Impact of diabetes mellitus on clinical presentation and treatment response of smear positive pulmonary tuberculosis patients

Background: Diabetes mellitus (DM) is a known risk factor for tuberculosis (TB). The aim of this study was to evaluate the effect of DM on clinical presentation and treatment response of sputum positive pulmonary tuberculosis patients.Methods: The present retrospective study was carried out in department of Pulmonary Medicine, Sree Gokulam Medical College and Research Foundation, Trivandrum, between January 2014 to December 2016.Results: Out of 205 subjects enrolled for this study, 73 were diagnosed with DM and 132 patients had no history of DM and were having HbA1c less than 6.5%. Mean HbA1c in DM group at presentation was 10.5 and in patients without DM group was 5.6. Low BMI was more in DM patients. Clinical characteristics at presentation were similar in both the groups. Patients with DM presented after long duration of symptoms (>8 weeks duration) and also with more atypical symptoms. On radiological examination, lower zone involvement and cavity lesions were more in patients with DM which was statistically significant. Clinical picture at the end of 6 months showed improvement in both the groups but radiological improvement was more in patients without DM compared to the other group.Conclusions: Uncontrolled DM is associated with high prevalence of Tuberculosis. Atypical symptoms and atypical radiological findings are more common in DM patients which lead to delayed presentation. Strategies are needed to ensure that optimal care is provided to patients with both diseases.

New distributional records of deep-sea sharks from Indian waters

This paper reports the first documented record of three deepwater sharks from Indian waters i.e., Hexanchus griseus (Hexanchidae), Deania profundorum (Centrophoridae), pygmy false catshark (undescribed) (Pseudotriakidae) and presents a taxonomic account of smooth lanternshark, Etmopterus pusillus (Etmopteridae) and leafscale gulper shark, Centrophorus squamosus (Centrophoridae), caught by hooks & line units operated in the Arabian Sea, west coast of India and landed at Cochin Fisheries Harbour (Kerala), southwest coast of India

Account on the deepsea shrimp Aristaeopsis edwardsiana (Johnson, 1867) from the Indian EEZ

Aristaeopsis edwardsiana (Johnson,1867) is a deepsea shrimp of the continental slope that has not yet been reported in the targeted deepsea crustacean fishery along the Indian coast. An exploratory survey on-board FORV Sagar Sampada in the Arabian sea at a depth of 950 m off Trivandrum (lat. 8 0 28’ N and long. 76 014’ E) yielded a catch of A. edwardsiana at a high catch per unit effort (CPUE) of 14 kg h-1. The biological aspects of this less known deepsea shrimp species such as length frequency distribution, morphometric relations, sex ratio and the additional sexual dimorphism manifested in the antennal scale of males are reported

Shrimps of the family Pandalidae (Caridea) from Indian waters, with new distributional record of Plesionika adensameri (Balss, 1914)

Twenty four species of Pandalid shrimps reported from the Indian waters, of which six genera (Chlorotocella, Chlorotocus, Chlorocurtis, Dorodotes, Heterocarpoides and Stylopandalus) are represented by single species each. The genera, Plesionika and Heterocarpus are represented by eleven and seven species respectively. Plesionika adensameri (Balss, 1914) a deep-sea shrimp hitherto unreported from Indian waters is recorded from west coast of India. Information on some biological aspects of few Pandalid shrimps from Indian waters is also reported in the present paper

Oligonucleotides tethered to a short polyguanylic acid stretch are targeted to macrophages: enhanced antiviral activity of a vesicular stomatitis virus-specific antisense oligonucleotide

