Apoptosis and Cancer: Mutations Relationship within Caspase 2 Genes in Turk Male Patients

Introduction: Prostate cancer is the most frequently diagnosed male cancer in developed countries. Prostate cancer is the most common cancer diagnosed among men in Turkey, accounting for 42.5% of all cancer cases in men. It ranks second after lung cancer as the underlying cause of cancer death in Turk men. If cancer is a disease where too little apoptosis occurs there are other diseases where too much apoptosis is thought to be part of the problem. Caspases and apoptotic process very important role in modern cancer treatment. Material & Methods: For this reason, we study and analyzed 2 exons of the human caspase 2 gene, using a strategy combining RT-PCR amplification and HRM, followed in the second study, considered to investigate the associations between prostate cancer and caspase 2 gene expression and nucleotide sequence analysis in 12 individulas with prostate cancer and healthy persons. Result: As a result of working, variant-1 and variant-2 of caspase-2 gene mutation was identified in the tissues of the prostate using HRM method. For variant-1, 4 number one mutation was detected in the samples, while for variant-2, 2 numbered examples of mutation has been identified. Conclusion: According to the statistical evaluation in our study, there was significant difference in CSP2 mutations. At the same time, it has been evaluated with one-to-one correspondence tests whether presence of CSP2 mutation has relationship with the development of any clinical symptoms while diagnostic criteria

An EPR Study On Cytosine Irradiated

WOS: 000293594100004In this study, paramagnetic centers over the cytosine were formed by photolysis then these centers were investigated using EPR method. EPR signals were not recorded from non-irradiated the cytosine, but irradiated polycrystalline exhibited complex EPR spectra. For obtaining of cytosine polycrystalline, novel crystallization method was performed on powder cytosine. Effective crystallization conditions were achieved by adjustment of the concentration of the metal ions, chemical solutions, NaCl, KCl, glacial asetic acid, nitric oxide, percloric acid, glutamic acid, and pH of buffer. Cytosine (C4H5N2O) polycrystalline obtained were irradiated with Co-60 - rays at room temperature for 24 and 72 h. At the sample irradiated for 72 h, the paramagnetic centers were determined between 120 and 450 K by X-band EPR spectrometer. The spectra were found to be dependent slightly on temperature. Two cation radicals were determined in the structure and these were called Radicals I and II. The g and hyperfine constants were found to be a(H2a) = 61 G, a(N2) = 9.39 G, a(N1) = 7.15 G, and g(1) = 2.0026 for the Radical I; a(H3) = 10.57 G, a(H1) = 3 G, a(N3) = 6.72 G, a(N1) = 5.36 G for, and g(2) = 2.0034 the Radical II. Copyright (C) 2010 John Wiley & Sons, Ltd.Scientific Research Projects Coordination Center of Selcuk University, Konya/TurkeyThis study was partially supported by the Scientific Research Projects Coordination Center of Selcuk University, Konya/Turkey