Antioxidant and toxicity studies of biosynthesized cerium oxide nanoparticles in rats

Funding Information: We would like to thank the Vice Chancellery for Research and Technology, MUMS for financial support (grant no 930954) and facilities.Peer reviewedPublisher PD

Identifying the Research Trends and Subfields of the social manufacturing paradigm

In recent years, studies on the paradigm of social Manufacturing and its applications have been developed as a new production paradigm and have led to the production of diverse and scattered knowledge in this field. Knowing the sub-fields, new topics and the research process of the social production paradigm can be of great help to researchers in this field. The current research has been carried out with the aim of identifying and categorizing research in the field of social Manufacturing, recognizing sub-fields and achieving a coherent view of its research process.This research has investigated the research field of social Manufacturing using bibliometric analysis. The data of this research was collected from 200 articles of the Scopus database and an analysis of the co-occurrence analysis of key words and bibliographic pairs was performed on them, and in this way the sub-fields and the research process of this field were identified.Based on the findings of this study, the research in the field of social Manufacturing has been categorized into 5 clusters and it has also been determined that in recent years, topics such as cloud computing, smart production, blockchain, Internet of Things, social physical cyber systems, innovation systems, society 5.0 and Digital twins have received more attention in research in this field. This research provides a framework of concepts and main topics of interest in the research field of social production, which provides a comprehensive perspective for researchers in this field that can help in choosing their research path

The assessment of lentiviral vectors application for gene transformation in human dermal fibroblasts (HDFs)

چکیده: زمینه و هدف: سلول های بنیادی پرتوان القایی Induced pluripotent stem cells= iPSc))، سلول های اولیه و تمایز نیافته ای هستند که قادر به ایجاد تقریباً هر نوع سلولی در بدن می باشند. هدف از پژوهش حاضر تولید و استفاده مؤثر از وکتور لنتی ویروسی TetO-FUW-OSKM جهت انتقال ژن ها به سلول های فیبروبلاست انسانی ((HDFs= Human fibroblast cells و در نهایت ارزیابی عملکرد این وکتور بود. روش بررسی: در این مطالعه تجربی پس از جداسازی و کشت سلول های HDF، وکتور لنتی ویروسی TetO-FUW-OSKM (به عنوان پلاسمید انتقال دهنده) حاوی ژن های برنامه ریزی مجدد همراه با پلاسمیدهای PsPAX2 و PMDG2 (به عنوان پلاسمیدهای کمکی لازم برای بسته بندی ویروس) به لاین سلولی HEK-293T جهت تولید ویروس ها ترانسفکت شدند. محیط رویی سلول های HEK-293T حاوی ویروس های تولید شده پس از 48 و 72 ساعت برداشت شد و این ویروس ها جهت برنامه ریزی مجدد سلول های HDF به این سلول ها ترانس داکت گردیدند. یافته ها: نتایج این مطالعه نشان دهنده تولید موفقیت آمیز وکتور لنتی ویروسی TetO-FUW-OSKM، کارایی مؤثر روش انتقال ژن با استفاده از وکتورهای لنتی ویروسی و بیان مناسب فاکتورهای رونویسی در سلول های HDF پس از ترانس داکشن بود. نتیجه گیری: با توجه به این یافته ها می توان از وکتورهای لنتی ویروسی جهت انتقال ژن و برنامه ریزی مجدد سلول های بالغ از قبیل HDF در مطالعات بعدی و تولید سلول های iPS استفاده کرد

Comparison between the cultures of human induced pluripotent stem cells (hiPSCs) on feeder- and serum-free system (Matrigel matrix), MEF and HDF feeder cell lines

