2,074 research outputs found
The GEOS-2 heat pipe system and its performance in test and orbit
GEOS 2 satellite heat transfer pipe performance in test and orbi
Evaluation report for secondary spacecraft cells - Cycle life test Annual report
Life cycle test evaluation of secondary spacecraft cell
Gender division of housework during the COVID-19 pandemic: Temporary shocks or durable change?
The accumulation of economic disadvantage:The Influence of Childbirth and Divorce on the Income and Poverty Risk of Single Mothers
Single mothers’ income in twelve rich nations:differences in disadvantage across the distribution
Regulation of the terminal steps in peptidoglycan biosynthesis in ether-treated cells of Escherichia coli
A crucial role of the mitochondrial protein import receptor MOM19 for the biogenesis of mitochondria
The novel genetic method of "sheltered RIP" (repeat induced point mutation) was used to generate a Neurospora crassa mutant in which MOM19, a component of the protein import machinery of the mitochondrial outer membrane, can be depleted. Deficiency in MOM19 resulted in a severe growth defect, but the cells remained viable. The number of mitochondrial profiles was not grossly changed, but mutant mitochondria were highly deficient in cristae membranes, cytochromes, and protein synthesis activity. Protein import into isolated mutant mitochondria was decreased by factors of 6 to 30 for most proteins from all suborganellar compartments. Proteins like the ADP/ATP carrier, MOM19, and cytochrome c, whose import into wild-type mitochondria occurs independently of MOM19 became imported normally showing that the reduced import activities are solely caused by a lack of MOM19. Depletion of MOM19 reveals a close functional relationship between MOM19 and MOM22, since loss of MOM19 led to decreased levels of MOM22 and reduced protein import through MOM22. Furthermore, MOM72 does not function as a general backup receptor for MOM19 suggesting that these two proteins have distinct precursor specificities. These findings demonstrate that the import receptor MOM19 fulfills an important role in the biogenesis of mitochondria and that it is essential for the formation of mitochondria competent in respiration and phosphorylation
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