Changes in neuronal activity across the mouse ventromedial nucleus of the hypothalamus in response to low glucose: evaluation using an extracellular multi‐electrode array approach

The hypothalamic ventromedial nucleus (VMN) is involved in maintaining systemic glucose homeostasis. Neurophysiological studies in rodent brain slices have identified populations of VMN glucose‐sensing neurones: glucose‐excited (GE) neurones, cells which increased their firing rate in response to increases in glucose concentration, and glucose‐inhibited (GI) neurones, which show a reduced firing frequency in response to increasing glucose concentrations. To date, most slice electrophysiological studies characterising VMN glucose‐sensing neurones in rodents have utilised the patch clamp technique. Multi‐electrode arrays (MEAs) are a state‐of‐the‐art electrophysiological tool enabling the electrical activity of many cells to be recorded across multiple electrode sites (channels) simultaneously. We used a perforated MEA (pMEA) system to evaluate electrical activity changes across the dorsal‐ventral extent of the mouse VMN region in response to alterations in glucose concentration. Because intrinsic (ie, direct postsynaptic sensing) and extrinsic (ie, presynaptically modulated) glucosensation were not discriminated, we use the terminology ‘GE/presynaptically excited by an increase (PER)’ and ‘GI/presynaptically excited by a decrease (PED)’ in the present study to describe responsiveness to changes in extracellular glucose across the mouse VMN. We observed that 15%‐60% of channels were GE/PER, whereas 2%‐7% were GI/PED channels. Within the dorsomedial portion of the VMN (DM‐VMN), significantly more channels were GE/PER compared to the ventrolateral portion of the VMN (VL‐VMN). However, GE/PER channels within the VL‐VMN showed a significantly higher basal firing rate in 2.5 mmol l‐1 glucose than DM‐VMN GE/PER channels. No significant difference in the distribution of GI/PED channels was observed between the VMN subregions. The results of the present study demonstrate the utility of the pMEA approach for evaluating glucose responsivity across the mouse VMN. pMEA studies could be used to refine our understanding of other neuroendocrine systems by examining population level changes in electrical activity across brain nuclei, thus providing key functional neuroanatomical information to complement and inform the design of single‐cell neurophysiological studies

Spectral and stratigraphic mapping of hydrated sulfate and phyllosilicate-bearing deposits in northern Sinus Meridiani, Mars

We present detailed stratigraphic and spectral analyses that focus on a region in northern Sinus Meridiani located between 1°N to 5°N latitude and 3°W to 1°E longitude. Several stratigraphically distinct units are defined and mapped using morphologic expression, spectral properties, and superposition relationships. Previously unreported exposures of hydrated sulfates and Fe/Mg smectites are identified using MRO CRISM and MEX OMEGA near‐infrared (1.0 to 2.5 µm) spectral reflectance observations. Layered deposits with monohydrated and polyhydrated sulfate spectral signatures that occur in association with a northeast‐southwest trending valley are reexamined using highresolution CRISM, HiRISE, and CTX images. Layers that are spectrally dominated by monohydrated and polyhydrated sulfates are intercalated. The observed compositional layering implies that multiple wetting events, brine recharge, or fluctuations in evaporation rate occurred. We infer that these hydrated sulfate‐bearing layers were unconformably deposited following the extensive erosion of preexisting layered sedimentary rocks and may postdate the formation of the sulfate‐ and hematite‐bearing unit analyzed by the MER Opportunity rover. Therefore, at least two episodes of deposition separated by an unconformity occurred. Fe/Mg phyllosilicates are detected in units that predate the sulfateand hematite‐bearing unit. The presence of Fe/Mg smectite in older units indicates that the relatively low pH formation conditions inferred for the younger sulfate‐ and hematitebearing unit are not representative of the aqueous geochemical environment that prevailed during the formation and alteration of earlier materials. Sedimentary deposits indicative of a complex aqueous history that evolved over time are preserved in Sinus Meridiani, Mars

Spin Susceptibility and Gap Structure of the Fractional-Statistics Gas

This paper establishes and tests procedures which can determine the electron energy gap of the high-temperature superconductors using the $t\!-\!J$ model with spinon and holon quasiparticles obeying fractional statistics. A simpler problem with similar physics, the spin susceptibility spectrum of the spin 1/2 fractional-statistics gas, is studied. Interactions with the density oscillations of the system substantially decrease the spin gap to a value of $(0.2 \pm 0.2)$ $\hbar \omega_c$, much less than the mean-field value of $\hbar\omega_c$. The lower few Landau levels remain visible, though broadened and shifted, in the spin susceptibility. As a check of the methods, the single-particle Green's function of the non-interacting Bose gas viewed in the fermionic representation, as computed by the same approximation scheme, agrees well with the exact results. The same mechanism would reduce the gap of the $t\!-\!J$ model without eliminating it.Comment: 35 pages, written in REVTeX, 16 figures available upon request from [email protected]

Spin Transition in Strongly Correlated Bilayer Two Dimensional Electron Systems

Using a combination of heat pulse and nuclear magnetic resonance techniques we demonstrate that the phase boundary separating the interlayer phase coherent quantum Hall effect at $\nu_T = 1$ in bilayer electron gases from the weakly coupled compressible phase depends upon the spin polarization of the nuclei in the host semiconductor crystal. Our results strongly suggest that, contrary to the usual assumption, the transition is attended by a change in the electronic spin polarization.Comment: 4 pages, 3 postscript figur

Mutations in human dynamin block an intermediate stage in coated vesicle formation

The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to block endocytosis at a stage after the initiation of coat assembly and preceding the sequestration of ligands into deeply invaginated coated pits. Membrane transport from the ER to the Golgi complex was unaffected indicating that dynamin mutants specifically block early events in endocytosis. These results demonstrate that mutations in the GTP-binding domain of dynamin block Tfn-endocytosis in mammalian cells and suggest that a functional dynamin GTPase is required for receptor-mediated endocytosis via clathrin-coated pits

Onset of Interlayer Phase Coherence in a Bilayer Two-Dimensional Electron System: Effect of Layer Density Imbalance

Tunneling and Coulomb drag are sensitive probes of spontaneous interlayer phase coherence in bilayer two-dimensional electron systems at total Landau level filling factor $\nu_T = 1$. We find that the phase boundary between the interlayer phase coherent state and the weakly-coupled compressible phase moves to larger layer separations as the electron density distribution in the bilayer is imbalanced. The critical layer separation increases quadratically with layer density difference.Comment: 4 pages, 3 figure

Singular value decomposition applied to compact binary coalescence gravitational-wave signals

We investigate the application of the singular value decomposition to compact-binary, gravitational-wave data-analysis. We find that the truncated singular value decomposition reduces the number of filters required to analyze a given region of parameter space of compact binary coalescence waveforms by an order of magnitude with high reconstruction accuracy. We also compute an analytic expression for the expected signal-loss due to the singular value decomposition truncation.Comment: 4 figures, 6 page