Additional file 8: Figure S7. of EPCR promotes breast cancer progression by altering SPOCK1/testican 1-mediated 3D growth

Cell growth kinetics of APC-stimulated breast cancer cell lines. A. MTS proliferation assay of cells stimulated with increasing doses of APC. Data were normalized with absorbance values from day 0. Each dot represents mean ± SD of six replicates. B. Percentage of cells in each phase of the cell cycle in control and 50 nM APC-stimulated cells for 24 and 48 h, in serum-free and 4% serum medium. C. Percentage of apoptotic cells in basal and staurosporine-induced conditions, measured by annexin-V binding flow cytometry assay. Cell lines are MDA-MB-231,1833, BT-549, and ANV5, from the left to the right, in all figure sections. (PPTX 325 kb

"Ich bin ein Sachse, protestiere aber nicht, wenn mich ein bodenständiger Deutscher für einen Rumänen hält." : das (inter-)kulturelle Portrait Paul Schusters

This article is dedicated to the intercultural aspects of Paul Schuster’s stories (1930-2004), a German writer, born in Sibiu, regarded by German literary historians and criticists as one of the most talented prose writers descending from the small German cultural enclave of Transylvania. His work is thematically focused on events of the past century; The German minority he belongs to plays a decisive role, but also its cohabitation with different ethnic groups in Romania as well as the interethnic relations between them. Interculturality in Paul Schuster's stories is revealed on several levels: cultural exchanges between different ethnic groups, aspects of interethnic collaboration, imagology, linguistic interferences and translations from Romanian authors

Additional file 4: Figure S3. of EPCR promotes breast cancer progression by altering SPOCK1/testican 1-mediated 3D growth

Immunohistochemical analysis of several markers in control and EPCR-silenced size-matched mammary tumors resected at different time points. A. Representative images showing H&E staining (×2.5 magnification) and the immunohistochemical staining of Ki67, cleaved caspase-3, CD31, and F4/80 (×20 magnification) in formaldehyde-fixed tumors. Scale bars 80 μm (H&E) and 10 μm (Ki67, caspase-3, CD31, and F4/80). T. mass, tumor mass. T. border, tumor border. B. Quantification of the percentage of immunoreactive cells. Each dot represents one tumor. Data are mean ± SEM. ns means non-statistical significance. (PPTX 2780 kb

Additional file 3: Figure S2. of EPCR promotes breast cancer progression by altering SPOCK1/testican 1-mediated 3D growth

Effects of EPCR silencing in vitro and in vivo tumor growth in an orthotopic model. A. Western blot analysis of EPCR protein levels in human BT-549 (top) and murine ANV5 (bottom) cells transduced with a scramble shRNA (shControl) and shRNAs targeting human (shEPCR#1 and shEPCR#2 in BT-549) and murine (shEPCR#3 in ANV5) EPCR. β-tubulin was used as loading control. White line indicates that the membrane was cut. B. MTS in vitro proliferation assay of BT-549 (top) and ANV5 (bottom) cells. Data were normalized with absorbance values from day 0 and represent mean ± SD of six replicates. C. Percentage of BT549 (top) and ANV5 (bottom) cells in each phase of the cell cycle after maintaining cells in culture for 24 and 48 h. Sta, staurosporine. D. Percentage of apoptotic BT-549 (top) and ANV5 (bottom) cells in basal and staurosporine-induced conditions, measured by annexin-V binding flow cytometry assay. E. Quantification of spheres grown in 3D matrigel cultures. Data are mean ± SD of 8 replicates. Representative images at ×4 magnification. Scale bar 0.5 mm. F. Outline of the in vivo orthotopic experiment (n = 8 per group). G. Quantification of tumor volume at day 15 post-injection. H. Kaplan–Meier curves of resection-free survival. (PPTX 9850 kb