The spindle of oocytes observed by polarized light microscope can predict embryo quality

Background: The aim is to evaluate spindle position of metaphase II oocyte and the development of embryos originated from oocytes with spindle and without spindle.Methods: Cross-sectional analysis Research: 250 MII oocytes were analyzed with polarized microscope in Military Institute of Clinical Embryology and Histology, Vietnam Military Medical University.Results: Spindles were detected in 170 (77.98%) of 218 metaphase II oocytes, 115 spindles (67.65%) of MII oocytes is beneath or adjacent to the first polar body, 55 oocytes had the spindle located between 300 and 1800 away from the first polar body. Fertilization rate and the rate of good quality embryos in oocytes with a visible spindle (77.98% and 61.02%) were higher than those in oocytes without a visible spindle (22.02% and 36.84%), the difference was statistically significant with p <0.001 and p <0.05.Conclusions: The spindle position of metaphase II oocytes is not always beneath or adjacent to the first polar body. Fertilization rate and the rate of good quality embryos in oocytes with a visible spindle were higher than those in oocytes without a visible spindle

PHÁT TRIỂN DU LỊCH CHĂM SÓC SỨC KHỎE TẠI TỈNH THỪA THIÊN HUẾ: GÓC NHÌN TỪ KHÁCH DU LỊCH

Wellness tourism has been around for a long time, and in the context of the pandemic, it has emerged as a new global tourism trend. Vietnam in general, and Thua Thien Hue in particular, have a lot of promise, but there aren't any specific products or services to help boost wellness tourism. Based on liturature review, the study focuses on estimating the necessity of wellness services/activities that should be available in Hue based on six major groups of: (1) physical; (2) mind and body; (3) spiritual and self-connected; (4) personal growth; (5) nature-oriented; and (6) social activities. Using a questionnaire survey method with 200 tourists who are knowledgeable about wellness, a number of activities such as visiting natural landscapes, cycling, walking, spending time experiencing with family/friends, reading books, spending time for yourself, bathing in hot springs... were rated as highly necessary. Simultaneously, the results demonstrate that different groups of tourists have varied perspectives based on demographic criterion. Some orientations and proposals for developing wellness tourism products in Thua Thien Hue province are offered through 6 main solutions on primary groups of activities.Du lịch chăm sóc sức khỏe đã xuất hiện từ lâu và trong bối cảnh dịch bệnh đã trở thành một xu hướng mới của du lịch thế giới. Việt Nam nói chung và Thừa Thiên Huế nói riêng có rất nhiều tiềm năng nhưng chưa có sản phẩm dịch vụ cụ thể để phát triển du lịch chăm sóc sức khỏe. Dựa trên tổng quan lý thuyết, nghiên cứu tập trung xác định mức độ cần thiết của các dịch vụ/ hoạt động CSSK nên có ở Huế trên cơ sở 6 nhóm hoạt động chính về: (1) thể chất; (2) tâm trí và cơ thể; (3) tâm linh và kết nối bản thân; (4) cảm xúc cá nhân; (5) thiên nhiên và (6) xã hội. Sử dụng phương pháp điều tra bằng bảng hỏi với 200 khách du lịch có hiểu biết về chăm sóc sức khỏe, một số hoạt động được đánh giá ở mức độ cần thiết cao như: tham quan các cảnh quan thiên nhiên, đạp xe, đi bộ, dành thời gian trải nghiệm cùng gia đình/bạn bè, đọc sách, dành thời gian cho bản thân, tắm suối khoáng nóng… Đồng thời, kết quả cũng cho thấy sự khác biệt trong ý kiến đánh giá của các nhóm du khách theo một số đặc điểm nhân khẩu học. Từ đó, bài viết đề xuất 6 nhóm giải pháp về phát triển sản phẩm du lịch chăm sóc sức khỏe tại tỉnh Thừa Thiên Huế

Study change of the performance of airfoil of small wind turbine under low wind speed by CFD simulation

