Simple t-distribution Based Tests for Meta-Analysis

The variance function of the optimal estimator of the overall mean in a heteroscedastic one-way ANOVA model is dominated by positive semi-definite quadratic functions. This makes it possible to develop closely related tests on the nullity of the overall mean parameter, in one-way fifixed and random effects ANOVA models, which make use of the quantiles of the t-distribution. These tests are founded on the convexity arguments similar to Hartung (1976). Simulation results indicate that the proposed tests attain type I error rates which are far more acceptable than those of the commonly used tests

On the efficient numerical solution of lattice systems with low-order couplings

We apply the Quasi Monte Carlo (QMC) and recursive numerical integration methods to evaluate the Euclidean, discretized time path-integral for the quantum mechanical anharmonic oscillator and a topological quantum mechanical rotor model. For the anharmonic oscillator both methods outperform standard Markov Chain Monte Carlo methods and show a significantly improved error scaling. For the quantum mechanical rotor we could, however, not find a successful way employing QMC. On the other hand, the recursive numerical integration method works extremely well for this model and shows an at least exponentially fast error scaling

Polymorphism of the tumor necrosis factor beta gene in systemic lupus erythematosus

We investigated the Nco I restriction fragment length polymorphism (RFLP) of the tumor necrosis factor beta (TNFB) gene in 173 patients with systemic lupus erythematosus (SLE), 192 unrelated healthy controls, and eleven panel families, all of German origin. The phenotype frequency of the TNFB*I allele was significantly increased in patients compared to controls (63.6% vs 47.1%, RR = 1.96, p <0.002). The results of a two-point haplotype statistical analysis between TNFB and HLA alleles show that there is linkage disequilibrium between TNFB*I and HLA-A1, Cw7, B8, DR3, DQ2, and C4A DE. The frequency of TNFB*I was compared in SLE patients and controls in the presence or absence of each of these alleles. TNFB*I is increased in patients over controls only in the presence of the mentioned alleles. Therefore, the whole haplotypeA1, Cw7, B8, TNFB* I, C4A DE, DR3, DQ2 is increased in patients and it cannot be determined which of the genes carried by this haplotype is responsible for the susceptibility to SLE. In addition, two-locus associations were analyzed in 192 unrelated healthy controls for TNFB and class I alleles typed by serology, and for TNFB and class II alleles typed by polymerase chain reaction/oligonucleotide probes. We found positive linkage disequilibrium between TNFB*I and the following alleles: HLA-A24, HLA-B8, DRBI*0301, DRBI*ll04, DRBI*1302, DQAI*0501, DQBI*0201, DQBI*0604, and DPBI*OIO1. TNFB*2 is associated with HLA-B7, DRBI*1501, and DQB I *0602

Enantioselective Olefin Metathesis with Cyclometalated Ruthenium Complexes

The success of enantioselective olefin metathesis relies on the design of enantioenriched alkylidene complexes capable of transferring stereochemical information from the catalyst structure to the reactants. Cyclometalation of the NHC ligand has proven to be a successful strategy to incorporate stereogenic atoms into the catalyst structure. Enantioenriched complexes incorporating this design element catalyze highly Z- and enantioselective asymmetric ring opening/cross metathesis (AROCM) of norbornenes and cyclobutenes, and the difference in ring strain between these two substrates leads to different propagating species in the catalytic cycle. Asymmetric ring closing metathesis (ARCM) of a challenging class of prochiral trienes has also been achieved. The extent of reversibility and effect of reaction setup was also explored. Finally, promising levels of enantioselectivity in an unprecedented Z-selective asymmetric cross metathesis (ACM) of a prochiral 1,4-diene was demonstrated

VLT Diffraction Limited Imaging and Spectroscopy in the NIR: Weighing the black hole in Centaurus A with NACO

