1,164 research outputs found
Characterization, cloning and immunogenicity of antigens released by lung-stage larvae of Schistosoma mansoni
Lung-stage schistosomula are the target of protective immunity in mice vaccinated with attenuated cercariae of Schistosoma mansoni. Therefore, proteins present at this developmental stage, and in particular those which are secreted, are a potential source of novel vaccine candidates. However, little information is available about such molecules. Here we describe the cDNA clones identified by screening expression libraries with serum raised against proteins released by lung-stage schistosomula. In total, 11 different cDNA species were identified, 6 of which have been described previously in S. mansoni; these included fructose 1,6-bisphosphate aldolase and Sm21.7 which together accounted for two-thirds of all positive clones. Of the 5 newly described schistosome genes, 1 cDNA had a high degree of homology to the s5a subunit of 26S proteasomes, most significant being with the human protein. The remaining 4 clones showed no significant homologies to any genes sequenced previously. Fructose 1,6-bisphosphate aldolase, Sm21.7, the proteasome homologue and 1 unknown clone (A26) have been expressed in a bacterial expression system and serum produced against each recombinant protein. Immunolocalization showed fructose 1,6-bisphosphate aldolase, Sm21.7 and the proteasome homologue to be most abundant in muscle cells whilst clone A26 was distributed throughout many tissues, but was most abundant in the tegument. Analysis of the cellular immune responses of vaccinated mice showed 3 of the 4 expressed clones to be highly immunogenic, inducing the secretion of large quantities of the Th1-type cytokine interferon gamma
All carrot â no stick: an alternative award framework to enhance âinternationalâ studentsâ sense of belonging and engagement in the extracurricular
A sense of belonging and feeling at home at their institution are key factors in student success at university. Engaging with extracurricular activities is part of this dynamic, and an area in which âinternationalâ students face additional barriers. These include institutional and psychological barriers to belonging, resulting in a shortage of meaningful opportunities to belong and a lack of motivation to take part. Increased belonging uncertainty and negative stereotype threat are among the potential concerns for such students. In response, we have developed the Student Extracurricular Engagement Award for pre-sessional undergraduate students at the University of Leeds Language Centre, UK. This scheme draws on theoretical approaches related to co-construction, participation, identity, opportunity and motivation. This innovative educational intervention seeks to address the issues affecting belonging by providing encouragement and guidance in engaging with the universityâs extracurricular activities. By validating activities in areas other than academic language and literacies, the award raises student awareness of the value of their successes in those areas. This more holistic and rhizomatic approach to student learning is argued to bring a greater sense of belonging to âinternationalâ students
Infrared spectroscopy of cataclysmic variables: III. Dwarf novae below the period gap and novalike variables
We present K-band spectra of the short-period dwarf novae YZ Cnc, LY Hya, BK
Lyn, T Leo, SW UMa and WZ Sge, the novalike variables DW UMa, V1315 Aql, RW
Tri, VY Scl, UU Aqr and GP Com, and a series of field dwarf stars with spectral
types ranging from K2-M6.
The spectra of the dwarf novae are dominated by emission lines of HI and HeI.
The large velocity and equivalent widths of these lines, in conjunction with
the fact that the lines are double-peaked in the highest inclination systems,
indicate an accretion disc origin. In the case of YZ Cnc and T Leo, for which
we obtained time-resolved data covering a complete orbital cycle, the emission
lines show modulations in their equivalent widths which are most probably
associated with the bright spot (the region where the gas stream collides with
the accretion disc). There are no clear detections of the secondary star in any
of the dwarf novae below the period gap, yielding upper limits of 10-30% for
the contribution of the secondary star to the observed K-band flux. In
conjunction with the K-band magnitudes of the dwarf novae, we use the derived
secondary star contributions to calculate lower limits to the distances to
these systems.
The spectra of the novalike variables are dominated by broad, single-peaked
emission lines of HI and HeI - even the eclipsing systems we observed do not
show the double-peaked profiles predicted by standard accretion disc theory.
With the exception of RW Tri, which exhibits NaI, CaI and 12CO absorption
features consistent with a M0V secondary contributing 65% of the observed
K-band flux, we find no evidence for the secondary star in any of the novalike
variables. The implications of this result are discussed.Comment: 13 pages, 5 figures, to appear in MNRA
Intramedullary Spinal Cord Metastases and Radiation Therapy: A Case Report
Intramedullary spinal cord metastases (ISCM) are a clinically rare, although devastating, complication of disseminated cancer. These lesions have been reported to originate from many types of solid tumors, although primary lung carcinoma, particularly small cell, is the most common etiology. These metastases, which can occur anywhere along the spinal cord, often represent the end-stage of the disease process with limited survival outcomes.