Renewable energy has received strong attention and investment to replace fossil energy sources and reduce greenhouse gas emissions. Quite good and good wind speed areas have been invested in building large-capacity wind farms for many years. The low wind speed region occupies a very large on the world, which has been interested in the exploitation of wind energy in recent years. In this study, the original airfoil of S1010 operated at low wind speed was redesigned to increase the aerodynamic efficiency of the airfoil by using XFLR5 software. After, the new VAST-EPU-S1010 airfoil model was adjusted to the maximum thickness and the maximum thickness position. It was simulated in low wind speed conditions of 4-6 m/s by CFD simulation. The lift coefficient, drag coefficient and $C_{L}$/$C_{D}$ coefficient ratio were evaluated under the effect of the angle of attack and the maximum thickness by using the $k-\epsilon$ model. Simulation results show that the VAST-EPU-S1010 airfoil achieved the greatest aerodynamic efficiency at the angle of attack of 3\,^{\circ}, the maximum thickness of 8\% and the maximum thickness position of 20.32\%. The maximum value of $C_{L}$/$C_{D}$ of the new airfoil at 6 m/s is higher than at the 4 m/s by about 6.25\%.Comment: 19 pages, 21 figure

Radicular cyst in a primary molar following pulp therapy with gutta percha : a case report and literature review

A radicular cyst (RC) in deciduous dentition is relatively rare. This clinical report presents a case of RC that condition derived from a primary molar undergone an endodontic treatment with gutta-percha approximately one year ago. In addition, we also considered whether intracanal medicaments and gutta-percha filling material related to the formation and development of the cyst or not

The Status of Educational Sciences In Vietnam: A Bibliometric Analysis From Clarivate Web Of Science Database Between 1991 And 2018

Since 2013, Vietnam has implemented a plan to reform the whole education sector. However, there is little understanding on the status of educational research in Vietnam, which may lay the foundation for such plan. Thus, this research aims to analyze the whole picture of educational research from Vietnam, as seen from the Clarivate Web of Science (WOS) database: 215 publications were recorded, ranging from 1991 to 2018. These 215 publications were further analyzed from five perspectives: 1) number of publications by year; 2) research fields and levels of education; 3) top institutions with the highest number of publications; 4) international collaboration; and 5) quality. Some of the most notable results are: 1) the educational sciences in Vietnam have been still under-developed until recently; 2) among different research topics research among educational sciences, some (e.g., Vocational Education and Training or Early Childhood Education) seemed to be overlooked whereas others (e.g., Higher Education and Teaching and Learning) seemed to receive more attention from educational scholars; 3) all the most major education – specialized universities did not appear among the top five institutions with highest number of publications; 4) Australia, Thailand, the USA, New Zealand and China were the countries with the highest number of co-publications with Vietnamese researchers; and 5) The majority of publications belonged to low-ranked journals. Implications would be withdrawn for Vietnamese policymakers, education leaders, educational researchers and teachers in order to adjust their policies and/or action plans; thus, enhancing the performance and impacts of educational research in the future

Prospective evaluation of GeneXpert for the diagnosis of HIV- negative pediatric TB cases

Background The GeneXpertMTB/RIF (Xpert) assay is now recommended by WHO for diagnosis of tuberculosis (TB) in children but evaluation data is limited. Methods One hundred and fifty consecutive HIV negative children (<15 years of age) presenting with suspected TB were enrolled at a TB referral hospital in Ho Chi Minh City, Vietnam. 302 samples including sputum (n = 79), gastric fluid (n = 215), CSF (n = 3), pleural fluid (n = 4) and cervical lymphadenopathic pus (n = 1) were tested by smear, automated liquid culture (Bactec MGIT) and Xpert. Patients were classified retrospectively using the standardised case definition into confirmed, probable, possible, TB unlikely or not TB categories. Test accuracy was evaluated against 2 gold standards: [1] clinical (confirmed, probable and possible TB) and [2] ‘confirmed TB’ alone. Results The median age of participants was 18 months [IQR 5–170]. When test results were aggregated by patient, the sensitivity of smear, Xpert and MGIT against clinical diagnosis as the gold standard were 9.2% (n = 12/131) [95%CI 4.2; 14.1], 20.6% (n = 27/131) [95%CI 13.7; 27.5] and 29.0% (n = 38/131) [21.2;36.8], respectively. Specificity 100% (n = 19/19), 94.7% (n = 18/19), 94.7% (n = 18/19), respectively. Xpert was more sensitive than smear (P = <0.001) and less sensitive than MGIT (P = 0.002). Conclusions The systematic use of Xpert will increase early TB case confirmation in children and represents a major advance but sensitivity of all tests remains unacceptably low. Improved rapid diagnostic tests and algorithm approaches for pediatric TB are still an urgent research priority

