The state of hyperlocal community news in the UK: findings from a survey of practitioners

Findings from a survey of practitioner

Can quantum gas microscopes directly image exotic glassy phases?

With the advent of spatially resolved fluorescence imaging in quantum gas microscopes (see e.g. [1]), it is now possible to directly image glassy phases and probe the local effects of disorder in a highly controllable setup. Here we present numerical calculations using a spatially resolved local mean-field theory, show that it captures the essential physics of the disordered system, and use it to simulate the density distributions seen in single-shot fluorescence microscopy [2]. From these simulated images we extract local properties of the phases which are measurable by a quantum gas microscope and show that unambiguous detection of the Bose glass is possible. In particular, we show that experimental determination of the Edwards-Anderson order parameter is possible in a strongly correlated quantum system using existing experiments. We also suggest modifications to the experiments by using spatial light modulators (see [3] and references therein) to tailor the lattice, which will allow further properties of the Bose glass to be measured. References: [1] E Haller, et al., "Single-atom imaging of fermions in a quantum-gas microscope" Nature Physics 11, 738 (2015) [2] S J Thomson, et al., "Measuring the Edwards-Anderson order parameter of the Bose glass: A quantum gas microscope approach" Phys. Rev. A 94, 051601(R) (2016) [3] F Buccheri, et al., "Holographic optical traps for atom-based topological Kondo devices" New J. Phys. 18, 075012 (2016)PostprintNon peer reviewe

Measuring the Edwards-Anderson order parameter of the Bose glass : a quantum gas microscope approach

We thank D Cassettari, A Daley, S Denny, J Keeling, P Kirton and A Trombettoni for insightful discussions and assistance. Computations were performed on the EPSRC CDT Computer Cluster and the University of St Andrews School of Physics & Astronomy computer cluster. SJT acknowledges studentship funding from EPSRC under grant no. EP/G03673X/1. GDB acknowledges support from the Leverhulme Trust RPG-2013-074.With the advent of spatially resolved fluorescence imaging in quantum gas microscopes, it is now possible to directly image glassy phases and probe the local effects of disorder in a highly controllable setup. Here we present numerical calculations using a spatially resolved local mean-field theory, show that it captures the essential physics of the disordered system and use it to simulate the density distributions seen in single-shot fluorescence microscopy. From these simulated images we extract local properties of the phases which are measurable by a quantum gas microscope and show that unambiguous detection of the Bose glass is possible. In particular, we show that experimental determination of the Edwards-Anderson order parameter is possible in a strongly correlated quantum system using existing experiments. We also suggest modifications to the experiments which will allow further properties of the Bose glass to be measured.PostprintPeer reviewe

The neurobiology of central sensitization

Central sensitization refers to the amplification of pain by central nervous system mechanisms. Classically described as a consequence of ongoing nociceptive input, it is increasingly recognized that central sensitization also occurs independent of peripheral injury or inflammation. Features of central sensitization have been identified in nearly all chronic pain conditions, and it is considered the primary underlying cause of pain in conditions such as fibromyalgia. Central sensitization is characterized in these conditions by widespread pain and multisite hyperalgesia/allodynia. Co‐occurring symptoms include fatigue, mood and cognitive problems, sleep disturbances, and multisensory hypersensitivity. Individuals with central sensitization often report previous exposure to psychosocial or physical stressors, and a higher personal lifetime and family history of pain, with the latter findings supported by genetic studies. Neuroimaging studies of central sensitization show evidence of: changes in brain gray matter in pain processing regions; neurochemical imbalances; and altered resting brain‐network connectivity between pronociceptive and antinociceptive brain areas. Immune system abnormalities have also been demonstrated in individuals with central sensitization. The recognition of central sensitization, and whether it is being driven by ongoing nociceptive input or it is occurring in the absence of a peripheral driver, is critical for effective pain management.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144668/1/jabr12137.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144668/2/jabr12137_am.pd

Volatile organic compounds associated with neonectria ditissima infection in apples (Malus pumila cv Gala)

