Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro

Propionibacterium acnes plays an important role in inducing skin inflammatory mediators and comedogenic acne. This study aimed to evaluate the antimicrobial effect of Strychnos ligustrina Blume leaf extract against P. acnes. This study was an in-vitro experiment with a completely randomized design (CRD) testing five concentrations of ethanolic extract of S. ligustrina leaves. The control treatment was the antibiotic Kindamicin. The anti-bacterial evaluation of the extract was carried out on Mueller-Hinton agar media by using the diffusion method. The inhibition zone was assessed according to NCCLS (National Committee for Clinical Laboratory Standards). Analysis and interpretation of the results used 95% ANOVA (α: 0.05). The results of the study showed that S. ligustrina leaf extract inhibited the bacterial growth of P. acnes. The minimum concentration of the extract that inhibited the growth of P. acne was 25% with a diameter inhibition zone of 4.13 ± 0.13 mm, while the highest concentration of the undiluted extract was 7.88 ± 0.67 mm. Compared to the standard antibiotic Kindamycin (positive control), the ethanolic leaf extract of S. ligustrina was less effective having an inhibition zone of 24.81 ± 0.54 mm. The study suggests that S. ligustrina leaf extract has antimicrobial effects on P. acnes and may be further studied as an herbal remedy for acne

Identification of fungal pathogens to control postharvest passion fruit (Passiflora edulis) decays and multi-omics comparative pathway analysis reveals purple is more resistant to pathogens than a yellow cultivar

Production of passion fruit (Passiflora edulis) is restricted by postharvest decay, which limits the storage period. We isolated, identified, and characterized fungal pathogens causing decay in two passion fruit cultivars during two fruit seasons in China. Morphological characteristics and nucleotide sequences of ITS-rDNA regions identified eighteen isolates, which were pathogenic on yellow and purple fruit. Fusarium kyushuense, Fusarium concentricum, Colletotrichum truncatum, and Alternaria alternata were the most aggressive species. Visible inspections and comparative analysis of the disease incidences demonstrated that wounded and non-wounded yellow fruit were more susceptible to the pathogens than the purple fruit. Purple cultivar showed higher expression levels of defense-related genes through expression and metabolic profiling, as well as significantly higher levels of their biosynthesis pathways. We also found fungi with potential beneficial features for the quality of fruits. Our transcriptomic and metabolomics data provide a basis to identify potential targets to improve the pathogen resistance of the susceptible yellow cultivar. The identified fungi and affected features of the fruit of both cultivars provide important information for the control of pathogens in passion fruit industry and postharvest storage

Leaf extract of

Propionibacterium acnes plays an important role in inducing skin inflammatory mediators and comedogenic acne. This study aimed to evaluate the antimicrobial effect of Strychnos ligustrina Blume leaf extract against P. acnes. This study was an in-vitro experiment with a completely randomized design (CRD) testing five concentrations of ethanolic extract of S. ligustrina leaves. The control treatment was the antibiotic Kindamicin. The anti-bacterial evaluation of the extract was carried out on Mueller-Hinton agar media by using the diffusion method. The inhibition zone was assessed according to NCCLS (National Committee for Clinical Laboratory Standards). Analysis and interpretation of the results used 95% ANOVA (α: 0.05). The results of the study showed that S. ligustrina leaf extract inhibited the bacterial growth of P. acnes. The minimum concentration of the extract that inhibited the growth of P. acne was 25% with a diameter inhibition zone of 4.13 ± 0.13 mm, while the highest concentration of the undiluted extract was 7.88 ± 0.67 mm. Compared to the standard antibiotic Kindamycin (positive control), the ethanolic leaf extract of S. ligustrina was less effective having an inhibition zone of 24.81 ± 0.54 mm. The study suggests that S. ligustrina leaf extract has antimicrobial effects on P. acnes and may be further studied as an herbal remedy for acne