Study on the antioxidant capacity of Hibiscus rosa-sinensis decoction in vivo in Mus musculus var. albino

This study proved that Hibiscus rosa-sinensis has the antioxidant activity and the ability to recover liver functions when it was coordinated with cyclophosphamide in mice mode

Synthesis and biological evaluation of shikimic acid derivatives

From shikimic acid, new series of oseltamivir analogues containing either ether sulfur or ether oxygen at C-3 position were prepared and evaluated for their biological activities. This is the first report on synthesis of oseltamivir analogues with ether sulfur at C-3. Except for compounds 4d, 4f, 7c and 7d, the remaining compounds were found to be active against MCF-7, LU-1 and KB cell lines. As oseltamivir had no cytotoxicity against different cell lines such as MCF-7, LU-1, KB, MDCK, MRC-5, VERO, MK and 293, the modification of alkyl groups at C-3 of the oseltamivir ring framework could significantly increase the cytotoxicity for this class of compounds. Loss of neuraminidase inhibition activity of these synthetic oseltamivir analogues suggested that the alkyl ether groups at C-3 should be critical for their anti-neuraminidase activity

Antibiotic use and prescription and its effects on Enterobacteriaceae in the gut in children with mild respiratory infections in Ho Chi Minh City, Vietnam. A prospective observational outpatient study.

BACKGROUND AND OBJECTIVES: Treatment guidelines do not recommend antibiotic use for acute respiratory infections (ARI), except for streptococcal pharyngitis/tonsillitis and pneumonia. However, antibiotics are prescribed frequently for children with ARI, often in absence of evidence for bacterial infection. The objectives of this study were 1) to assess the appropriateness of antibiotic prescriptions for mild ARI in paediatric outpatients in relation to available guidelines and detected pathogens, 2) to assess antibiotic use on presentation using questionnaires and detection in urine 3) to assess the carriage rates and proportions of resistant intestinal Enterobacteriaceae before, during and after consultation. MATERIALS AND METHODS: Patients were prospectively enrolled in Children's Hospital 1, Ho Chi Minh City, Vietnam and diagnoses, prescribed therapy and outcome were recorded on first visit and on follow-up after 7 days. Respiratory bacterial and viral pathogens were detected using molecular assays. Antibiotic use before presentation was assessed using questionnaires and urine HPLC. The impact of antibiotic usage on intestinal Enterobacteriaceae was assessed with semi-quantitative culture on agar with and without antibiotics on presentation and after 7 and 28 days. RESULTS: A total of 563 patients were enrolled between February 2009 and February 2010. Antibiotics were prescribed for all except 2 of 563 patients. The majority were 2nd and 3rd generation oral cephalosporins and amoxicillin with or without clavulanic acid. Respiratory viruses were detected in respiratory specimens of 72.5% of patients. Antibiotic use was considered inappropriate in 90.1% and 67.5%, based on guidelines and detected pathogens, respectively. On presentation parents reported antibiotic use for 22% of patients, 41% of parents did not know and 37% denied antibiotic use. Among these three groups, six commonly used antibiotics were detected with HPLC in patients' urine in 49%, 40% and 14%, respectively. Temporary selection of 3rd generation cephalosporin resistant intestinal Enterobacteriaceae during antibiotic use was observed, with co-selection of resistance to aminoglycosides and fluoroquinolones. CONCLUSIONS: We report overuse and overprescription of antibiotics for uncomplicated ARI with selection of resistant intestinal Enterobacteriaceae, posing a risk for community transmission and persistence in a setting of a highly granular healthcare system and unrestricted access to antibiotics through private pharmacies. REGISTRATION: This study was registered at the International Standard Randomised Controlled Trials Number registry under number ISRCTN32862422: http://www.isrctn.com/ISRCTN32862422