Postharvest diseases in apples during long term storage result in loss and waste. This is mainly caused by fungal pathogens. Fungal contamination and rot can change some of the volatile organic compounds (VOC) emitted by apple fruits. In this study, disease free Gala apples were inoculated with Neonectria ditissima. The aim was to identify VOCs associated with N. ditissima infection in gala apples. The inoculated apples were placed in 5L glass flask, sealed, and incubated at 20oC for one hour after which a charcoal filtered airflow of 1 L/min was maintained for one hour through the Volatile Capture Trap (VCT) with volatile emissions captured on a porapak-Q absorbent filter. Captured volatiles were eluted using 1 mL of dichloromethane (DCM) into a standard Agilent 1.5 mL HPLC vial. Eluted volatiles were analysed using Gas Chromatography coupled with Mass Spectrometry (GC/MS). Volatiles were capture in three replicates for both inoculated and healthy control groups at 2 days, 8 days, 14 days, 21 days, 28 days, 35 days, and 42 days post-inoculation. The N. ditissima discriminatory volatile were identified/discriminated qualitatively based on the unique volatile compounds detected and quantitatively based on variation in peak area of certain combinations of volatile compounds. Some of the discriminatory volatiles such as dodecyl hexanoate, 9-decen-1-yl hexanoate, hexyl butanoate and pentyl acetate were detected in the early stages of the infection. Styrene, terpinene-4-ol, ethyl hexanoate, ethyl butanoate, ethyl pentanoate and 2-methylpentyl formate constituted the main VOCs emitted during apple fruit decay. Other compounds such as alpha Farnesene and hexyl acetate were common to both healthy and inoculated apples but the peak areas in the healthy apples were well above the peak areas in the inoculated apples. However, these compounds expressed a decline in peak area over time. Apples are stored commercially in sealed stores for months making visual observation for early detection of disease almost impossible. Disease of stored apples are most times only detected at advanced stages when it has become nearly impossible to prevent losses. These discriminatory volatile metabolites detected at early stages of infection are important for early non-visual detection of N. ditissima in stored apples. Further research is recommended to the use of these compounds in early detection of the disease caused by N. ditissima

Progress towards identification of a pheromone of the asparagus beetle, Crioceris asparagi (Coleoptera; Chrysomelidae)

The asparagus beetle, Crioceris asparagi L. (Coleoptera; Chrysomelidae) has become a widespread pest of asparagus, Asparagus officinalis (asparagus). There are no known attractants for C. asparagi and much of its biology is unknown. Volatiles were collected from individual beetles and a candidate pheromone component was detected in collections from some individuals of the first generation but not the second. This compound elicited an electroantennographic response from C. asparagi and the mass spectrum indicated it is a 19-carbon hydrocarbon with four double bonds. Complete structural elucidation and synthesis are in progress

Mining the untapped chemical potential of entomopathogenic fungi

• Given the immense fungal biodiversity (with an estimated 2.2 to 3.8 million species of fungi worldwide [1]) and broad range of fungal habitats, fungi are one of the best sources of natural bioactive compounds, with huge industrial and medicinal potential. • Entomopathogenic fungi (EPF) are fungi that infect and kill arthropods. • EPF produce unique secondary metabolites and rapidly adjust their metabolic outputs in response to changes in environmental conditions. [2] These metabolites are usually produced transiently, in low quantities or not at all under laboratory conditions. • With the market for commercial EPF biopesticides growing considerably in recent years, EPF secondary metabolites present an understudied and biotechnologically valuable opportunity for the pharmaceutical and agricultural industries. PROJECT AIM: Develop liquid culturing techniques of EPF to optimise metabolite production that facilitates structural elucidation and biological activity testing

Identification of components of the aggregation pheromone of the Guam strain of coconut rhinoceros beetle, Oryctes rhinoceros, and determination of stereochemistry

