASP North experiment, as well as to hunt for novae in M31 and other nearby galaxies. It is operated by a mixture of commercially available software and proprietary software developed at the Open University. We discuss problems associated with performing precision time series photometry when using a German Equatorial Mount, investigating the overall performance of such 'off-the-shelf' solutions in both research and teaching applications. We conclude that PIRATE is a cost-effective research facility, and also provides exciting prospects for undergraduate astronomy. PIRATE has broken new ground in offering practical astronomy education to distance-learning students in their own homes.Comment: Accepted for publication in PASP. 14 pages, 11 figure