Human induced pluripotent stem cells (hiPSCs) are a type of pluripotent stem cells artificially derived from an adult somatic cell (typically human fibroblast) by forced expression of specific genes. In recent years, different feeders like inactivated mouse embryonic fibroblasts (MEFs), human dermal fibroblasts (HDFs), and feeder free system have commonly been used for supporting the culture of stem cells in undifferentiated state. In the present work, the culture of hiPSCs and their characterizations on BD Matrigel (feeder- and serum-free system), MEF and HDF feeders using cell culture methods and molecular techniques were evaluated and compared. The isolated HDFs from foreskin samples were reprogrammed to hiPSCs using gene delivery system. Then, the pluripotency ability of hiPSCs cultured on each layer was determined by teratoma formation and immunohistochemical staining. After EBs generation the expression level of three germ layers genes were evaluated by Q-real-time PCR. Also, the cytogenetic stability of hiPSCs cultured on each condition was analyzed by karyotyping and comet assay. Then, the presence of pluripotency antigens were confirmed by Immunocytochemistry (ICC) test and alkaline phosphatase staining. This study were showed culturing of hiPSCs on BD Matrigel, MEF and HDF feeders had normal morphology and could maintain in undifferentiated state for prolonged expansion. The hiPSCs cultured in each system had normal karyotype without any chromosomal abnormalities and the DNA lesions were not observed by comet assay. Moreover, upregulation in three germ layers genes in cultured hiPSCs on each layer (same to ESCs) compare to normal HDFs were observed (p<0.05). The findings of the present work were showed in stem cells culturing especially hiPSCs both MEF and HDF feeders as well as feeder free system like Matrigel are proper despite benefits and disadvantages. Although, MEFs is suitable for supporting of stem cell culturing but it can animal pathogens transferring and inducing immune response. Furthermore, HDFs have homologous source with hiPSCs and can be used as feeder instead of MEF but in therapeutic approaches the cells contamination is a problem. So, this study were suggested feeder free culturing of hiPSCs on Matrigel in supplemented media (without using MEF conditioned medium) resolves these problems and could prepare easy applications of hiPSCs in therapeutic approaches of regenerative medicine such as stem-cell therapy and somatic cell nuclear in further researches

Resveratrol-Mediated Gold-Nanoceria Synthesis as Green Nanomedicine for Phytotherapy of Hepatocellular Carcinoma

In the present study, resveratrol was used to prepare complexes of cerium and nanoceria, also coated with gold (CeO2@Au core-shells) to improve the surface interactions in physiological conditions.The CeO2@Au core-shells were characterized using powder X-ray diffraction (PXRD), Fourier transforms infrared spectroscopy (FTIR), transmission electron microscope (TEM) analysis, dynamic light scattering (DLS) and ζ potential.The experiment was led to the successful synthesis of nanosized CeO2@Au core-shells, although agglomeration of particles caused the distribution of the larger particles. The TEM analysis demonstrated the particles sizes ranged from 20 nm to 170 nm. Moreover, the PXRD analysis showed that both nanoceria and gold with the same crystal systems and space groups. To investigate the anticancer activity of the CeO2@Au core-shells, the cytotoxicity of the nanoparticles was investigated against liver cancerous cell lines (HepG2).The results indicated biosynthesized NCs have significant cellular toxicity properties against HepG2 and could be utilized in hepatocarcinoma therapy. Furthe

Non-communicable diseases in the southwest of Iran: profile and baseline data from the Shahrekord PERSIAN Cohort Study

Background Critical inter-provincial differences within Iran in the pattern of non-communicable diseases (NCDs) and difficulties inherent to identifying prevention methods to reduce mortality from NCDs have challenged the implementation of the provincial health system plan. The Shahrekord Cohort Study (SCS) was designed to address these gaps in Chaharmahal and Bakhtiari, a province of high altitude in the southwest of Iran, characterized by its large Bakhtiari population, along with Fars and Turk ethnicity groups. Methods This ongoing cohort, a prospective, large-scale longitudinal study, includes a unique, rich biobank and was conducted for the first time in Chaharmahal and Bakhtiari Province in Iran. SCS is a part of the PERSIAN (Prospective Epidemiological Research Studies in IrAN) cohort. The study began in 2015, recruited 10075 participants (52.8% female, 47.2% male) from both urban (n=7034) and rural (n=3041) areas, and participants will be annually followed up for at least 15 years. A cross-sectional analysis was conducted using baseline data from the SCS, using descriptive statistics and logistic regression. Data analysis was performed using Stata software. Results The prevalence of NCDs was 9.8% for type 2 diabetes, 17.1% for hypertension, 11.6% for thyroid disease, 0.2% for multiple sclerosis and 5.7, 0.9 and 1.3% for ischemic heart disease, stroke and myocardial infarction, respectively. The prevalence of multimorbidity (>= 2 NCDs) was higher in women (39.1%) than men (24.9%). The means (standard deviations) of age, BMI, systolic blood pressure and fasting blood glucose were 49.5 (9) years, 27.6 (4.6) kg/m(2), 115.4 (17.3) mmHg and 96.7 (27.3) mg/dL, respectively. Logistic regression models showed that older age, female gender, living in an urban area, non-native ethnicity, high wealth index, unemployment, obesity, low physical activity, hypertriglyceridemia, high fasting blood sugar, alkaline urine pH and high systolic and diastolic blood pressure were associated with increased prevalence of NCDs. Conclusions The SCS provides a platform for epidemiological studies that will be useful to better control NCDs in the southwest of Iran and to foster research collaboration. The SCS will be an essential resource for identifying NCD risk factors in this region and designing relevant public health interventions

