Turbulent mixing at a shear-free density interface

The interaction of a sharp density interface with oscillating-grid-induced shear-free turbulence was experimentally investigated. A linear photodiode array was used in conjunction with laser-induced fluorescence to measure the concentration of dye that was initially only in the less dense layer. A laser-Doppler velocimeter was used to measure the vertical velocity in and above the density interface at a point where the dye concentration was also measured. Potential refractive-index-fluctuation problems were avoided using solutes that provided a homogeneous optical environment across the density interface. Internal wave spectra, amplitudes and velocities, as well as the vertical mass flux were measured. The results indicate that mixing occurs in intermittent bursts and that the gradient (local) Richardson number remains constant for a certain range of the overall Richardson number R_j, defined in terms of an integral lengthscale, buoyancy jump and turbulence intensity. The spectra of the internal waves decay as f^(−3) at frequencies below the maximum Brunt-Väisälä frequency. These findings give support to a model for oceanic mixing proposed by Phillips (1977) in which the internal waves are limited in their spectral density by sporadic local instabilities and breakdown to turbulence. The results also indicate that, for a certain R_j range, the thickness of the interfacial layer (normalized by the integral lengthscale of the turbulence) is a decreasing function of R_j. At sufficiently high R_j the interfacial thickness becomes limited by diffusive effects. Finally, we discuss a simple model for entrainment at a density interface in the presence of shear-free turbulence

Turbulence structure near a sharp density interface

The effects of a sharp density interface and a rigid flat plate on oscillating-grid induced shear-free turbulence were investigated experimentally. A two-component laser-Doppler velocimeter was used to measure turbulence intensities in and above the density interface (with matched refractive indices) and near the rigid flat plate. Energy spectra, velocity correlations, and kinetic energy fluxes were also measured. Amplification of the horizontal turbulent velocity, coupled with a sharp reduction in the vertical turbulent velocity, was observed near both the density interface and the flat plate. These findings are in agreement with some previous results pertaining to shear-free turbulence near rigid walls (Hunt & Graham 1978) and near density interfaces (Long 1978). The results imply that, near the density interface, the turbulent kinetic energy in the vertical velocity component is only a small fraction of the total turbulent kinetic energy and indicate that the effects of the anisotropy created by the density interface or the flat plate are confined to the large turbulence scales

Vascular endothelial growth factor-A and Poly(A) binding protein-interacting protein 2 expression in human head and neck carcinomas: correlation and prognostic significance

Vascular endothelial growth factor-A (VEGF-A) has been demonstrated to play an important role in tumour angiogenesis and to influence prognosis in many cancers. However its prognostic value in head and neck squamous cell carcinomas (HNSCCs) remains controversial. Therefore, we investigated the clinical relevance of VEGF-A expression in HNSCCs and analysed whether its expression was associated with PAIP2 protein levels, a VEGF-A mRNA-binding partner that strongly regulates VEGF-A expression in tissue culture. We determined the correlation of VEGF-A and PAIP2 protein levels, quantitatively evaluated in tumour tissue homogenates from 54 patients with HNSCC, to clinicopathological parameters. We showed that VEGF-A expression in HNSCC is correlated to the stage of tumour differentiation (P=0.050) and is an independent prognostic factor for progression-free survival (P=0.001) and overall survival (P=0.0004). In a pharynx carcinoma cell line, we demonstrated by RNA interference that VEGF-A expression is closely controlled by PAIP2. Moreover, in human HNSCCs, VEGF-A expression is significantly correlated to PAIP2 protein levels (P=0.0018). Nevertheless, PAIP2 expression is associated with neither clinicopathological factors nor patient's survival. Our data suggest that, in contrast to PAIP2 protein levels, which are unrelated to tumour prognosis, VEGF-A expression could serve as a prognostic marker in head and neck cancer and may be helpful for targeted therapies