The poor membrane permeability of oligonucleotides is one of the major problems of antisense technology. Here we report the construction of designer oligonucleotides for targeted delivery to macrophages. The oligonucleotides tethered to a 10-mer poly(G) sequence at their 3' ends were recognized by scavenger receptors on macrophages and were taken up about 8- to 10-fold as efficiently as those oligonucleotides that either lacked a poly(G) tail or that contained a 10-mer poly(C) tail instead of the poly(G) tail. The enhanced uptake of poly(G) constructs was inhibited in the presence of poly(G) and other known ligands of the scavenger receptor. The bioefficacy of poly(G)-mediated targeting of antisense oligonucleotides (ANS) was demonstrated by using vesicular stomatitis virus (VSV) as a model system. The ability of ANS directed against the translation initiation site of N protein mRNA of VSV to inhibit virus replication was assessed. The ANS with the 10-mer poly(G) sequences (ANS-G) brought about significant inhibition of VSV replication in J774E cells (a murine monocyte/macrophage cell line) and Chinese hamster ovary (CHO) cell transfectants expressing scavenger receptors. The ANS lacking a 10-mer poly(G) stretch were ineffective. The inhibition of VSV replication due to ANS-G was completely abrogated in the presence of 10-mer poly(G), indicating that the antisense effect of the ANS-G molecule was a consequence of scavenger receptor-mediated enhanced uptake. Importantly, antisense molecules linked exclusively by natural phosphodiester bonds were as bioeffective as those synthesized with a mixed backbone of phosphodiester and phosphorothioate. Taken together, these results suggest that macrophage-directed designer ANS against infective agents may simply be obtained by adding a short stretch of guanylic acid sequence to the desired specific ANS during solid-phase synthesis. This nucleic acid-based strategy, which utilizes homogeneous preparation of ANS, may find applications in directed manipulation of macrophage metabolism for a variety of purposes as well as in therapy of a broad spectrum of macrophage-related disorders amenable to the antisense approach

Reduced graphene oxide-multiwalled carbon nanotubes hybrid film with low Pt loading as counter electrode for improved photovoltaic performance of dye-sensitised solar cells

In this work, the role of reduced graphene oxide (rGO) with hyperbranched surfactant and its hybridisation with multiwalled carbon nanotubes (MWCNTs) and platinum (Pt) nanoparticles (NPs) as counter electrode (CE) were investigated to determine the photovoltaic performance of dye-sensitised solar cells (DSSCs). Sodium 1,4-is(neopentyloxy)-3-(neopentyloxycarbonyl)- 1,4-dioxobutane-2-sulphonate (TC14) surfactant was utilised as dispersing and stabilising agent in electrochemical exfoliation to synthesise graphene oxide (GO) as initial solution for rGO production prior to its further hybridisation and fabrication as thin film. A chemical reduction process utilising hydrazine hydrate was conducted to produce rGO due to the low temperature process and water-based GO solution. Subsequently, hybrid solution was prepared by mixing 1 wt% MWCNTs into the produced rGO solution. TC14-rGO and TC14-rGO_MWCNTs hybrid solution were transferred into fluorine-doped tin oxide substrate to fabricate thin film by spraying deposition method. Finally, the CE films were prepared by coating with thin Pt NPs. Photoanode film was prepared by a two-step process: hydrothermal growth method to synthesise titanium dioxide nanowires (TiO2 NWs) and subsequent squeegee method to apply TiO2 NPs. According to solar simulator measurement, the highest energy conversion efficiency (η) was achieved by using CE-based TC14-rGO_MWCNTs/Pt (1.553%), with the highest short current density of 4.424 mA/cm2. The highest η was due to the high conductivity of CE hybrid film and the morphology of fabricated TiO2 NWs/TiO2 NPs. Consequently, the dye adsorption was high, and the photovoltaic performance of DSSCs was increased. This result also showed that rGO and rGO_MWCNTs hybrid can be used as considerable potential candidate materials to replace Pt gradually

Population dynamics of cobia Rachycentron canadum (Linnaeus, 1766) off Cochin coast, south-eastern Arabian Sea

