Hubungan Kepemimpinan Kepala Ruangan Menurut Persepsi Perawat Terhadap Motivasi Kerja Perawat Pelaksana Di Ruang Instalasi Rawat Inap F Blu Rsup Prof. Dr. R.d. Kandou Manado

: Leadership is the ability to provide a constructive influence others to do the business of the cooperative achieve the planned objectives. Motivation to work an employee is usually indicated by a continuous activity, and goal oriented. The purpose of this study is on the analysis of the relationship to the head of the room under the leadership of the nurse\u27s perception of the motivation of nurses in the inpatient department Prof.Dr.R.D. Kandou F BLU Manado. Analytic survey research design using a cross-sectional approach. Popolasi that all nurses in the inpatient space F BLU Prof. Dr. R.D. Kandou Manado. Total sampling using sampling. Data processed through univariate and bivariate analysis using Chi square with Fisher\u27s exact test alternatives. Results obtained by analysis of the probability (p) = 0.003 <α (0.05), which means that Ho is rejected. Conclusion, an association under the leadership of head room nurse perceptions of the work motivation of nurses in the inpatient department Prof.Dr.RDKandou F BLU Manado. Suggestions, for a head irina F would increase the motivation to work more room nurses, and for nurses would be to maintain and further enhance the motivation to work better

Correlations between Right Eye Conjunctival Gene Transcript Levels Reveal Linked Cytokine Responses and the Relationship to Control of Pathogen Load

<div><p>Abundance of <i>IFN-γ, IL-10,</i> and <i>IDO</i> relative to <i>HPRT</i> expression after log₁₀ transformation. Correlation coefficients were calculated by Spearman's rank correlation and trend lines fitted by locally weighted polynomial regression.</p> <p>(A) Strong positive correlation between the conjunctival expression of <i>IDO</i> and <i>IFN-γ</i> in individuals with infection (<i>n =</i> 69) or without infection (<i>n =</i> 238).</p> <p>(B) Strong positive correlation between the expression of <i>IFN-γ</i> and counter inflammatory <i>IL-10</i> in infected (<i>n =</i> 69) and uninfected individuals (<i>n =</i> 238).</p> <p>(C) Relative abundance of <i>IDO</i> and the load of pathogen (chlamydial 16S rRNA) in the conjunctiva. A weak positive correlation was found between pathogen load and <i>IDO</i> expression in clinically normal individuals (<i>n =</i> 42) (mean pathogen loads are lower in this group; <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030266#pmed-0030266-t002" target="_blank">Table 2</a>). In contrast, the relationship between <i>IDO</i> expression and pathogen load is weakly negative or absent in individuals with co-incident clinical signs of trachoma (<i>n</i> = 29) (pathogen loads are highest in this group; <a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030266#pmed-0030266-t002" target="_blank">Table 2</a>).</p> <p>CT, <i>C. trachomatis.</i></p></div

Age Modulation on Right Eye Conjunctival mRNA Expression: Analysis of Covariance

<p>Individuals were categorised using a combination of the presence of clinical signs of active trachoma (TF/TI) and the detection of chlamydial 16S rRNA. Regression models were fitted for the expression of each human mRNA assayed and age (years) within each category. The regression lines for individuals in each category are shown for each of the mRNAs, <i>IDO</i> (top left), <i>IFN-γ</i> (bottom left), <i>IL-10</i> (top right), and <i>FOXP3</i> (bottom right). Green line: clinically normal, uninfected individuals; blue line: clinically normal, infected individuals; red line: clinically diseased, infected individuals; and orange line: clinically diseased, uninfected individuals.</p

Conjunctival Cytokine mRNA Expression in the Right Conjunctivae of Study Participants

<p>RNA was extracted from conjunctival swabs and reverse transcribed, and specific cytokine transcripts were amplified by PCR. Cytokine mRNA expression was normalized against the housekeeping gene <i>HPRT</i>. The response of each individual is shown with the median value (red bar) and the 25th and 75th percentiles (blue bars) for each group. Data were tested for significance by ANOVA and adjusted for age. Individuals are categorised using a combination of the presence of clinical signs of active trachoma (TF/TI) and the detection of chlamydial 16S rRNA. <i>IFN-γ</i> (A) and <i>IDO</i> (B) mRNAs were increased in infected individuals, and they were further increased in those in whom clinical signs coincided with infection compared to clinically normal, uninfected individuals. <i>IL-10</i> mRNA (C) was increased only in those who had clinical infections, while <i>FOXP3</i> mRNA (D) was increased in both clinically infected and clinically diseased, uninfected individuals.</p

TGF-β signaling pathway.

<p>Genes highlighted in yellow are predicted targets of miR differentially regulated in N v TSI (35 miR) (FC>3 p<0.05). Genes highlighted in green are not predicted targets.</p

Venn diagram of differentially expressed miR.

<p>Venn diagram showing the number of differentially expressed (FC>3, p<0.05) miR that are unique or that overlap between the different clinical phenotypes. Selected miR of interest are shown with arrows illustrating whether they are up- or down-regulated in the indicated comparison group. An upward facing arrow indicates up-regulation and a downward facing arrow indicates down-regulation. * miR-23b is up-regulated in N v TS but down-regulated in the TS v TSI comparison group.</p

Results of qPCR differential expression analysis.

*<p>Unadjusted p-values are presented. With no inflation of p-values the chance of finding one or more significant differences in 21 tests = 65.9%. Bonferroni's adjustment indicates critical p-value = 0.002 and assuming outcomes are moderately correlated (r = 0.5) then a critical p-value = 0.01 would be required.</p

Relative abundance of miR in the conjunctiva.

<p>Abundance of all miR tested expressed relative to miR-1274B. Abundance was calculated from cycle threshold values irrespective of sample phenotype.</p

qPCR sample demographic summary including FPC grading scores (0–3) for each phenotypic group.

<p>Footnote: Age ranges between phenotypic groups were not significantly different (Wilcoxon test p>0.05). One individual in the TS group also had a follicular grade (F) of 1, and three individuals in TSI had F3.</p

Sample demographic details before and after quality control exclusion for full array analysis.

<p>FPC grading scores (0–3) are shown for each phenotypic group.</p><p>Footnote: Age ranges between phenotypic groups are not significantly different within and between A and B genecard groups (Wilcoxon test p>0.05). Fewer B genecards were passed filtering as these cards were designed to cover less abundant miR.</p