Thermoclinic Assessment Of A Preliminary Circulation Model For Lake George In The Jefferson Project

The Jefferson Project is a collaboration between the Rensselaer Polytechnic Institute, IBM, and the FUND for Lake George aimed at understanding and managing complex factors (road salt, storm water runoff, invasive species) threatening Lake George, New York. Lake George is located about 80 km north of Albany in upstate New York and is known internationally for its water clarity. Understanding the hydrodynamics of the lake is fundamental for creation and maintenance of a research and monitoring program for the early detection of and response to adverse environmental and biological change. In this work a 3D circulation model of the lake is developed to better understand the hydro-environmental conditions of the lake; forcing is by a combination of local public survey data for the water budget and atmospheric data from the NWS (NOAA National Weather Service). The model is validated by a combination of water chemistry data collected by Darrin Fresh Water Institute (DFWI) over the last three decades, and known empirical relationships of the lake\u27s structural profile. Numerical simulations run over several years to capture the seasonal progression of thermocline depth throughout the lake, the south to north salt and surface thermal gradients and the timing of the spring and fall overturn events. Validation is by comparison with physical and chemical measurements collected over the last three decades. The study presents a novel combination of observational data, numerical modelling and empirical relationships to better understand and predict the lake circulation, and consequently the natural ecosystem

Correlation between Group B Streptococcal Genotypes, Their Antimicrobial Resistance Profiles, and Virulence Genes among Pregnant Women in Lebanon

The antimicrobial susceptibility profiles of 76 Streptococcus agalactiae (Group B Streptococci [GBS]) isolates from vaginal specimens of pregnant women near term were correlated to their genotypes generated by Random Amplified Polymorphic DNA analysis and their virulence factors encoding genes cylE, lmb, scpB, rib, and bca by PCR. Based on the distribution of the susceptibility patterns, six profiles were generated. RAPD analysis detected 7 clusters of genotypes. The cylE gene was present in 99% of the isolates, the lmb in 96%, scpB in 94.7%, rib in 33%, and bca in 56.5% of isolates. The isolates demonstrated a significant correlation between antimicrobial resistance and genotype clusters denoting the distribution of particular clones with different antimicrobial resistance profiles, entailing the practice of caution in therapeutic options. All virulence factors encoding genes were detected in all seven genotypic clusters with rib and bca not coexisting in the same genome

Hepatitis B Virus Genotyping Among Chronic Hepatitis B Individuals With Resistance to Lamivudine in Shahrekord, Iran

Background: Hepatitis B infection, caused by hepatitis B Virus (HBV), is one of the major global public health problems. Hepatitis B Virus genotypes appear to show varying geographic distribution with possible pathogenic and therapeutic differences. Knowledge of HBV genotypes is very important for clinical treatment. Lamivudine is a nucleoside analogue that is clinically used to treat chronic hepatitis B infection. However, the main problem with the application of lamivudine is the development of viral resistance to the treatment with this anti viral drug. Besides, it has been suggested that lamivudine-resistant HBV may be genotype dependent. However, HBV genotype distribution and the biological relevance in this region are poorly understood. Objectives: The current study aimed to determine hepatitis B genotypes and their correlation with lamivudine-resistant HBV frequency among patients with chronic hepatitis B from Shahrekord, Iran. Methods and Materials: Hepatitis B virus DNA was detected by conventional PCR in some of the serum samples obtained from HBsAg-positive Chronic Hepatitis B (CHB) patients who were referred to Health Centers of Shahrekord for routine monitoring of the disease. Subsequently, using real-time PCR, the DNA samples were used for genotyping and analysis of resistance to lamivudine. Results: The DNA was detected in 23 out of 116 (19.82%) of the studied samples. Genotypes D and C were found in 17 out of 23 (73.9%), and in 6 out of 23 (26.1%) of the samples, respectively. To the authors' best knowledge, the current study is the first report on isolation of Genotype C from Iran. Two out of 17 (11.76%), and 6 out of 6 (100%) of genotypes D and C were resistant to lamivudine, respectively. Resistance to this drug was significantly different between genotypes C and D (P < 0.001). Conclusions: In addition to genotype D, other lamivudine resistant hepatitis B genotypes might be distributed in Iran