Patients with ISCM may develop a variety of neurological deficits with treatment goals aimed at palliation. Different modalities of treatment have been found to preserve or restore ambulation and neurological function. The options for therapeutic intervention include surgical, chemotherapeutic, and radiation therapy. We describe a case of ISCM in a patient with disseminated small cell lung cancer with magnetic resonance imaging that illustrates a complete tumor response to radiation therap
Whiplash: diagnosis, treatment, and associated injuries
Study design Focused review of the current literature. Objective To identify and synthesize the most current data pertaining to the diagnosis and treatment of whiplash and whiplash-associated disorders (WAD), and to report on whiplash-related injuries. Methods A search of OVID Medline (1996âJanuary 2007) and the Cochrane database of systematic reviews was performed using the keywords whiplash and WAD. Articles under subheadings for pathology, diagnosis, treatment, and epidemiology were chosen for review after identification by the authors. Results A total of 485 articles in the English language literature were identified. Thirty-six articles pertained to the diagnosis, treatment, epidemiology of whiplash, and WAD, and were eligible for focused review. From these, 21 primary and 15 secondary sources were identified for full review. In addition, five articles were found that focused on whiplash associated cervical injuries. These five articles were also primary sources. Conclusions Whiplash is a common injury associated most often with motor vehicle accidents. It may present with a variety of clinical manifestations, collectively termed WAD. Whiplash is an important cause of chronic disability. Many controversies exist regarding the diagnosis and treatment of whiplash injuries. The multifactorial etiology, believed to underly whiplash injuries, make management highly variable between patients. Radiographic evidence of injury often cannot be identified in the acute phase. Recent studies suggest early mobilization may lead to improved outcomes. Ligamentous and bony injuries may go undetected at initial presentation leading to delayed diagnosis and inappropriate therapies
A second independent audit of electroconvulsive therapy in England, 2019: Usage, demographics, consent, and adherence to guidelines and legislation
Objectives. To assess progress towards improving the administering of Electroconvulsive therapy (ECT) in England since an audit covering 2011, 2013 and 2015. The same information was gathered, for 2019, on usage, demographics, consent, and adherence to national guidelines and the Mental Health Act.
Design and Methods. Freedom of Information Act requests were sent to 56 National Health Service Trusts.
Results. Thirty-seven trusts (66%) provided data. The gradual decline in the use of ECT in England has levelled off at about 2,500 people per year. There was a 47-fold difference between the Trusts with the highest and lowest rates per capita. Most recipients are still women (67%), and over 60 (58%). Only one Trust could report how many people received psychological therapy prior to ECT, as required by government (N.I.C.E.) guidelines. More than a third of ECT (37%) is still given without consent, with 18% of Trusts non-compliant with legislation concerning second opinions. There were slight declines, compared to a previous audit, in the use of standardised depression scales, down to 30%, and standardised measures of cognitive dysfunction, down to 24%. Only six Trusts provided any data for positive outcomes; seven for adverse effects. None provided data on efficacy or adverse effects beyond end of treatment. Twelve used identical sentences to each other, verbatim, in response to one or more questions.
Conclusions. Given the apparent failure of current monitoring and accrediting ECT clinics in England, by the Royal College of Psychiatristsâ ECT Accreditation Service (ECTAS), an independent government sponsored review is urgently needed
UBVRI photopolarimetry of the long period eclipsing AM Herculis binary V1309
We report simultaneous UBVRI photo-polarimetric observations of the long
period (7.98 h) AM Her binary V1309 Ori. The length and shape of the eclipse
ingress and egress varies from night to night. We suggest this is due to the
variation in the brightness of the accretion stream. By comparing the phases of
circular polarization zero-crossovers with previous observations, we confirm
that V1309 Ori is well synchronized, and find an upper limit of 0.002 percent
for the difference between the spin and orbital periods. We model the
polarimetry data using a model consisting of two cyclotron emission regions at
almost diametrically opposite locations, and centered at colatitude 35 (deg)
and 145 (deg) on the surface of the white dwarf. We also present archive X-ray
observations which show that the negatively polarised accretion region is X-ray
bright.Comment: 11 pages, 12 figures (2 colour), Fig1 and Fig 4 are in lower
resolution than in original paper, accepted for publication in Monthly
Notices of the Royal Astronomical Societ
Deprescribing interventions and their impact on medication adherence in community-dwelling older adults with polypharmacy: a systematic review. Abstract only
- âŠ