The effect of toxic Microcystis aeruginosa on four different populations of Daphnia

Cyanobacteria reduce the fitness of many Daphnia species, and blooms in eutrophic lakes may place strong selective pressure upon these primary consumers. This study examines the ability of daphnids to resist the deleterious effects of toxic Microcystis and determine if this resistance is related to the trophic conditions of their native lakes. Three populations of Daphnia pulex/pulicaria were examined; D. pulicaria from eutrophic Klamath Lake in Oregon, D. pulex from eutrophic Old Durham Reservoir in New Hampshire, and D. pulicaria from oligotrophic Russell Pond in New Hampshire. D. carinata from meso-oligotrophic Lake Rotoaira in New Zealand was used as a known cyanobacteria-sensitive species. Ten-day old 5th-6th instar animals were exposed to a mixture of Microcystis aeruginosa and Chlorella vulgaris (25% and 100% M. aeruginosa). Body length, lipid index, reproductive index and clearance rate were assessed for each population after 120 hours of treatment. A feeding bioassay response quantifying the energetic (feeding rate) cost of post abdominal rejections was also determined for a gradient of M. aeruginosa concentrations from 0% to 100%. The four populations of Daphnia exhibited different rates of decline in overall fitness when exposed to Microcystis. Populations exposed to Microcystis exhibited reduced thoracic beat rate, lower lipid and reproductive indexes, and higher cost of post abdominal rejections in comparison to daphnids in the control Chlorella. Length was not a sensitive indicator of fitness level. D. pulex from eutrophic Klamath Lake had a mean clearance rate in 100% Microcystis that was three to four times higher than D. pulicaria from oligotrophic Russell Pond. In general, daphnids from oligotrophic lakes exhibited a more drastic decline in fitness than daphnids from eutrophic lakes. This suggests that taxonomically related populations of Daphnia have evolved a suite of adaptations to Microcystis depending upon their history of exposure

Bioaccumulation of Microcystins by Freshwater Mussels in Mystic Lake and Middle Pond, MA

The UNH Center for Freshwater Biology investigated a possible relationship between a cyanobacteria bloom and a large-scale die-off of freshwater mussels in Mystic Lake and Middle Pond (Barnstable, MA). Four mussel species, Elliptio complanata (Eastern Elliptio), Pyganodon cataracta (Eastern Floater), Leptodea ochracea (Tidewater Mucket), and Lampsilis radiata (Eastern Lampmussel) (Nedeau, 2008), along with water samples, were collected from these lakes on August 9, 2010 (during bloom) and again on September 29 and October 8, 2010 (post-bloom). Hepatopancreas tissue, foot tissue, and water samples were tested for the cyanobacteria toxins, microcystins (MC), using ELISA techniques. MC concentrations in the hepatopancreas were generally higher (171.2 ng MC g-1 dry weight (dw)) than in the muscle (foot) tissue (55.8 ng MC g -1 dw) for each species. Average microcystin concentrations in mussels sampled during postbloom tissues were slightly lower (161.6 ng MC g-1 dw) than those collected during the cyanobacteria bloom (171.2 ng MC g1 dw). Live mussels were also subjected to a depuration experiment to determine the release of MC from mussels into the water. Mussels that were placed in cyanobacteria-free water depurated 61-90% MC within the first few days demonstrating their ability to release free MC-cyanotoxins into the lake water

Toxic Cyanobacteria Aerosols: Tests of Filters for Cells

Aerosolization of toxic cyanobacteria released from the surface of lakes is a new area of study that could uncover a previously unknown route of exposure to toxic cyanobacteria. Since toxic cyanobacteria may be responsible for adverse human health effects, methods and equipment need to be tested and established for monitoring these airborne bacteria. The primary focus of this study was to create controlled laboratory experiments that simulate natural lake aerosol production. I set out to test for the best type of filter to collect and analyze the aerosolized cells as small as 0.2-2.0 µm, known as picoplankton. To collect these aerosols, air was vacuumed from just above a sample of lake water passing through either glass fiber filters (GFF) or 0.22 µm MF-Millipore™ membrane filters (0.22 Millipore™). Filter collections were analyzed through epiflourescence microscopy for determining cell counts. Data analysis revealed that 0.22 Millipore™ filters were the best option for cell enumeration providing better epiflourescence optical quality and higher cell counts