39 research outputs found
Intracellular Persisting Staphylococcus aureus Is the Major Pathogen in Recurrent Tonsillitis
BACKGROUND: The two major indications for tonsillectomy are recurrent tonsillitis (RT) and peritonsillar abscess (PTA). Unlike PTAs, which are primarily treated surgically, RT is often cured by tonsillectomy only after a series of failed drug therapy attempts. Although the bacteriological background of RT has been studied, the reason for the lack of success of conservative therapeutic approaches is not well understood. METHODS: In a prospective study, tonsil specimens from 130 RT patients and 124 PTA patients were examined for the presence of extra- and intracellular bacteria using antibiotic protection assays. Staphylococcus aureus isolates from RT patients were characterized by pulsed-field gel electrophoresis (PFGE), spa-typing and MSCRAMM-gene-PCR. Their ability for biofilm formation was tested and their cell invasiveness was confirmed by a flow cytometric invasion assay (FACS), fluorescent in situ hybridization (FISH) and immunohistochemistry. FINDINGS: S. aureus was the predominant species (57.7%) in RT patients, whereas Streptococcus pyogenes was most prevalent (20.2%) in PTA patients. Three different assays (FACS, FISH, antibiotic protection assay) showed that nearly all RT-associated S. aureus strains were located inside tonsillar cells. Correspondingly, the results of the MSCRAMM-gene-PCRs confirmed that 87% of these S. aureus isolates were invasive strains and not mere colonizers. Based upon PFGE analyses of genomic DNA and on spa-gene typing the vast majority of the S. aureus isolates belonged to different clonal lineages. CONCLUSIONS: Our results demonstrate that intracellular residing S. aureus is the most common cause of RT and indicate that S. aureus uses this location to survive the effects of antibiotics and the host immune response. A German translation of the Abstract is provided as supplementary material (Abstract S1)
Az optikai koherenciatomográfia alkalmazási lehetőségei a fül-orr-gégészetben = Optical coherence tomography in otolaryngology
Az optikai koherenciatomográfia a koherens fĂ©nynyalábok interferenciáján alapulĂł kĂ©palkotĂł eljárás. Mivel segĂtsĂ©gĂ©vel noninvazĂv mĂłdon, mikromĂ©teres nagyságrendben vizsgálhatĂłak az Ă©lĹ‘ szövetek, a medicina számos terĂĽletĂ©n alkalmazzák. A szerzĹ‘k cĂ©lja, hogy áttekintĂ©st adjanak az optikai koherenciatomográfia felhasználási lehetĹ‘sĂ©geirĹ‘l a fĂĽl-orr-gĂ©gĂ©szetben. Saját vizsgálataik Ă©s az irodalmi adatok alapján az optikai koherenciatomográfia alkalmas a humán gĂ©ge- Ă©s garatnyálkahártya gyulladásos, rákmegelĹ‘zĹ‘ Ă©s malignus állapotainak intraoperatĂv elkĂĽlönĂtĂ©sĂ©re Ă©s lehetĹ‘vĂ© teszi a daganatos szövetek cĂ©lzott biopsziáját. A fej-nyaki rĂ©giĂł nyálkahártyájának vizsgálata mellett bizonyos közĂ©pfĂĽlbetegsĂ©gek, mint pĂ©ldául a stapesfixatio Ă©s cholesteatoma diagnosztikájában is szerepet kaphat az optikai koherenciatomográfia. Orv. Hetil., 2011, 152, 1125–1132.
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Optical coherence tomography is an imaging technique based on coherence interferometry. It is used in many medical fields due to its non-invasive imaging capabilities with micrometer resolution. The aim of the authors was to review the applicability of the optical coherence tomography in otolaryngology. Literature data and their own studies show that optical coherence tomography is a reliable method for identifying and targeted biopsy of inflammated, pre-malignant or cancer tissue in human laryngeal and pharyngeal mucosa. Another emerging field for optical coherence tomography is diagnosis of middle ear diseases, especially stapes ankylosis and cholesteatoma. Orv. Hetil., 2011, 152, 1125–1132.<inline-graphic xlink:href="fig5a.jpg"/
Effect of Tissue Heterogeneity on the Transmembrane Potential of Type-1 Spiral Ganglion Neurons: A Simulation Study
Optotensometry – Developement of an optic method for measuring tubal function by tympanic membrane movement in a middle ear model
Automatic Detection of the Electrical Elicited Stapedius Reflex by Evaluation of Its Electromyographic Signals
Anesthesia with Propofol versus Sevoflurane: Does the Longer Neuromuscular Block under Sevoflurane Anesthesia Reduce Laryngeal Injuries?
Anesthesia can be maintained with propofol or sevoflurane. Volatile anesthetics increase neuromuscular block of muscle relaxants. We tested the hypothesis, that sevoflurane would cause less vocal cord injuries than an intravenous anesthesia with propofol. In this prospective trial, 65 patients were randomized in 2 groups: SEVO group, anesthesia with sevoflurane, and TIVA group, total intravenous anesthesia with propofol. Intubating and extubating conditions were evaluated. Vocal cord injuries were examined by stroboscopy before and 24 and 72 h after surgery; hoarseness and sore throat were assessed up to 72 h after surgery. Hoarseness and sore throat were comparable between both groups (not significant). Similar findings were observed for vocal cord injuries: 9 (SEVO) versus 5 (TIVA) patients; ; the overall incidence was 24%. Type of vocal cord injuries: 9 erythema and 5 edema of the vocal folds. Neuromuscular block was significantly longer in the SEVO group compared with the TIVA group: 71 (range: 38–148) min versus 52 (range: 21–74) min; . Five patients (TIVA group) versus 11 patients (SEVO group) needed neostigmine to achieve a TOF ratio of 1.0 . Under anesthesia with propofol laryngeal injuries were not increased; the risk for residual curarization, however, was lower compared with sevoflurane