Influence of surface recrystallization on the low cycle fatigue behaviour of a single crystal superalloy

This paper investigated the effect of surface recrystallization (RX) on the low cycle fatigue (LCF) behaviour of a single crystal (SX) superalloy. LCF tests on both raw and recrystallized samples showed that fatigue life was significantly reduced by surface RX. Fractography indicated that fatigue cracks initiated from the casting defects in RX layer and multiple crack initiations were commonly observed. Moreover, RX grains exhibited predominantly transgranular cracking, in contrast to the intergranular fracture reported in literature. The fatigue crack propagation behaviour was discussed in light of fracture mechanics and crack growth life model. The fatigue cycles required to penetrate RX layer were estimated to be about one magnitude lower than that in forming an equivalent crack in SX specimens. It is suggested that the earlier crack initiation and promoted crack propagation in RX layer, as well as the trend of multiple initiations, are responsible for the fatigue degradation by RX.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110545/1/ffe12236.pd

Former of Turn Trajectory of Sliding Valve Shaft of Gas Line

Former of turn trajectory of sliding valve shaft of gas line, that allows to provide desired motion trajectory of sliding valve and its full closing, is considered in that paper. Imitation model of that former, research results, which allow to detect influence of gain factor and time constant of position controller on value of speed error, that has impact on delay of output coordinate from setting, and that results to delay of sliding valve motion process to setting position point, are shown

Gene expression profiling of mesenteric lymph nodes from sheep with natural scrapie

Background: Prion diseases are characterized by the accumulation of the pathogenic PrPSc protein, mainly in the brain and the lymphoreticular system. Although prions multiply/accumulate in the lymph nodes without any detectable pathology, transcriptional changes in this tissue may reflect biological processes that contribute to the molecular pathogenesis of prion diseases. Little is known about the molecular processes that occur in the lymphoreticular system in early and late stages of prion disease. We performed a microarray-based study to identify genes that are differentially expressed at different disease stages in the mesenteric lymph node of sheep naturally infected with scrapie. Oligo DNA microarrays were used to identify gene-expression profiles in the early/middle (preclinical) and late (clinical) stages of the disease. Results: In the clinical stage of the disease, we detected 105 genes that were differentially expressed (=2-fold change in expression). Of these, 43 were upregulated and 62 downregulated as compared with age-matched negative controls. Fewer genes (50) were differentially expressed in the preclinical stage of the disease. Gene Ontology enrichment analysis revealed that the differentially expressed genes were largely associated with the following terms: glycoprotein, extracellular region, disulfide bond, cell cycle and extracellular matrix. Moreover, some of the annotated genes could be grouped into 3 specific signaling pathways: focal adhesion, PPAR signaling and ECM-receptor interaction. We discuss the relationship between the observed gene expression profiles and PrPSc deposition and the potential involvement in the pathogenesis of scrapie of 7 specific differentially expressed genes whose expression levels were confirmed by real time-PCR. Conclusions: The present findings identify new genes that may be involved in the pathogenesis of natural scrapie infection in the lymphoreticular system, and confirm previous reports describing scrapie-induced alterations in the expression of genes involved in protein misfolding, angiogenesis and the oxidative stress response. Further studies will be necessary to determine the role of these genes in prion replication, dissemination and in the response of the organism to this disease

Submarine slope failures along the convergent continental margin of the Middle America Trench

