12 政治学の対象としての国家－政治権力論序説－ ＜神奈川大学創立三五周年記念論文集＞

第一

〔研究ノート〕慢性腎臓病モデルラットの骨密度維持に対する食餌アルギニンあるいは大豆イソフラボン抽出物投与の有効性評価に関する基礎的研究

The present study was carried out to determine whether dietary isoflavone and arginine could preserve the renal function and prevent renal injury and bone mineral density loss in the chronic kidney disease model rat. We orally administered 360mg/kg BW of adenine suspended in methylcellulose to ten-week-old male Wistar rats for six days to generate a chronic kidney disease(CKD)model. As a control, methylcellulose alone was administered to another group of rats(Intact). The rats received 15g/day of either the control diet(group CA)or the experimental diet supplemented with isoflavone(group IF)or L-Arginine(group Arg)or isoflavone plus L-Arginine simultaneously(group IFA)for 10 weeks. Group CA still showed the symptoms of chronic kidney disease, such as renal swelling, a decreased CCr, elevated BUN and increased urinary NAG activity compared to the Intact rats that had not received adenine treatment. The results were as follows: Compared with group CA, 1)Group IF showed a higher femoral cancellous-BMD, but no improvement in the renal function estimated by the CCr and BUN or the renal injury levels estimated by the urinary protein excretion and urinary NAG activity. 2)Group Arg showed a higher CCr, lower BUN and lower urinary protein excretion; however, the femoral cancellous-BMD did not increase. 3)Group IFA showed a lower urinary protein excretion level and urinary NAG activity and higher femoral cancellous-BMD. These results suggest that isoflavone and arginine are effective dietary agents for suppressing renal failure and maintaining the BMD in a rat model of chronic kidney disease

Altered Trabecular Bone Structure and Delayed Cartilage Degeneration in the Knees of Collagen VI Null Mice

Mutation or loss of collagen VI has been linked to a variety of musculoskeletal abnormalities, particularly muscular dystrophies, tissue ossification and/or fibrosis, and hip osteoarthritis. However, the role of collagen VI in bone and cartilage structure and function in the knee is unknown. In this study, we examined the role of collagen VI in the morphology and physical properties of bone and cartilage in the knee joint of Col6a1−/− mice by micro-computed tomography (microCT), histology, atomic force microscopy (AFM), and scanning microphotolysis (SCAMP). Col6a1−/− mice showed significant differences in trabecular bone structure, with lower bone volume, connectivity density, trabecular number, and trabecular thickness but higher structure model index and trabecular separation compared to Col6a1+/+ mice. Subchondral bone thickness and mineral content increased significantly with age in Col6a1+/+ mice, but not in Col6a1−/− mice. Col6a1−/− mice had lower cartilage degradation scores, but developed early, severe osteophytes compared to Col6a1+/+mice. In both groups, cartilage roughness increased with age, but neither the frictional coefficient nor compressive modulus of the cartilage changed with age or genotype, as measured by AFM. Cartilage diffusivity, measured via SCAMP, varied minimally with age or genotype. The absence of type VI collagen has profound effects on knee joint structure and morphometry, yet minimal influences on the physical properties of the cartilage. Together with previous studies showing accelerated hip osteoarthritis in Col6a1−/− mice, these findings suggest different roles for collagen VI at different sites in the body, consistent with clinical data

Studies on novel Elastin-binding proteins in human and porcine plasmas

博士（農学）東京農工大