Association of Angiotensin-Converting Enzyme Inhibitor Gen PolyMorphism with Electrocardiography and Echocardiography Findings in Hemodialysis Patients

Background: Gene polymorphism of angiotensin-converting enzyme (ACE) may be associated with adverse prognosis and increased cardiovascular complications in hemodialysis patients. Objectives: This study aimed to compare the frequency of ACE gene polymorphism in both hemodialysis patients and normal individuals considering echocardiographic findings. Methods: This cross-sectional study included 110 hemodialysis patients (case) and 113 healthy subjects (control). Gene polymorphism of ACE was evaluated in both groups. ECG and echocardiography tests were performed for all patients. Correlations between gene polymorphisms and other variables were analyzed in this study. Polymerase chain reaction (PCR) was used to identify the short deletion allele (D with 190bp), large insertion allele (I with 490bp), and ID genotype which has both alleles. Results: Case and control groups included 46 and 54 female and 64 and 59 male patients, respectively. There were no significant differences between the prevalence of DD, II, and DI alleles of the ACE gene with DI as the most common allele in both groups. No significant differences were found between systolic and diastolic blood pressure and heart rate in DD, DI, and II alleles of the case group. Echocardiographic findings of the patients showed no significant differences between DD, DI, and II genotypes of the case group and intraventricular septal end-diastole (IVSd), MVE vel, MVA vel, MVE/A ratio, MV DT, and MV Dec slope. The mean +/- SD left ventricular end-diastolic diameter (LVEDD) in II, ID, and DD patients were 4.3 +/- 0.72, 4.52 +/- 0.66, and 4.89 +/- 0.93 respectively (P=0.046). Conclusion: The findings of the present study showed that there were no differences in the prevalence of alleles of an ACE gene in hemodialysis patients and control groups. Moreover, no significant associations were observed between alleles of an ACE gene in the patients' group and echocardiographic findings except in left ventricular end-diastolic diameter

Transtheoretical model of health behavioral change: A systematic review

Background: Transtheoretical model (TTM) is one of the most commonly used methods in behavioral change modeling. The aim of this study was to conduct a systematic review (SR) to determine research gaps with regard to this template with an emphasis on intervention for patients with chronic diseases (CDs). Materials and Methods: ISI-WOS, Scopus, PubMed, SID, and Magiran databases were examined systematically and on the basis of defined criteria. Titles, abstracts, and full texts of articles retrieved were examined for the presence of defined criteria. Then finalized articles were analyzed in consensus meetings. After that, references of selected articles and full text of those meeting the criteria were also analyzed Results: We screened 103 articles, excluded 27 in abstract review and 34 in full-text review, leaving 42 articles for critical appraisal. Then the references of these 42 articles were also screened. Fifty articles were excluded on abstract review and 5 on full-text review, leaving 15 articles. The result of the analysis of 57 final articles of this SR determined that 28 articles were about aspects of TTM and 5 stages of change were the most commonly used aspect. Eight articles used TTM in intervention about CDs. A total of 21 articles examined TTM's pros and cons, most of which were about TTM's pros. Conclusions: The majority of studies focused on the effectiveness of TTM on the behavioral change management. This finding supported the hypothesis that TTM can be applied in the prevention of CDs

The effect of nutrition education course on awareness of obese and overweight female 1st-year High School students of Isfahan based on transtheoretical model of behavioral change

Background/Aim: Today, the problem of obesity is a chronic condition that affects all age groups in developed and developing countries. Since adolescence is a transitional period and behavioral patterns formed in this period affect people in their adult years, attention to obesity and extra weight in this age group is more important than other groups. Therefore, this study aims to investigate the effects of nutrition education course on awareness of female 1st-year high school students of Isfahan based on transtheoretical model ( TTM) of behavioral change. Materials and Methods: In this semi-empirical study, 64 female 1st-year high school students with weight problem were selected using nonpercent stratified sampling and divided into two control and test groups. Data gathering was carried out using researcher-made nutrition awareness and stages of change questionnaire whose validity and reliability was confirmed. Participants in the test group participated in a 2-month education with sessions every 2 weeks and received one brochure and 3 educational massages each week. Data were analyzed using independent t-test and Mann–Whitney test. Results: The results showed that the average nutrition awareness score of students in test group was significantly higher than control group after intervention. Students in test group were also in higher stages of change compared to control group after intervention. Conclusion: The results show that TTM was effective in changing nutritional behavior in students