FT-IR spectroscopy of the major coat protein of M13 and Pf1 in the phage and reconstituted into phospholipid systems

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Vitamin D2 at high and low concentrations exert opposing effects on molecular order and dynamics of dipalmitoyl phosphatidylcholine membranes

Fourier Transform Infrared spectroscopic studies show that low concentrations of vitamin D2 (1 and 3 mol %) does not induce significant change in the overall shape of the thermotropic profile of dipalmitoyl phosphatidylcholine (DPPC) membrane. In contrast, at higher concentrations of vitamin D2 (9 and 12 mol %), the phase transition shifts to lower temperatures and a significant broadening in the phase transition curve is also observed. Low concentration of vitamin D2 decreases the frequency of the CH2 stretching mode, implying an ordering effect, whilst high concentration of vitamin D2 disorders the system. Furthermore, at low and high concentrations, vitamin D2 causes opposing effect on membrane dynamics. It decreases the bandwidth of the CH2 stretching modes at low concentrations while increasing it at high concentrations. We have also observed different actions of vitamin D2 at low and high concentrations in the deep interior and interfacial region of the membrane, by monitoring the frequency of the CH3 stretching band and C=O stretching bands, respectively

Infrared difference spectroscopy as a physical tool to study biomolecules

This brief review focuses on the information that can be obtained for biomolecules, especially proteins, by using the technique infrared-induced difference spectroscopy.SCOPUS: re.jinfo:eu-repo/semantics/publishe