A Concise Review of Chromatographic Methods for the Analysis of Benzene and its Metabolites

Benzene is an important pollutant with carcinogenic potential. The main sources of benzene exposure include its unabated industrial use, gasoline vapors, emissions from engines and tobacco smoke. Environmental and occupational exposure of benzene has been associated with serious health hazards. Analysis of benzene is therefore important for the monitoring of environmental quality. The metabolites of benzene, especially S-phenyl mercapturic acid (S-PMA) and trans, trans-muconic acid (t,t-MA) have been used as sensitive biomarkers of benzene exposure. Estimation of un-metabolized benzene in urine has also been suggested as a more reliable indicator of its exposure. This review describes various chromatographic methods for the estimation of benzene and its metabolites in different environmental and biological samples

ArraySolver: An Algorithm for Colour-Coded Graphical Display and Wilcoxon Signed-Rank Statistics for Comparing Microarray Gene Expression Data

The massive surge in the production of microarray data poses a great challenge for proper analysis and interpretation. In recent years numerous computational tools have been developed to extract meaningful interpretation of microarray gene expression data. However, a convenient tool for two-groups comparison of microarray data is still lacking and users have to rely on commercial statistical packages that might be costly and require special skills, in addition to extra time and effort for transferring data from one platform to other. Various statistical methods, including the t-test, analysis of variance, Pearson test and Mann–Whitney U test, have been reported for comparing microarray data, whereas the utilization of the Wilcoxon signed-rank test, which is an appropriate test for two-groups comparison of gene expression data, has largely been neglected in microarray studies. The aim of this investigation was to build an integrated tool, ArraySolver, for colour-coded graphical display and comparison of gene expression data using the Wilcoxon signed-rank test. The results of software validation showed similar outputs with ArraySolver and SPSS for large datasets. Whereas the former program appeared to be more accurate for 25 or fewer pairs (n ≤ 25), suggesting its potential application in analysing molecular signatures that usually contain small numbers of genes. The main advantages of ArraySolver are easy data selection, convenient report format, accurate statistics and the familiar Excel platform

Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis.

This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 microl of reaction mixture (4 x 10(-4) M luminol+50 microg opsonized zymosan+0.1% gelatin in Hank's balanced salt solution) and the chemiluminescence signal was measured in a luminometer at 37 degrees C. Fecal peritonitis caused a significant leukocytopenia (3540+/-297 mm(-3) versus control value of 7525+/-711 mm(-3), p < 0.001) accompanied by massive infiltration of PMNs in the peritoneal cavity (34700+/-4006 versus 7325+/-425 mm(-3), p < 0.001). The phagocytic activity of circulating blood PMNs was down-regulated whereas a significant up-regulation was observed in the activity of PMNs from peritoneal fluid. In conclusion, this study clearly demonstrates sepsis-induced alterations in both blood and peritoneal fluid PMNs and their quantitative assessment may be helpful in disease evaluation and designing effective therapies

Effect of Fluconazole on Phagocytic Response of Polymorphonuclear Leukocytes in a Rat Model of Acute Sepsis

Recently, fluconazole (FLZ) has been shown to improve survival and reduce multiorgan failure in experimental and clinical septic shock. The mechanism by which FLZ affords protection against sepsis remains obscure. This study examines the effect of FLZ on phagocytic activity of polymorphonuclear leukocytes (PMNs) in a rat model of septic shock by inducing fecal peritonitis in male Wistar rats using intraperitoneal instillation (1 mL/kg) of fecal suspension in saline (1:1 w/v). Sham control rats received sterile fecal suspension and vehicle treatment. FLZ was administered in the doses of 0, 3, 10, and 30 mg/kg by gavage 30 minutes before fecal instillation. The samples of peritoneal fluid were collected 8 hours following fecal inoculation for the evaluation of phagocytic response of PMNs using zymosan-induced luminol-dependent chemiluminescence (CL). Fecal peritonitis caused massive infiltration of PMNs in the peritoneal cavity (ANOVA F(4.45) = 6.322, P < .001). Although FLZ reduced the infiltration of PMNs, this effect was neither significant nor dose dependent. The actual CL response was significantly higher in the peritoneal fluid of rats subjected to peritonitis, which was significantly and dose-dependently attenuated by FLZ treatment (ANOVA F(4.45) = 11.048, P < .001). Normalization of CL response for 1000 PMNs revealed that FLZ dose-dependently albeit insignificantly reduced the activity of PMNs. The high dose of FLZ caused 2.29-fold decrement in the area under curve (AUC) pertaining to cumulative CL response. The findings of this study suggest that FLZ protects rats against septic shock by inhibiting PMN-mediated inflammatory cascade without compromising their phagocytic activity