We present the first comprehensive study of mass wasting processes in the continental slope of a convergent margin of a subduction zone where tectonic processes are dominated by subduction erosion. We have used multibeam bathymetry along ∼1300 km of the Middle America Trench of the Central America Subduction Zone and deep-towed side-scan sonar data. We found abundant evidence of large-scale slope failures that were mostly previously unmapped. The features are classified into a variety of slope failure types, creating an inventory of 147 slope failure structures. Their type distribution and abundance define a segmentation of the continental slope in six sectors. The segmentation in slope stability processes does not appear to be related to slope preconditioning due to changes in physical properties of sediment, presence/absence of gas hydrates, or apparent changes in the hydrogeological system. The segmentation appears to be better explained by changes in slope preconditioning due to variations in tectonic processes. The region is an optimal setting to study how tectonic processes related to variations in intensity of subduction erosion and changes in relief of the underthrusting plate affect mass wasting processes of the continental slope. The largest slope failures occur offshore Costa Rica. There, subducting ridges and seamounts produce failures with up to hundreds of meters high headwalls, with detachment planes that penetrate deep into the continental margin, in some cases reaching the plate boundary. Offshore northern Costa Rica a smooth oceanic seafloor underthrusts the least disturbed continental slope. Offshore Nicaragua, the ocean plate is ornamented with smaller seamounts and horst and graben topography of variable intensity. Here mass wasting structures are numerous and comparatively smaller, but when combined, they affect a large part of the margin segment. Farther north, offshore El Salvador and Guatemala the downgoing plate has no large seamounts but well-defined horst and graben topography. Off El Salvador slope failure is least developed and mainly occurs in the uppermost continental slope at canyon walls. Off Guatemala mass wasting is abundant and possibly related to normal faulting across the slope. Collapse in the wake of subducting ocean plate topography is a likely failure trigger of slumps. Rapid oversteepening above subducting relief may trigger translational slides in the middle Nicaraguan upper Costa Rican slope. Earthquake shaking may be a trigger, but we interpret that slope failure rate is lower than recurrence time of large earthquakes in the region. Generally, our analysis indicates that the importance of mass wasting processes in the evolution of margins dominated by subduction erosion and its role in sediment dynamics may have been previously underestimated

Immune Responses and Pathogenesis following Experimental SARS-CoV-2 Infection in Domestic Cats

Several reports demonstrated the susceptibility of domestic cats to SARS-CoV-2 infection. Here, we describe a thorough investigation of the immune responses in cats after experimental SARS-CoV-2 inoculation, along with the characterization of infection kinetics and pathological lesions. Specific pathogen-free domestic cats ( n = 12) were intranasally inoculated with SARS-CoV-2 and subsequently sacrificed on DPI (days post-inoculation) 2, 4, 7 and 14. None of the infected cats developed clinical signs. Only mild histopathologic lung changes associated with virus antigen expression were observed mainly on DPI 4 and 7. Viral RNA was present until DPI 7, predominantly in nasal and throat swabs. The infectious virus could be isolated from the nose, trachea and lungs until DPI 7. In the swab samples, no biologically relevant SARS-CoV-2 mutations were observed over time. From DPI 7 onwards, all cats developed a humoral immune response. The cellular immune responses were limited to DPI 7. Cats showed an increase in CD8+ cells, and the subsequent RNA sequence analysis of CD4+ and CD8+ subsets revealed a prominent upregulation of antiviral and inflammatory genes on DPI 2. In conclusion, infected domestic cats developed a strong antiviral response and cleared the virus within the first week after infection without overt clinical signs and relevant virus mutations

SARS-CoV-2 infection in cats and dogs in infected mink farms

Animals like mink, cats and dogs are susceptible to SARS-CoV-2 infection. In the Netherlands, 69 out of 127 mink farms were infected with SARS-CoV-2 between April and November 2020 and all mink on infected farms were culled after SARS-CoV-2 infection to prevent further spread of the virus. On some farms, (feral) cats and dogs were present. This study provides insight into the prevalence of SARS-CoV-2-positive cats and dogs in 10 infected mink farms and their possible role in transmission of the virus. Throat and rectal swabs of 101 cats (12 domestic and 89 feral cats) and 13 dogs of 10 farms were tested for SARS-CoV-2 using PCR. Serological assays were performed on serum samples from 62 adult cats and all 13 dogs. Whole Genome Sequencing was performed on one cat sample. Cat-to-mink transmission parameters were estimated using data from all 10 farms. This study shows evidence of SARS-CoV-2 infection in 12 feral cats and 2 dogs. Eleven cats (18%) and two dogs (15%) tested serologically positive. Three feral cats (3%) and one dog (8%) tested PCR-positive. The sequence generated from the cat throat swab clustered with mink sequences from the same farm. The calculated rate of mink-to-cat transmission showed that cats on average had a chance of 12% (95%CI 10%–18%) of becoming infected by mink, assuming no cat-to-cat transmission. As only feral cats were infected it is most likely that infections in cats were initiated by mink, not by humans. Whether both dogs were infected by mink or humans remains inconclusive. This study presents one of the first reports of interspecies transmission of SARS-CoV-2 that does not involve humans, namely mink-to-cat transmission, which should also be considered as a potential risk for spread of SARS-CoV-2