Pyrocarbon versus cobalt-chromium in the context of spherical interposition implants: an in vitro study on cultured chondrocytes

In the context of shoulder surgical replacement, a new generation of spherical interposition implants has been developed, with the implant being a mobile spacer rubbing against the glenoid cartilage and humeral bone cavity. The aim of the present study was to compare pyrocarbon (PyC) versus cobalt-chromium (CoCr) implants, regarding preservation and regeneration of the surrounding tissues. The effect of the biomaterials on chondrocytes was analysed in vitro. Murine primary chondrocytes were grown on discs made of PyC or CoCr using two culture media to mimic either cartilage-like or bone-like conditions (CLC or BLC). Chondrocytes did grow on PyC and CoCr without alteration in cell viability or manifestation of cytotoxicity. The tissue-like cell membranes grown under BLC were examined for the chondrocyte’s ability to mineralise (by alizarin red matrix staining, calcium deposit and alkaline phosphatase activity) and for their mechanical properties (by rheological tests). For the chondrocytes grown under CLC and BLC, extracellular matrix components were analysed by histological staining and immunolabelling. Under CLC, PyC promoted type II collagen expression in chondrocytes, suggesting that they may generate a more cartilage-like matrix than samples grown on both CoCr and plastic control. In BLC, the tissue-like cell membranes grown on PyC were more mineralised and homogenous. The mechanical results corroborated the biological data, since the elastic modulus of the tissue-like cell membranes developed on the PyC surface was higher, indicating more stiffness. Overall, the results suggested that PyC might be a suitable biomaterial for spherical interposition implants

Cerebellar Ataxia With Anti-DNER Antibodies: Outcomes and Immunologic Features

BACKGROUND AND OBJECTIVES: There is no report on the long-term outcomes of ataxia with antibodies against Delta and Notch-like epidermal growth factor-related (DNER). We aimed to describe the clinical-immunologic features and long-term outcomes of patients with anti-DNER antibodies. METHODS: Patients tested positive for anti-DNER antibodies between 2000 and 2020 were identified retrospectively. In those with available samples, immunoglobulin G (IgG) subclass analysis, longitudinal cerebellum volumetry, human leukocyte antigen isotyping, and CSF proteomic analysis were performed. Rodent brain membrane fractionation and organotypic cerebellar slices were used to study DNER cell-surface expression and human IgG binding to the Purkinje cell surface. RESULTS: Twenty-eight patients were included (median age, 52 years, range 19-81): 23 of 28 (82.1%) were male and 23 of 28 (82.1%) had a hematologic malignancy. Most patients (27/28, 96.4%) had cerebellar ataxia; 16 of 28 (57.1%) had noncerebellar symptoms (cognitive impairment, neuropathy, and/or seizures), and 27 of 28 (96.4%) became moderately to severely disabled. Half of the patients (50%) improved, and 32.1% (9/28) had no or slight disability at the last visit (median, 26 months; range, 3-238). Good outcome significantly associated with younger age, milder clinical presentations, and less decrease of cerebellar gray matter volumes at follow-up. No human leukocyte antigen association was identified. Inflammation-related proteins were overexpressed in the patients' CSF. In the rodent brain, DNER was enriched in plasma membrane fractions. Patients' anti-DNER antibodies were predominantly IgG1/3 and bound live Purkinje cells in vitro. DISCUSSION: DNER ataxia is a treatable condition in which nearly a third of patients have a favorable outcome. DNER antibodies bind to the surface of Purkinje cells and are therefore potentially pathogenic, supporting the use of B-cell-targeting treatments