Developing of Institutional Repository in Public Sector University Libraries of Punjab, Pakistan

The target of this analysis to discover the current status of open source institutional repository software’s usage in the public sector university libraries of Punjab, Pakistan. Survey method was used by the researchers and a survey was adopted for information gathering. The target population of the investigation was library professionals of public sector universities in Punjab. Data was gathered from a sample of 135 university librarians using a convenience sampling approach. The analyses explored in which majority of library professionals have incorporated or are currently in the process of incorporating institutional repositories (IR) into their respective libraries which shows that library professionals are well aware about the importance of development/need of IR in this digital age. Further, this study revealed that most of the professionals preferred to use open source software i.e. D-Space for development of IR. Moreover, library professionals are of the view that IR software is a vital tool to organize the digital content/material. Further, IR software provides easy access to the users and enables them to provide efficient and effective services to their users. Furthermore, this study highlights that majority of participants were preferred to use those software which have the facility of customization, E-mail based sharing of books. Library professionals are satisfied with that IR software which is freely available, provide format for conversion of different digital files, and publishing digital contents. The study also highlights barriers which faced by majority of respondents i.e. selection of proper software while developing the Institutional Repository. The respondents also ranked lack of budget is another major barrier that forbade the development of IR followed by the selection of scanner technology, selection of material for digitization, institutional repository knowledge. The investigators will explore additional avenues for additional investigation through the lens of institutional repositories (IR)

DNA Barcodes of Arabian Partridge and Philby’s Rock Partridge: Implications for Phylogeny and Species Identification

Recently, DNA barcoding based on mitochondrial cytochrome c oxidase subunit I (COI) has gained wide attention because of simplicity and robustness of these barcodes for species identification including birds. The current GenBank records show the COI barcodes of only one species, chukar partridge (Alectoris chukar), of the Alectoris genus. In this study, we sequenced the 694 bp segment of COI gene of the two species including, Arabian partridge (Alectoris melanocephala) and Philby’s rock partridge (Alectoris philbyi) of the same genus. We also compared these sequences with earlier published barcodes of chukar partridge. The pair-wise sequence comparison showed a total of 53 variable sites across all the 9 sequences from 3 species. Within-species variable sites were found to be 4 (Alectoris chukar), 0 (Alectoris philbyi) and 3 (Alectoris melanocephala). The genetic distances among the 9 individuals varied from 0.000 to 0.056. Phylogenetic analysis using COI barcodes clearly discriminated the 3 species, while Alectoris chukar was found to be more closely related to Alectoris philbyi. Similar differentiation was also observed using 1155 bp mitochondrial control region (CR) sequences suggesting the efficiency of COI gene for phylogenetic reconstruction and interspecific identification. This is the first study reporting the barcodes of Arabian partridge and Philby’s rock partridge

Enhanced Handover Mechanism in Long Term Evolution (LTE) Networks

Femtocell is a low power base station, wireless access point designed especially for homes and small organizations. It is promising technology for operators to improve their capacity and for users to give indoor coverage. As mobile users are increasing day by day so the legacy system is unable to provide such a high data rates to all these users. In this case femtocells play a key role to offload the data traffic from macro base station. The implementation of femtocell has posed so many challenges like interference, localization, access control and mobility management. The aim of this paper is to present an enhanced algorithm for handover in Hand-In scenario. In already existing algorithms handover is decided on the basis of a single parameter but here we have simulated an algorithm that considers multiple parameters instead of a single parameter for handover. Through this algorithm, the most suitable femtocell will be selected for handover, hence number of handovers will be decreased. Simulation results show that the system performance has been improved.

