983 research outputs found
Regulation of methanol utilisation pathway genes in yeasts
Methylotrophic yeasts such as Candida boidinii, Hansenula polymorpha, Pichia methanolica and Pichia pastoris are an emerging group of eukaryotic hosts for recombinant protein production with an ever increasing number of applications during the last 30 years. Their applications are linked to the use of strong methanol-inducible promoters derived from genes of the methanol utilisation pathway. These promoters are tightly regulated, highly repressed in presence of non-limiting concentrations of glucose in the medium and strongly induced if methanol is used as carbon source. Several factors involved in this tight control and their regulatory effects have been described so far. This review summarises available data about the regulation of promoters from methanol utilisation pathway genes. Furthermore, the role of cis and trans acting factors (e.g. transcription factors, glucose processing enzymes) in the expression of methanol utilisation pathway genes is reviewed both in the context of the native cell environment as well as in heterologous hosts
Simbol-X Hard X-ray Focusing Mirrors: Results Obtained During the Phase A Study
Simbol-X will push grazing incidence imaging up to 80 keV, providing a strong
improvement both in sensitivity and angular resolution compared to all
instruments that have operated so far above 10 keV. The superb hard X-ray
imaging capability will be guaranteed by a mirror module of 100 electroformed
Nickel shells with a multilayer reflecting coating. Here we will describe the
technogical development and solutions adopted for the fabrication of the mirror
module, that must guarantee an Half Energy Width (HEW) better than 20 arcsec
from 0.5 up to 30 keV and a goal of 40 arcsec at 60 keV. During the phase A,
terminated at the end of 2008, we have developed three engineering models with
two, two and three shells, respectively. The most critical aspects in the
development of the Simbol-X mirrors are i) the production of the 100 mandrels
with very good surface quality within the timeline of the mission; ii) the
replication of shells that must be very thin (a factor of 2 thinner than those
of XMM-Newton) and still have very good image quality up to 80 keV; iii) the
development of an integration process that allows us to integrate these very
thin mirrors maintaining their intrinsic good image quality. The Phase A study
has shown that we can fabricate the mandrels with the needed quality and that
we have developed a valid integration process. The shells that we have produced
so far have a quite good image quality, e.g. HEW <~30 arcsec at 30 keV, and
effective area. However, we still need to make some improvements to reach the
requirements. We will briefly present these results and discuss the possible
improvements that we will investigate during phase B.Comment: 6 pages, 3 figures, invited talk at the conference "2nd International
Simbol-X Symposium", Paris, 2-5 december, 200
The imaging properties of the Gas Pixel Detector as a focal plane polarimeter
X-rays are particularly suited to probe the physics of extreme objects.
However, despite the enormous improvements of X-ray Astronomy in imaging,
spectroscopy and timing, polarimetry remains largely unexplored. We propose the
photoelectric polarimeter Gas Pixel Detector (GPD) as an instrument candidate
to fill the gap of more than thirty years of lack of measurements. The GPD, in
the focus of a telescope, will increase the sensitivity of orders of magnitude.
Moreover, since it can measure the energy, the position, the arrival time and
the polarization angle of every single photon, allows to perform polarimetry of
subsets of data singled out from the spectrum, the light curve or the image of
source. The GPD has an intrinsic very fine imaging capability and in this work
we report on the calibration campaign carried out in 2012 at the PANTER X-ray
test facility of the Max-Planck-Institut f\"ur extraterrestrische Physik of
Garching (Germany) in which, for the first time, we coupled it to a JET-X
optics module with a focal length of 3.5 m and an angular resolution of 18
arcsec at 4.5 keV. This configuration was proposed in 2012 aboard the X-ray
Imaging Polarimetry Explorer (XIPE) in response to the ESA call for a small
mission. We derived the imaging and polarimetric performance for extended
sources like Pulsar Wind Nebulae and Supernova Remnants as case studies for the
XIPE configuration, discussing also possible improvements by coupling the
detector with advanced optics, having finer angular resolution and larger
effective area, to study with more details extended objects.Comment: Accepted for publication in The Astrophysical Journal Supplemen
Rank-(n â 1) convexity and quasiconvexity for divergence free fields
The CAST experiment at CERN (European Organization of Nuclear Research)
searches for axions from the sun. The axion is a pseudoscalar particle that was
motivated by theory thirty years ago, with the intention to solve the strong CP
problem. Together with the neutralino, the axion is one of the most promising
dark matter candidates. The CAST experiment has been taking data during the
last two years, setting an upper limit on the coupling of axions to photons
more restrictive than from any other solar axion search in the mass range below
0.1 eV. In 2005 CAST will enter a new experimental phase extending the
sensitivity of the experiment to higher axion masses. The CAST experiment
strongly profits from technology developed for high energy physics and for
X-ray astronomy: A superconducting prototype LHC magnet is used to convert
potential axions to detectable X-rays in the 1-10 keV range via the inverse
Primakoff effect. The most sensitive detector system of CAST is a spin-off from
space technology, a Wolter I type X-ray optics in combination with a prototype
pn-CCD developed for ESA's XMM-Newton mission. As in other rare event searches,
background suppression and a thorough shielding concept is essential to improve
the sensitivity of the experiment to the best possible. In this context CAST
offers the opportunity to study the background of pn-CCDs and its long term
behavior in a terrestrial environment with possible implications for future
space applications. We will present a systematic study of the detector
background of the pn-CCD of CAST based on the data acquired since 2002
including preliminary results of our background simulations.Comment: 11 pages, 8 figures, to appear in Proc. SPIE 5898, UV, X-Ray, and
Gamma-Ray Space Instrumentation for Astronomy XI
Recommended from our members
Use of the ECL-CAMAC trigger processor system for recoil missing mass triggers at the Tagged Photon Spectrometer at Fermilab
A trigger processor in operation since May 1980 at the Tagged Photon Spectrometer at Fermilab will be described. The processor, based on the Fermilab ECL-CAMAC system, allows fast selection of high mass diffractive events from the total hadronic cross section. Data from a recoil detector, consisting of 3 wire chambers and 4 layers of scintillator concentric about a 1.5 m liquid hydrogen target, is digitized and presented to the processor within 3 sec. From the chamber data are found the vertices and angles of all recoiling tracks
First Light Measurements of Capella with the Low Energy Transmission Grating Spectrometer aboard the Chandra X-ray Observatory
We present the first X-ray spectrum obtained by the Low Energy Transmission
Grating Spectrometer (LETGS) aboard the Chandra X-ray Observatory. The spectrum
is of Capella and covers a wavelength range of 5-175 A (2.5-0.07 keV). The
measured wavelength resolution, which is in good agreement with ground
calibration, is 0.06 A (FWHM). Although in-flight
calibration of the LETGS is in progress, the high spectral resolution and
unique wavelength coverage of the LETGS are well demonstrated by the results
from Capella, a coronal source rich in spectral emission lines. While the
primary purpose of this letter is to demonstrate the spectroscopic potential of
the LETGS, we also briefly present some preliminary astrophysical results. We
discuss plasma parameters derived from line ratios in narrow spectral bands,
such as the electron density diagnostics of the He-like triplets of carbon,
nitrogen, and oxygen, as well as resonance scattering of the strong Fe XVII
line at 15.014 A.Comment: 4 pages (ApJ letter LaTeX), 2 PostScript figures, accepted for
publication in ApJ Letters, 200
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