Putting the Restroom Debate to Rest: Addressing Title IX and Equal Protection in G.G. ex rel. Grimm v. Gloucester County School Board

Courts, legislatures, and citizens have been debating whether transgender students should use the restroom that corresponds to their biological sex, or whether they can choose to use the facilities which align with their gender identity. The Fourth Circuit decided that Title IX required the latter in G.G. ex. rel. Grimm v. Gloucester County School Board, but that didn\u27t settle the issue. To complicate things, the Trump Administration revoked the guidance that the Fourth Circuit had relied on. This Note argues that a court should resolve this once and for all by finding that a school policy that requires students to use the restroom that matches their biological sex and disregards their gender identity is both a violation of Title IX and of the Equal Protection Clause

Do Repugnant Scents Increase Survival of Ground Nests? A Test with Artificial and Natural Duck Nests

Ground-nesting birds typically experience high predation rates on their nests, often by mammalian predators. As such, researchers and wildlife managers have employed numerous techniques to mitigate nest predation. We investigated the use of scents as repellents to deter predators from both artificial and natural ground nests. Survival rates of artificial nests did not differ among six groups of substances (Wald ?2 df = 5 = 4.53, P < 0.48); however the chronology of predation among groups differed. A commercial Coyote urine based deterrent (DEER-D-TERTM), human hair, and Worcestershire sauce were depredated faster than the control (F4,5 = 40.3, P < 0.001). Nest survival of natural nests differed among those groups tested (Wald ?2 df = 2 = 11.8, P < 0.005); the eight mothball treatment decreased survival (Wald ?2 df = 1 = 11.5, P < 0.005), which indicated that novel smells may attract predators or result in duck nest abandonment when coupled with natural duck scent. Chronologies of predation events among treatment groups were not different for natural nests (F2,3 = 1.9, P = 0.22). These findings indicate an interaction between novel scents and predator olfactory cues

Slow acid production by butter cultures

Butter cultures sometimes fail to develop acid at a normal rate, and such slow growth has probably been encountered wherever butter cultures are used. One characteristic of certain types of abnormally slow growth in butter cultures is the sudden manner in which the defect often occurs. A culture may appear satisfactory at the time it is used for inoculation and then fail to bring about the desired changes in the product being manufactured. Such an occurrence greatly interferes with general plant routine. If additional time is allowed for the formation of acid, a defective product may result. Sometimes acid development is so slow and the defects in the product being manufactured so serious that the finished material is unsalable. There are various causes of slow acid production by butter cultures. Some of these are easily determined by investigation of the methods used in propagation or by direct microscopic examination of the cultures. In other cases such methods fail to disclose the cause or causes of the abnormally slow growth, and the rather unusual aspects of such cases have motivated the work herein reported

Small General Aviation Airport Emergency Preparedness and the Perceived Risks of Very Light Jet Operations

Advances in aircraft design have facilitated to the development of relatively low cost, high performance, light weight jet aircraft known as very light jets (VLJs). The Federal Aviation Administration\u27s prediction of 4,500 VLJs flying by 2016 suggests that this concept may become a major contributing factor for gridlock in the National Airspace System WAS) (Robinson and Planzer, 2005). With this added burden, the need for effective emergency response at general aviation airports will continue to increase. This study investigated the level of emergency response preparedness of small general aviation airports in the state of Indiana that may service very light jets and measured attitudes of airport managers regarding the perceived risks of VLJ operations. Findings suggest that airport managers believe smaller airports are prepared to handle the growth in VLJ traffic and generally are not concerned with increasing the level of emergency response planning. This study concludes with recommendations for emergency enhancements at smaller airports not certified under Part 139

Electronic Energy Transfer to the S2 Level of the Acceptor in Functionalised Boron Dipyrromethene Dyes

Taking the high road: Highly efficient electronic energy transfer takes place from a set of appended aryl polycyclic hydrocarbons to an expanded boron dipyrromethene (Bodipy)-based dye (see figure) despite negligible spectral overlap with the lowest-energy excited state localised on the acceptor.A multi-component array has been constructed around an expanded boron dipyrromethene (Bodipy) dye that absorbs and emits in the far-red region. One of the appendages is a perylene-based moiety that is connected to the boron atom of the terminal Bodipy by a 1,4-diethynylphenylene connector. Despite the fact that there is almost negligible spectral overlap between fluorescence from the perylene unit and absorption by the Bodipy residue, electronic energy transfer is rapid and essentially quantitative. It is concluded that at least half of the photons absorbed by perylene are transferred to the upper-lying singlet excited state (S2) associated with the Bodipy-based acceptor. The second appendage is a pyrene unit that is covalently linked to fluorene, through an ethynylene spacer, and to the boron atom of the Bodipy terminus, through a 1,4-diethynylphenylene connector. Pyrene absorbs and emits at higher energy than perylene and there is strong spectral overlap with the Bodipy-based S2 state, and none with the corresponding S1 state. Electronic energy transfer is now very fast and exclusively to the S2 state of the acceptor. It is difficult to compute reasonable estimates for the rates of Coulombic energy transfer, because of uncertainties in the orientation factor, but the principle mechanism is believed to arise from electron exchange. Comparison with an earlier array built around a conventional Bodipy dye indicates that there are comparable electronic coupling matrix elements for the two systems. It is notable that pyrene is more strongly coupled to the Bodipy unit than perylene in both arrays. These new arrays function as highly effective solar concentrators

A diverse repertoire of human immunoglobulin variable genes in a chicken B cell line is generated by both gene conversion and somatic hypermutation

Chicken immune responses to human proteins are often more robust than rodent responses because of the phylogenetic relationship between the different species. For discovery of a diverse panel of unique therapeutic antibody candidates, chickens therefore represent an attractive host for human-derived targets. Recent advances in monoclonal antibody technology, specifically new methods for the molecular cloning of antibody genes directly from primary B cells, has ushered in a new era of generating monoclonal antibodies from non-traditional host animals that were previously inaccessible through hybridoma technology. However, such monoclonals still require post-discovery humanization in order to be developed as therapeutics. To obviate the need for humanization, a modified strain of chickens could be engineered to express a human-sequence immunoglobulin variable region repertoire. Here, human variable genes introduced into the chicken immunoglobulin loci through gene targeting were evaluated for their ability to be recognized and diversified by the native chicken recombination machinery that is present in the B-lineage cell line DT40. After expansion in culture the DT40 population accumulated genetic mutants that were detected via deep sequencing. Bioinformatic analysis revealed that the human targeted constructs are performing as expected in the cell culture system, and provide a measure of confidence that they will be functional in transgenic animals