349 research outputs found
Avicin D, a Plant Triterpenoid, Induces Cell Apoptosis by Recruitment of Fas and Downstream Signaling Molecules into Lipid Rafts
Avicins, a family of triterpene electrophiles originally identified as potent inhibitors of tumor cell growth, have been shown to be pleiotropic compounds that also possess antioxidant, anti-mutagenic, and anti-inflammatory activities. We previously showed that Jurkat cells, which express a high level of Fas, are very sensitive to treatment with avicins. Thus, we hypothesized that avicins may induce cell apoptosis by activation of the Fas pathway. By using a series of cell lines deficient in cell death receptors, we demonstrated that upon avicin D treatment, Fas translocates to the cholesterol- and sphingolipid-enriched membrane microdomains known as lipid rafts. In the lipid rafts, Fas interacts with Fas-associated death domain (FADD) and Caspase-8 to form death-inducing signaling complex (DISC) and thus mediates cell apoptosis. Interfering with lipid raft organization by using a cholesterol-depleting compound, methyl-β-cyclodextrin, not only prevents the clustering of Fas and its DISC complex but also reduces the sensitivity of the cells to avicin D. Avicin D activates Fas pathways independent of the association between extracellular Fas ligands and Fas receptors. A deficiency in Fas and its downstream signaling molecules leads to the resistance of the cells to avicin D treatment. Taken together, our results demonstrate that avicin D triggers the redistribution of Fas in the membrane lipid rafts, where Fas activates receptor-mediated cell death
Mass dependence of light nucleus production in ultrarelativistic heavy ion collisions
Light nuclei can be produced in the central reaction zone via coalescence in
relativistic heavy ion collisions. E864 at BNL has measured the production of
ten light nuclei with nuclear number of A=1 to A=7 at rapidity and
. Data were taken with a Au beam of momentum of 11.5 A
on a Pb or Pt target with different experimental settings. The
invariant yields show a striking exponential dependence on nuclear number with
a penalty factor of about 50 per additional nucleon. Detailed analysis reveals
that the production may depend on the spin factor of the nucleus and the
nuclear binding energy as well.Comment: (6 pages, 3 figures), some changes on text, references and figures'
lettering. To be published in PRL (13Dec1999
Measurements of Light Nuclei Production in 11.5 A GeV/c Au+Pb Heavy-Ion Collisions
We report on measurements by the E864 experiment at the BNL-AGS of the yields
of light nuclei in collisions of Au(197) with beam momentum of 11.5 A GeV/c on
targets of Pb(208) and Pt(197). The yields are reported for nuclei with baryon
number A=1 up to A=7, and typically cover a rapidity range from y(cm) to
y(cm)+1 and a transverse momentum range of approximately 0.1 < p(T)/A < 0.5
GeV/c. We calculate coalescence scale factors B(A) from which we extract model
dependent source dimensions and collective flow velocities. We also examine the
dependences of the yields on baryon number, spin, and isospin of the produced
nuclei.Comment: 21 figures-to be published in Phys. Rev.
Avicin D: A Protein Reactive Plant Isoprenoid Dephosphorylates Stat 3 by Regulating Both Kinase and Phosphatase Activities
Avicins, a class of electrophilic triterpenoids with pro-apoptotic, anti-inflammatory and antioxidant properties, have been shown to induce redox-dependant post-translational modification of cysteine residues to regulate protein function. Based on (a) the cross-talk that occurs between redox and phosphorylation processes, and (b) the role of Stat3 in the process of apoptosis and carcinogenesis, we chose to study the effects of avicins on the processes of phosphorylation/dephosphorylation in Stat3. Avicins dephosphorylate Stat3 in a variety of human tumor cell lines, leading to a decrease in the transcriptional activity of Stat3. The expression of Stat3-regulated proteins such as c-myc, cyclin D1, Bcl2, survivin and VEGF were reduced in response to avicin treatment. Underlying avicin-induced dephosphorylation of Stat3 was dephosphorylation of JAKs, as well as activation of protein phosphatase-1. Downregulation of both Stat3 activity and expression of Stat 3-controlled pro-survival proteins, contributes to the induction of apoptosis in avicin treated tumor cells. Based on the role of Stat3 in inflammation and wounding, and the in vivo inhibition of VEGF by avicins in a mouse skin carcinogenesis model, it is likely that avicin-induced inhibition of Stat3 activity results in the suppression of the pro-inflammatory and pro-oxidant stromal environment of tumors. Activation of PP-1, which also acts as a cellular economizer, combined with the redox regulation by avicins, can aid in redirecting metabolism from growth promoting anabolic to energy sparing pathways
Tunable variation of optical properties of polymer capped gold nanoparticles
Optical properties of polymer capped gold nanoparticles of various sizes
(diameter 3-6 nm) have been studied. We present a new scheme to extract size
dependent variation of total dielectric function of gold nanoparticles from
measured UV-Vis absorption data. The new scheme can also be used, in principle,
for other related systems as well. We show how quantum effect, surface atomic
co - ordination and polymer - nanoparticle interface morphology leads to a
systematic variation in inter band part of the dielectric function of gold
nanoparticles, obtained from the analysis using our new scheme. Careful
analysis enables identification of the possible changes to the electronic band
structure in such nanoparticles.Comment: 13 pages,7 figures, 1 tabl
Antideuteron yield at the AGS and coalescence implications
We present Experiment 864's measurement of invariant antideuteron yields in
11.5A GeV/c Au + Pt collisions. The analysis includes 250 million triggers
representing 14 billion 10% central interactions sampled for events with high
mass candidates. We find (1/2 pi pt) d^(2)N/dydpt = 3.5 +/- 1.5 (stat.)
