913 research outputs found

    Structure of d(TGCGCA)(2) and a comparison with other DNA Hexamers

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    The X-ray crystal structure of d(TGCGCA)(2) has been determined at 120 K to a resolution of 1.3 Angstrom. Hexamer duplexes, in the Z-DNA conformation, pack in an arrangement similar to the 'pure spermine form' [Egli et al. (1991). Biochemistry, 30, 11388-11402] but with significantly different cell dimensions. The phosphate backbone exists in two equally populated discrete conformations at one nucleotide step, around phosphate 11. The structure contains two ordered cobalt hexammine molecules which have roles in stabilization of both the Z-DNA conformation of the duplex and in crystal packing. A comparison of d(TGCGCA)(2) with other Z-DNA hexamer structures available in the Nucleic Acid Database illustrates the elusive nature of crystal packing. A review of the interactions with the metal cations Na+, Mg2+ and Co3+ reveals a relatively small proportion of phosphate binding and that close contacts between metal ions are common. A prediction of the water structure is compared with the observed pattern in the reported structure

    Organic carbon compounds associated with deep soil carbon stores

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    Aims Organic carbon has been reported in deep regolithic profiles to depths of tens of metres, but the composition of the carbon compounds is unknown. Methods Residual carbon in the form of non-volatile low molecular weight compounds (LMWC) was characterised in three deep soil profiles to a depth of 19 m under farmland in south-western Australia following extraction with ethyl acetate and analysis by GC/MS. Pyrolysis and off-line thermochemolysis were used to characterise macromolecular organic carbon (MOC) to a depth of 29 m at a fourth site. Results Three compound classes occurred across the three different field locations: (1) terpenes, (2) fatty acids, amides and alcohols, and (3) plant steroids; indicating the influence of input of the past and present vegetation. Compounds related to fatty acids were the predominant residual carbon species in deep soils, and may be derived from plants and microorganisms. Biomarkers such as lignin, polysaccharides, proteins and terpenes at 0–0.1 m implied influences of vegetation, fire events and microorganisms. Pyrolysis found that polysaccharides were distributed mainly from 0 to 0.1 m, while aromatic compounds were consistently detected down to 29 m. Conclusions Carbon was stabilised in the form of aromatic compounds in deep soil, whereas other carbon sources such as cellulose, chitin, and N-containing compounds were confined to the surface soil. LMWC (Z)-docos-13-enamide and bis(6-methylheptyl) phthalate, were the main components throughout the soil profiles representing 53–81% of the LMWC, and were a greater proportion of the organic matter at depths of 18–19 m

    Responses of streamflow to vegetation and climate change in southwestern Australia

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    Southwestern Australia has experienced recent climate change, with an increase in air temperature of 0.6°C and a reduction in mean annual precipitation of -15% since 1970. Along with the warming and drying trends, dramatic declines of streamflow have occurred across the region. However, both forest mortality and an increase in leaf area index have been observed in the southwestern forest, suggesting varied responses of vegetation to climate change. In this study, 30 catchments were analyzed using the Mann-Kendall trend test, Pettitt’s change point test and the theoretical framework of the Budyko curve to study the rainfall-runoff relationship change, and effects of climate and land cover change on streamflow. A declining trend and relatively consistent change point (2000) of streamflow were found in most catchments, with 14 catchments showing significant declines (p < 0.05, -68.1% to -35.6%) over 1970-2000 and 2001-2015. Most of the catchments have been shifting towards a more water-limited climate condition since 2000. For the period of 1970 to 2015, the dynamic of vegetation attributes (land cover/use change and growth of vegetation) dominated the decrease of streamflow in about half the study catchments. In general, a coequal role of climate and vegetation on the decline in streamflow was found in the study, suggesting the importance of vegetation management on future water management and production. Precipitation is predicted to decline in the future; therefore, some forest management intervention is required to maintain forest growth and water supply in the southwest of Australia

    Phase Farming with Trees: A report for the RIRDC/LWRRDC/FWPRDC Joint Venture Agroforestry Program

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    A scoping study was undertaken to determine the economic and biophysical feasibility of a proposal to research a system of phase farming with trees (PFT) in medium to low (300-600 mm) rainfall areas of southern Australia. This system is designed to use trees grown in very short term rotations (3-5 years) to rapidly de-water farming catchments, at risk of salinity, by depleting unsaturated stored soil water and reducing recharge while producing utilizable products. If feasible, the system will utilize a resource that is currently contributing to environmental problems while building more sustainable agricultural systems. Potential benefits include decreased salinization, improved farm cash flows, improved soil structure and acting as a disease and weed break..