The coconut rhinoceros beetle, Oryctes rhinoceros (Linnaeus 1758) (Coleoptera: Scarabaeidae: Dynastinae) (CRB), is endemic to tropical Asia where it damages both coconut and oil palm. A new invasion by CRB occurred on Guam in 2007 and eradication attempts failed using commonly applied Oryctes rhinoceros nudivirus (OrNV) isolates. This and subsequent invasive outbreaks were found to have been caused by a previously unrecognized haplotype, CRB-G, which appeared to be tolerant to OrNV. The male-produced aggregation pheromone of the endemic, susceptible strain of O. rhinoceros (CRB-S) was previously identified as ethyl 4-methyloctanoate. Following reports from growers that commercial lures containing this compound were not attractive to CRB-G, the aim of this work was to identify the pheromone of CRB-G. Initial collections of volatiles from virgin male and female CRB-G adults from the Solomon Islands failed to show any male- or female-specific compounds as candidate pheromone components. Only after five months were significant quantities of ethyl 4-methyloctanoate and 4-methyloctanoic acid produced by males but not by females. No other male-specific compounds could be detected, in particular methyl 4-methyloctanoate, 4-methyl-1-octanol, or 4-methyl-1-octyl acetate, compounds identified in volatiles from some other species of Oryctes. Ethyl 4-methyloctanoate elicited a strong electroantennogram response from both male and female CRB-G, but these other compounds, including 4-methyloctanoic acid, did not. The enantiomers of ethyl 4-methyloctanoate and 4-methyloctanoic acid were conveniently prepared by enzymatic resolution of the commercially-available acid, and the enantiomers of the acid, but not the ester, could be separated by gas chromatography on an enantioselective cyclodextrin phase. Using this approach, both ethyl 4-methyloctanoate and 4-methyloctanoic acid produced by male CRB-G were shown to be exclusively the (R)-enantiomers whereas previous reports had suggested male O. rhinoceros produced the (S)-enantiomers. However, re-examination of the ester and acid produced by male CRB-S from Papua New Guinea showed that these were also the (R)-enantiomers. In field trapping experiments carried out in the Solomon Islands, both racemic and ethyl (R)-4-methyloctanoate were highly attractive to both male and female CRB-G beetles. The (S)-enantiomer and the corresponding acids were only weakly attractive. The addition of racemic 4-methyloctanoic acid to ethyl 4-methyloctanoate did significantly increase attractiveness, but the addition of (R)- or (S)-4-methyloctanoic acid to the corresponding ethyl esters did not. Possible reasons for the difference in assignment of configuration of the components of the CRB pheromone are discussed along with the practical implications of these results

Developing a semiochemical baited “smart” trap to monitor vine weevil (Otiorhynchus sulcatus)

Conference poster - No Abstract available

Identification of the aggregation pheromone of the invasive guam strain of coconut rhinoceros beetle, Oryctes rhinoceros, and determination of stereochemistry

The coconut rhinoceros beetle, Oryctes rhinoceros (CRB), is a major pest of coconut and oil palm that has been effectively controlled by the Oryctes rhinoceros nudivirus for over 30 years. A new haplotype, CRB-G, that is not controlled by the virus appeared in Guam in 2007 and has since spread to other countries in the Region. There has been much research on alternative methods to control CRB-G, and there have been reports that it does not respond to the well-established aggregation pheromone of CRB. We found that the male CRB-G beetles produce ethyl 4-methyloctanoate and 4-methyloctanoic acid in 4:1 ratio, essentially as reported for CRB previously by Hallett et al. (1995). The enantiomers of these compounds were synthesised by enzymatic resolution and both the male-produced compounds were shown to be (R)-enantiomers. Hallet et al. (1995) reported that CRB produced the (S)-enantiomers on the basis of field studies, but re-examination of the pheromone produced by CRB beetles confirmed that they also produced the (R)-enantiomers. Electroantennogram (EAG) responses to natural volatile collections from Oryctes and to the synthetic compounds indicated that both male and female beetles respond to the ester but not to the acid. EAG responses were recorded to both enantiomers, but responses to the ethyl (R)-4-methyloctanoate were consistently greater than those to the (S)-enantiomer. In field testing in Papua New Guinea, ethyl (R)-4-methyloctanoate was attractive to both male and female CRB-G beetles and significantly more attractive than the (S)-enantiomer. The racemic ester was as attractive as the (R)-enantiomer, and addition of (R)-4-methyloctanoic acid gave a marginal increase in attractiveness of the lure. Thus CRB-G beetles produce the same pheromone as CRB, although the enantiomeric composition of this was previously wrongly assigned. Both male and female CRB-G are attracted by racemic ethyl 4-methyloctanoate in the field, so that the same lures can be used for monitoring and control of CRB-G as for CRB