Estimated landings of cobia from the Indian EEZ by the commercial fishing vessels are mostly as bycatch in hooks and line cum gillnet and trawl nets. However, the catches are substantial compared to that reported in several other countries of Asia. The species is also gaining considerable importance as a favoured candidate species for sea farming using cages. Very little is known about the fishery and biological characters such as maturation, fecundity and feeding preferences of cobia in Indian seas. Study indicated a fast growth rate with estimates of L∞ = 184 cm (FL) and K = 2.6 (yr-1) in cobia. The total mortality rate (Z) was estimated as 5.18, natural mortality (M) was 2.01 and fishing mortality (F) was 3.17 with an exploitation rate (E) of 0.61. The length at first capture (Lc50) estimated from the catch curve was 72 cm. Fecundity was found to be high and variable, with mean fecundity estimated as 12,37,545 eggs with a coefficient of variation (CV) of 16.7. As inferred from the oocyte development pattern, spawning activity is brief and fish is classified as synchronous ovulatory type. The growth parameters and condition factor estimated in this study indicate that relatively high growth rates are also possible in wild stocks considering that food is abundant and the environmental temperature is in the optimum range. Feeding preferences indicated balistid fish and shrimps with a wide variety of food items including finfishes, crustaceans and molluscs present

Design and analysis of DLS steel/composite thick-adhernd adhesive joints

The paper describes experimental and numerical techniques to study the structural design and behaviourof thick-adherend DLS joints that are based on steel /steel and steel/composites and epoxy adhesives, withfocus on long overlap joints. A standard fabrication method was followed to produce 60 specimens of various dimensions and materials

Low Genetic Differentiation in the Populations of the Malabar Carp Labeo dussumieri as revealed by Allozymes, Microsatellites and RAPD

The population structure of Labeo dussumieri, an endangered and endemic cyprinid from three riverine locations in the Western Ghats, India was investigated using allozyme, microsatellite and RAPD markers. L. dussumieri samples were obtained from Meenachil, Manimala and Pamba River basins, Kerala. Fourteen (46.7%) out of 30 allozyme loci, seven microsatellite loci and 12 RAPD Operon decamers gave polymorphic pattern. Six allozyme loci (AAT-2*, EST-4*, GLDH*, GPI-2*, G6PDH* and LDH-2*) and three microsatellite loci (LdussG1, MFW19 and Bgon22) exhibited consistent significant deviation from Hardy-Weinberg Equilibrium expectations in different populations after probability level (P<0.05) was adjusted for sequential Bonferroni correction. All the three marker types demonstrated concordant results and various estimates revealed genetic variability within the subpopulations but surprisingly low level (= 0.0034 to 0.0081) of genetic differentiation among L. dussumieri from different river samples. AMOVA analysis also indicated low differentiation among subpopulations. No evidence for a recent genetic bottleneck was observed in L. dussumieri populations based on allozyme and microsatellite data set analysis. Meenachil, Manimala and Pamba Rivers open in to the southern end of Vembanad Lake in Kerala and are connected to each other in the lower reaches through an extensive network of natural canals. Common ancestry in the prehistoric period; and possible mixing of fish populations resulting in high gene flow across the rivers through the lake and interconnecting canals could have been responsible for the lack of significant allelic heterogeneity among the L. dussumieri populations. The stocks from the three rivers do not require different management strategies and for propagation assisted river ranching programme of this species, large effective breeding population can be developed by mixing individuals from three river

Morphometric characteristics of the pelagic stingray Pteroplatytrygon violacea (Bonaparte, 1832) caught off Cochin, southwest coast of India

The morphometric characteristics of the pelagic stingray Pteroplatytrygon violacea (Bonaparte, 1832) are described for the first time from the Indian waters. The specimen was collected at Cochin in August, 2008. The mature male specimen measured 102 cm in total length, 47 cm in disc width, 35 cm in disc length and weighed 2.5 kg. The morphometric characteristics of the specimen were very similar to that described from the North Sea