We present high spatial resolution near-infrared spectra and images of the nucleus of Centaurus A (NGC 5128) obtained with NAOS-CONICA at the VLT. The adaptive optics corrected data have a spatial resolution of 0.06" (FWHM) in K- and 0.11" in H-band, four times higher than previous studies. The observed gas motions suggest a kinematically hot disk which is orbiting a central object and is oriented nearly perpendicular to the nuclear jet. We model the central rotation and velocity dispersion curves of the [FeII] gas orbiting in the combined potential of the stellar mass and the (dominant) black hole. Our physically most plausible model, a dynamically hot and geometrically thin gas disk, yields a black hole mass of M_bh = (6.1 +0.6/-0.8) 10^7 M_sun. As the physical state of the gas is not well understood, we also consider two limiting cases: first a cold disk model, which completely neglects the velocity dispersion; it yields an M_bh estimate that is almost two times lower. The other extreme case is to model a spherical gas distribution in hydrostatic equilibrium through Jeans equation. Compared to the hot disk model the best-fit black hole mass increases by a factor of 1.5. This wide mass range spanned by the limiting cases shows how important the gas physics is even for high resolution data. Our overall best-fitting black hole mass is a factor of 2-4 lower than previous measurements. With our revised M_bh estimate, Cen A's offset from the M_bh-sigma relation is significantly reduced; it falls above this relation by a factor of ~2, which is close to the intrinsic scatter of this relation. (Abridged)Comment: 12 pages, 14 figures, including minor changes following the referee report; accepted for publication in The Astrophysical Journa

Production and characterization of monoclonal antibodies to the extracellular domain of PO

Seven monoclonal antibodies were raised against the immunoglobulin-like extracellular domain of PO (POED), the major protein of peripheral nervous system myelin. Mice were immunized with purified recombinant rat PO-ED. After fusion, 7 clones (POI-P07) recognizing either recombinant, rat, mouse, or human PO-ED were selected by ELlS A and were characterized by Western blot, immunohistochemistry, and a competition assay. Antibodies belonged to the IgG or IgM class, and P04-P07, reacted with PO in fresh-frozen and paraffin-embedded sections of human or rat peripheral nerve, but not with myelin proteins of the central nervous system of either species. Epitope specificity of the antibodies was determined by a competition enzyme-linked immunosorbent assay (ELISA) and a direct ELlS A using short synthetic peptides spanning the entire extracellular domain of PO. These assays showed that POl and P02 exhibiting the same reaction pattern in Western blot and immunohistochemistry reacted with different distant epitopes of PO. Furthermore, the monoclonal antibodies P05 and P06 recognized 2 different epitopes in close proximity within the neuritogenic extracellular sequence of PO. This panel of monoclonal antibodies, each binding to a different epitope of the extracellular domain of PO, will be useful for in vitro and in vivo studies designed to explore the role of PO during myelination and in demyelinating diseases of the peripheral nervous system

An EC-Earth coupled atmosphere–ocean single-column model (AOSCM.v1_EC-Earth3) for studying coupled marine and polar processes

Single-column models (SCMs) have been used as tools to help develop numerical weather prediction and global climate models for several decades. SCMs decouple small-scale processes from large-scale forcing, which allows the testing of physical parameterisations in a controlled environment with reduced computational cost. Typically, either the ocean, sea ice or atmosphere is fully modelled and assumptions have to be made regarding the boundary conditions from other subsystems, adding a potential source of error. Here, we present a fully coupled atmosphere–ocean SCM (AOSCM), which is based on the global climate model EC-Earth3. The initial configuration of the AOSCM consists of the Nucleus for European Modelling of the Ocean (NEMO3.6) (ocean), the Louvain-la-Neuve Sea Ice Model (LIM3) (sea ice), the Open Integrated Forecasting System (OpenIFS) cycle 40r1 (atmosphere), and OASIS3-MCT (coupler).Results from the AOSCM are presented at three locations: the tropical Atlantic, the midlatitude Pacific and the Arctic. At all three locations, in situ observations are available for comparison. We find that the coupled AOSCM can capture the observed atmospheric and oceanic evolution based on comparisons with buoy data, soundings and ship-based observations. The model evolution is sensitive to the initial conditions and forcing data imposed on the column. Comparing coupled and uncoupled configurations of the model can help disentangle model feedbacks. We demonstrate that the AOSCM in the current set-up is a valuable tool to advance our understanding in marine and polar boundary layer processes and the interactions between the individual components of the system (atmosphere, sea ice and ocean).</p