Frequency of raised red cell distribution width in acute exacerbation of COPD patients

Objective: objective of our study is to investigate and establish the frequency of raised red cell distribution width in acute exacerbation of COPD patients. Study design: Cross sectional study Place and Duration of study: This study was conducted in pulmonology department of Nishtar Hospital Multan from January 2016 to December 2017. Methods: Total 384 patients were selected by non-probability consecutive sampling and approval of study was taken from ethical committee of the institution. Written permission of study was signed by every patient enrolled in study. Numerical variables like age, weight, white blood cells, platelets, MCV, hemoglobin (Hb) and duration of COPD were analyzed statically by taking their mean and standard deviation and t-test was applied to test their significance. Non numerical variables like gender, anemia, thrombocytopenia, income and area of living were statistically analyzed by taking their frequency and chi square test was applied to test their significance. While the outcome variable red cell distribution width was assessed by 3-part hematology chamber. Results: Overall, there were 100% (n=384) patients were included, in this study, both genders. There were 60.7% (n=233) males and 39.3% (n=151) females. 72.1% (n=277) patients belonged to urban areas while 27.9% (n=107) belonged to rural areas. 69% (n=265) had good income and 31% (n=119) had low income. The mean age and BMI of the patients was 65.22±6.45 years and 26.21±2.18 kg/m2 respectively. There were 26% (n=100) patients between 40-60 years while majority of the patients i.e. 74% (n=284) between 61-80 years of age. The distribution of BMI of the patients observed as; 39.3% (n=151) patients between 18-25 kg/m2 and 60.7% (n=233) patients between 26-32 kg/m2. The main outcome variable of this study was red cell distribution width. The Mean±S.D of red cell distribution width was 14.03±1.62%. While, red cell distribution width noted as normal and raised; 54.9% (n=211) and 45.1% (n=173) respectively. Conclusion: Study concluded that there was significant prevalence of raised red cell distribution width (RDW) in acute exacerbation of COPD patients. This easy, inexpensive and quick parameter should be investigated further. So it can be used as prognostic marker and predictor of mortality in acute exacerbation of COPD patients. Key words: Acute exacerbation of COPD (AECOPD), red cell distribution width (RDW), COPD, Inflammation

The Influence of Plastic Mulching Materials and Sowing Dates on the Yield and Yield Components of Pea (Pisum sativum L.)

The use of plastic mulches affects the microclimate in the vicinity of the plant by modifying the radiation on the soil surface and reduce the loss of water. Pea is more nutritious and 2nd food source after cereals at Pakistan, but due to lake of proper management, yield is still not up to mark as compare to developed world. To reduce this problem, a field experiment was conducted to investigate the effect of mulching material and sowing dates on the yield of Pea. The study consisted of three different mulching materials (Polythene Black, Polythene Blue and Polythene Brown) with three different sowing dates (1st, 15th and 30th October) arranged in a Randomized Complete Block Design with three replications. The results showed that different sowing dates and mulching materials had significant effect on the yield parameters of Pea. Regarding sowing dates, maximum days to pod formation (8.08 days), pods plant?1 (21.25), Pod weight (18.84 g) and pod yield (4.39 tons ha?1) were noted in pea sown on October 1st. Whereas, minimum data of days to pod formation (3.08 days), pod plant?1 (15.34), pod weight (13.17 g) and pod yields (2.69tons ha?1) were recorded in pea sown on October 30th. In case of mulching types maximum number of pods plant?1 (19.00), pod length (6.89 cm), seeds pod?1 (7.78), pod weight (17.34 g) and pod yield (3.69 tons ha?1) were observed in pea mulched with Polythene Black, while minimum pods plant-1, pod length, seeds pod-1, pod weight and pod yield were found in control. The study suggested that pea should be sown on October 1st while mulched with Polythene Black to produce maximum yield and production in agro environment of district Peshawar

Smart Relay Selection Scheme Based on Fuzzy Logic with Optimal Power Allocation and Adaptive Data Rate Assignment

In this paper fuzzy logic-based algorithm with improved process of relay selection is presented which not only allocate optimal power for transmission but also help in choosing adaptive data rate. This algorithm utilizes channel gain, cooperative gain and signal to noise ratio with two cases considered in this paper: In case-I nodes do not have their geographical location information while in case-II nodes are having their geographical location information. From Monte Carlo simulations, it can be observed that both cases improve the selection process along with data rate assignment and power allocation, but case-II is the most reliable with almost zero probability of error at the cost of computational complexity which is 10 times more than case-I