+0.9,-0.5 (sys.) x 10^(-8) GeV^(-2)c^(2) for 1.8=0.35 GeV/c
(y(cm)=1.6) and 3.7 +/- 2.7 (stat.) +1.4,-1.5 (sys.) x 10^(-8) GeV^(-2)c^(2)
for 1.4=0.26 GeV/c, and a coalescence parameter B2-bar of 4.1 +/-
2.9 (stat.) +2.3,-2.4 (sys.) x 10^(-3) GeV^(2)c^(-3). Implications for the
coalescence model and antimatter annihilation are discussed.Comment: 8 pages, 4 figures, Latex, submitted to Phys. Rev. Let
101 Dothideomycetes genomes: A test case for predicting lifestyles and emergence of pathogens.
Dothideomycetes is the largest class of kingdom Fungi and comprises an incredible diversity of lifestyles, many of which have evolved multiple times. Plant pathogens represent a major ecological niche of the class Dothideomycetes and they are known to infect most major food crops and feedstocks for biomass and biofuel production. Studying the ecology and evolution of Dothideomycetes has significant implications for our fundamental understanding of fungal evolution, their adaptation to stress and host specificity, and practical implications with regard to the effects of climate change and on the food, feed, and livestock elements of the agro-economy. In this study, we present the first large-scale, whole-genome comparison of 101 Dothideomycetes introducing 55 newly sequenced species. The availability of whole-genome data produced a high-confidence phylogeny leading to reclassification of 25 organisms, provided a clearer picture of the relationships among the various families, and indicated that pathogenicity evolved multiple times within this class. We also identified gene family expansions and contractions across the Dothideomycetes phylogeny linked to ecological niches providing insights into genome evolution and adaptation across this group. Using machine-learning methods we classified fungi into lifestyle classes with >95 % accuracy and identified a small number of gene families that positively correlated with these distinctions. This can become a valuable tool for genome-based prediction of species lifestyle, especially for rarely seen and poorly studied species
Antiproton Production in 11.5 A GeV/c Au+Pb Nucleus-Nucleus Collisions
We present the first results from the E864 collaboration on the production of
antiprotons in 10% central 11.5 A GeV/c Au+Pb nucleus collisions at the
Brookhaven AGS. We report invariant multiplicities for antiproton production in
the kinematic region 1.4<y<2.2 and 50<p_T<300 MeV/c, and compare our data with
a first collision scaling model and previously published results from the E878
collaboration. The differences between the E864 and E878 antiproton
measurements and the implications for antihyperon production are discussed.Comment: 4 pages, 4 figures; accepted for publication in Physical Review
Letter
Muon `Depth -- Intensity' Relation Measured by LVD Underground Experiment and Cosmic-Ray Muon Spectrum at Sea Level
We present the analysis of the muon events with all muon multiplicities
collected during 21804 hours of operation of the first LVD tower. The measured
angular distribution of muon intensity has been converted to the `depth --
vertical intensity' relation in the depth range from 3 to 12 km w.e.. The
analysis of this relation allowed to derive the power index, , of the
primary all-nucleon spectrum: . The `depth -- vertical
intensity' relation has been converted to standard rock and the comparison with
the data of other experiments has been done. We present also the derived
vertical muon spectrum at sea level.Comment: 7 pages, 3 figures, to be published on Phys. Rev.
Upper Limit on the Prompt Muon Flux Derived from the LVD Underground Experiment
We present the analysis of the muon events with all muon multiplicities
collected during 21804 hours of operation of the first LVD tower. The measured
depth-angular distribution of muon intensities has been used to obtain the
normalization factor, A, the power index, gamma, of the primary all-nucleon
spectrum and the ratio, R_c, of prompt muon flux to that of pi-mesons - the
main parameters which determine the spectrum of cosmic ray muons at the sea
level. The value of gamma = 2.77 +/- 0.05 (68% C.L.) and R_c < 2.0 x 10^-3 (95%
C.L.) have been obtained. The upper limit to the prompt muon flux favours the
models of charm production based on QGSM and the dual parton model.Comment: 10 pages, 4 figures, RevTex. To appear in Phys. Rev.
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