Appendix B. Time-averaged population matrices and corresponding population growth rates λ for seven Primula elatior populations located in forests with different histories of previous land use.

Time-averaged population matrices and corresponding population growth rates λ for seven Primula elatior populations located in forests with different histories of previous land use

Appendix A. Soil chemical properties of the investigated forests.

Soil chemical properties of the investigated forests

GeneMapper data of 560 individuals of Saxifraga granulata collected along two rivers in Belgium

Raw GeneMapper data with four extra columns with information about the samples (i.e. Sort, River, Population, Individual). Without the first four columns data can be easily read via the r function read.GeneMapper() from the r-package 'polysat'

GenAlEx input file

This file contains the raw microsatellite (SSR) dataset for all sampled Anthyllis vulneraria individuals (N=896) in GenAlEx format. This file can be converted to other formats using the program GenAlEx

Study area in Belgium and locations of the 28 sampling sites along the rivers Dijle and Demer.

<p>The arrows indicate the direction of the water flow. The figure was constructed based on maps of the NGI (Nationaal Geographisch Instituut) using QGIS 2.6.0 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130463#pone.0130463.ref065" target="_blank">65</a>].</p

The relationship between pairwise genetic distances (<i>G”</i>_ST) and log transformed geographic distances (ln(m)) for (A) 15 populations of <i>S</i>. <i>granulata</i> along the Dijle River.

<p>Black triangles denote distances from the highly differentiated population ‘Heverlee’ to the other populations and grey circles represent distances between the remaining 14 populations. The result of the mantel test that included population ‘Heverlee’ was not significant, however, we did find a significant isolation-by-distance effect between the other 14 populations (Mantel’s r = 0.327; <i>P</i> = 0.048). (B) 13 Populations along the Demer River with a significant isolation-by-distance effect (Mantel’s r = 0.231; <i>P</i> = 0.036).</p

Spagedi input files

This file contains all eight input files used for the Spagedi analyses on the georeferenced Anthyllis vulneraria populations. This file contains both microsatellite (SSR) data and geographical location data

K-means clustering analysis of populations (A) along the Dijle River, showing populations divided into 2–6 clusters.

<p>(B) Clusters along the Demer River.</p

Sampling locations

This file contains the GPS sampling locations of all geographically measured Anthyllis vulneraria plants (N=730). Coordinates are given in both Lambert72 and in WGS84. Plant ID's correspond to those in the 'GenAlEx input file'

Characteristics of the studied <i>Saxifraga granulata</i> populations along the river systems Dijle and Demer.

<p>Populations are ordered according to their position along the river, the upper most population in the table occurs most upstream.</p><p><i>H</i>_S is the heterozygosity within populations, <i>H</i>_T the total heterozygosity for both river systems.</p><p>* Sampling locations situated in grasslands managed by ‘Natuurpunt’.</p><p>Characteristics of the studied <i>Saxifraga granulata</i> populations along the river systems Dijle and Demer.</p