INVESTIGATION THE ROLE OF MEPXH1 (HIS139ARG) POLYMORPHISM ON NUMBER OF EXACERBATIONS AND DISEASE SEVERITY IN CHRONIC OBSTRUCTIVE PULMONARY DISEASE IN A SMOKER POPULATION

The purpose of this study was to examine investigation the role of mEPXH1 (His139Arg) polymorphisms on number of exacerbations and disease severity in chronic obstructive pulmonary disease in a smoker population. Chronic obstructive pulmonary disease (COPD) has become the fourth most common single cause of morbidity, and its prevalence is increasing worldwide. It is a syndrome composed of chronic bronchitis, small airways disease (bronchiolitis), and emphysema, in varying proportions between affected individuals. The study was performed cohort and prospectively. The population consist of 213 patients with COPD disease. Genotyping of mEPXH1 was performed using multiplex PCR. Data analysis included, Pearson’s r correlations, regression analysis, ANOVA analyses, Tukey, test for comparison and SPSS software (package of Spss / pc + + ver18). The results showed that there is not relationship between polymorphisms of mEPXH1 and number of exacerbations. According the results, there is not significant relationship between polymorphisms of mEPXH1 and disease severity. Also there is not significant relationship between mEPXH1 and disease in COPD patients on basis parameters of spirometery and oxidative stress in COPD patients

Prevalence of micro-aspiration of bile acids in patients with primary lung cancer: A cross-sectional study

BACKGROUND: Lung cancer remains a serious public healthproblem and is the first cause of cancer-related death worldwide. There is some evidence suggests that bile acid micro-aspiration may contribute to the development of lung diseases. This study aimed to assess the prevalence of microaspiration of bile acids in patients with primary lung cancer.METHODS: In a cross-sectional study, 52 patients with primary lung cancer referred to a teaching hospital affiliated with Kerman University of Medical Sciences, Kerman, Iran were enrolled. Patients with pathology-confirmed lung cancer who did not receive specific treatment were included in the present study. All patients underwent bronchoscopy and the levels of bile acid was assessed in their Broncho-Alveolar Lavage (BAL) samples.RESULTS: According to the results, 53.85% of patients were in the age group of 40 to 59 years. Of the participants, 88.46% were male, 82.69% were smokers, and 69.23% were opium addicted. The most common presenting clinical symptoms of patients were heartburn (61.55%), hoarseness (17.31%), and epigastric pain (9.61%), respectively. Ninety-two point thirty two percent of patients had endobronchial lesions in bronchoscopy. Squamous cell carcinoma, small-cell lung carcinoma and adenocarcinoma accounts for 48.08%, 34.61% and 17.31% of all cases of lung cancer, respectively. Bile acids were found in the BAL sample of all patients with primary lung cancer. The mean Bile acids levels in patients were 63.42 (SD=7.03) μmol/Lit.CONCLUSION: According to the results of present study, there was a micro-aspiration of bile acids in all patients with primary lung cancer that may participate in shaping early events in the etiology of primary lung cancer. It seems that developing clinical strategies preventing the micro-aspiration of bile acids into the lungs could remove a key potential trigger in this process

Detection of Helicobacter Pylori in Bronchoalveolar Lavage of Patients With Chronic Obstructive Pulmonary Disease by Real Time Polymerase Chain Reaction

Background: Chronic obstructive pulmonary disease (COPD) is one of the most important causes of disability and mortality in the world. Although cigarette smoking and environmental pollutants have been recognized as the major causes of COPD, the role of infection in the pathogenesis and progression of COPD has also been reported. Objectives: The aim of the present study was to find the relationship between Helicobacter Pylori infection and COPD through anti H. pylori IgG serology, real time PCR of bronchoalveolar lavage and trans bronchial biopsy urease tests. Patients and Methods: This descriptive cross-sectional study was carried out on 60 adults with COPD. After obtaining the patient’s history, physical examination, spirometry and confirmation of COPD diagnosis by pulmonologist, subjects were selected through convenience sampling. In order to determine the severity and prognosis of disease, the global initiative for chronic obstructive lung disease (GOLD) criteria and BODE index were used. Subjects underwent bronchoscopy for obtaining bronchoalveolar lavage (BAL) samples and biopsy was performed. Biopsy and BAL samples were investigated respectively by urease test and real time PCR. Moreover, patients’ serum samples were serologically studied for detection of anti H. pylori IgG. Results: Mean age of the participants was 60.65 ± 9.15 years, and 25% were female and 75% were male. The prevalence rate of H. pylori in COPD patients was 10% according to real time PCR, 88.3% according to the serology test and 0% based on the urease test. According to the results of PCR and considering the severity of disease based on the GOLD criteria, from those with a positive PCR, one patient (16.6%) had very severe obstruction, three (50%) had severe obstruction and two patients (33.3%) had moderate obstruction. The relationship between H. pylori presence (based on PCR) and disease severity and prognosis was not statistically significant. Conclusions: These findings can justify the hypothesis of direct injury and chronic inflammation via inhalation and aspiration resulting in H. pylori colonization. In fact, it is thought that H. Pylori infection, beside the host genetic vulnerability and other environmental risk factors might make the patient susceptible to COPD or lead to COPD worsening. Although we found H. pylori infection in some patients with COPD, the results of this study, could not explain the pathogenic mechanisms of COPD