    Defining biodiverse reforestation: Why it matters for climate change mitigation and biodiversity

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    Reforestation to capture and store atmospheric carbon is increasingly championed as a climate change mitigation policy response. Reforestation plantings have the potential to provide conservation co-benefits when diverse mixtures of native species are planted, and there are growing attempts to monetise biodiversity benefits from carbon reforestation projects, particularly within emerging carbon markets. But what is meant by ‘biodiverse’ across different stakeholders and groups implementing and overseeing these projects and how do these perceptions compare with long-standing scientific definitions? Here, we discuss approaches to, and definitions of, biodiversity in the context of reforestation for carbon sequestration. Our aim is to review how the concept of biodiversity is defined and applied among stakeholders (e.g., governments, carbon certifiers and farmers) and rights holders (i.e., First Nations people) engaging in reforestation, and to identify best-practice methods for restoring biodiversity in these projects. We find that some stakeholders have a vague understanding of diversity across varying levels of biological organisation (genes to ecosystems). While most understand that biodiversity underpins ecosystem functions and services, many stakeholders may not appreciate the difficulties of restoring biodiversity akin to reference ecosystems. Consequently, biodiversity goals are rarely explicit, and project goals may never be achieved because the levels of restored biodiversity are inadequate to support functional ecosystems and desired ecosystem services. We suggest there is significant value in integrating biodiversity objectives into reforestation projects and setting specific restoration goals with transparent reporting outcomes will pave the way for ensuring reforestation projects have meaningful outcomes for biodiversity, and legitimate incentive payments for biodiversity and natural capital accounting

    Seasonal timing for estimating carbon mitigation in revegetation of abandoned agricultural land with high spatial resolution remote sensing

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    Dryland salinity is a major land management issue globally, and results in the abandonment of farmland. Revegetation with halophytic shrub species such as Atriplex nummularia for carbon mitigation may be a viable option but to generate carbon credits ongoing monitoring and verification is required. This study investigated the utility of high-resolution airborne images (Digital Multi Spectral Imagery (DMSI)) obtained in two seasons to estimate carbon stocks at the plant- and stand-scale. Pixel-scale vegetation indices, sub-pixel fractional green vegetation cover for individual plants, and estimates of the fractional coverage of the grazing plants within entire plots, were extracted from the high-resolution images. Carbon stocks were correlated with both canopy coverage (R2: 0.76-0.89) and spectral-based vegetation indices (R2: 0.77-0.89) with or without the use of the near-infrared spectral band. Indices derived from the dry season image showed a stronger correlation with field measurements of carbon than those derived from the green season image. These results show that in semi-arid environments it is better to estimate saltbush biomass with remote sensing data in the dry season to exclude the effect of pasture, even without the refinement provided by a vegetation classification. The approach of using canopy cover to refine estimates of carbon yield has broader application in shrublands and woodlands

    Mitigation of carbon using Atriplex nummularia revegetation

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    The use of abandoned or marginally productive land to mitigate greenhouse gas emissions may avoid competition with food and water production. Atriplex nummularia Lindl. is a perennial shrub commonly established for livestock forage on saline land, however, its potential for carbon mitigation has not been systematically evaluated. Similarly, although revegetation is an allowable activity to mitigate carbon within Article 3.4 of the United Nations Framework Convention on Climate Change's Kyoto Protocol, there is a paucity of information on rates of carbon mitigation in soils and biomass through this mechanism. For six sites where A. nummularia had been established across southern Australia four were used to assess changes in soil carbon storage and four were used to develop biomass carbon sequestration estimates. A generalised allometric equation for above and below ground biomass was developed, with a simple crown volume index explaining 81% of the variation in total biomass. There were no significant differences in soil organic carbon storage to 0.3 m or 2 m depth compared to existing agricultural land-use. Between 2.2 and 8.3 Mg C ha−1 or 0.2–0.6 Mg C ha−1 yr−1 was sequestered in above and below ground biomass and this translates to potential total sequestration of 1.1–3.6 Tg C yr−1 on saline land across Australia. Carbon income and forage grazing may thus provide a means to finance the stabilization of compromised land
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