Production and turnover of organic matter in three southern European Fagus sylvatica L. Stands

17 pages, 5 figures, 7 tables.[EN] Above-ground biomass, litterfall and litter accumulation and decomposition at the soil surface were studied within three Mediterranean beech forests from Italy, France and Spain in order to better understand the recycling of elements associated with the turnover organic matter Above-ground tree biomass amounted to 131.9 Mg ha'^ at Etna (Italy), 134.2 Mg ha'' at Sierra de la Demanda (Spain) and 223.9 Mg ha'' at Mont Lozère (France). The highest amount of total litterfall was observed at Sierra de la Demanda (4.7 Mg ha-' year'), followed by the Mont Lozère (4.4 Mg ha' year' ) and Etna (3.9 Mg ha' year'). Total organic matter accumulated on the soil surface in the three beech forests amounted to 25.8 Mg ha'' at Mont Lozère, 14.4 Mg ha'' at Sierra de la Demanda and 12.6 Mg ha'' at Etna. The relative proportions of leaf litter versus total litter were nearly the same in the Etna and Sierra de la Demanda forests (72 - 70%), and close to these values for Mont Lozère (65%). All the studied Mediterranean Fagus sylvatica stands appeared very similar concerning the organic matter distribution and fluxes, even if local climate and soil differences can be noticed.[FR] Les biomasses aériennes, les retombées de litières, leur accumulation à la surface du sol et leur décomposition, ont été étudiées dans trois hêtraies méditerranéennes d'Italie, de France et d'Espagne, pour mieux connaître la restitution au sol des bioéléments par l'intermédiaire de la matière organique. Les biomasses aériennes s'élevaient respectivement à 131.9 Mg ha-1 dans la hêtraie de l'Etna (Italie), 134.2 Mg ha-1 dans celle de Sierra de la Demanda (Espagne) et à 223.9 Mg ha-1 au Mont Lozère (France). Les retombées de litière les plus fortes sont observées dans la hêtraie de la Sierra de la Demanda (4.7 Mg ha-1 an-1), suivie de celles du Mont Lozère (4.4 Mg ha-1 an-1) et de l'Etna (3.9 Mg ha-1 an-1). L'ensemble de la matière organique accumulée à la surface du sol des trois hêtraies était de 25.8 Mg ha-1 au Mont Lozère, 14.4 Mg ha-1 à Sierra de la Demanda et 12.6 Mg ha-1 dans l'Etna. Les proportions respectives de la fraction feuilles par rapport à la litière totale étaient proches dans les stations de l'Etna et de la Sierra de la Demanda (72 - 70%) et voisines de celle du Mont Lozère (65%). Toutes les forêts de Fagus sylvatica dans le climat Méditerranéen sont très similaires par rapport à la distribution et aux flux, malgré que les conditions de climat et de sol soient différentes.[ES] Se han estudiado la biomasa aérea, la caída, acumulación y descomposición de la hojarasca en tres ecosistemas forestales de hayedo en Italia, Francia y España en orden a conocer mejor el reciclado de elementos- biógenos asociados al reciclaje de materia orgánica. La biomasa aérea estimada es de 131.0 Mg ha-1 en Etna (Italia), 134.2 Mg ha-1 en la Sierra de la Demanda (España) y 223.9 Mg ha-1 en Mont Lozère (Francia). La mayor cantidad de caída de hojarasca se ha observado en la Sierra de la Demanda (4.7 Mg ha-1 año-1) seguida de Mont Lozère (4.4 Mg ha-1 año-1). La materia orgánica acumulada en la superficie del suelo en los tres ecosistemas forestales estudiados fue de 25.8 Mg ha-1 en Mont Lozère, 14.4 Mg ha-1 en la Sierra de la Demanda y 12.6 Mg ha-1 en Etna. El porcentaje relativo de las hojas con relación a la hojarasca total fue similar en las parcelas de Etna y Sierra de la Demanda (72-70%) y algo más bajo en Mont Lozère (65%). Los bosques de Fagus sylvatica estudiados reflejaron valores similares respecto a la distribución y flujos de materia orgánica, aunque se han evidenciado diferencias en cuanto a condiciones locales de clima y suelo.This project has been financed by C. E. and I. N. I. A. We thank the facilities given to us by the Environmental Service of J. C. L. in Burgos, Montpellier and Catania and in the same way to the ground staff who have collaborated in it.Peer reviewe

De novo a-to-i rna editing discovery in lncrna

Background: Adenosine to inosine (A-to-I) RNA editing is the most frequent editing event in humans. It converts adenosine to inosine in double-stranded RNA regions (in coding and noncoding RNAs) through the action of the adenosine deaminase acting on RNA (ADAR) enzymes. Long non-coding RNAs, particularly abundant in the brain, account for a large fraction of the human transcriptome, and their important regulatory role is becoming progressively evident in both normal and transformed cells. Results: Herein, we present a bioinformatic analysis to generate a comprehensive inosinome picture in long non-coding RNAs (lncRNAs), using an ad hoc index and searching for de novo editing events in the normal brain cortex as well as in glioblastoma, a highly aggressive human brain cancer. We discovered >10,000 new sites and 335 novel lncRNAs that undergo editing, never reported before. We found a generalized downregulation of editing at multiple lncRNA sites in glioblastoma samples when compared to the normal brain cortex. Conclusion: Overall, our study discloses a novel layer of complexity that controls lncRNAs in the brain and brain cancer

Biological monitoring of occupational exposure to metals in electric steel foundry workers and its contribution to 8-oxo-7,8-dihydro-2′-deoxyguanosine levels

In this study, the urinary concentrations of selected metals in workers from an electric steel foundry in Tunisia were assessed and compared with existing biological limit values and general population reference values. Moreover, the association between oxidative DNA damage, measured as urinary 8-oxo-7,8-dihydro-2\u2019deoxyguanosine (8-oxodG) and co-exposure to metals and polycyclic aromatic hydrocarbons (PAHs) was evaluated. Urinary levels of 12 metals were determined by inductively coupled plasma-mass spectrometry (ICP-MS) in end-shift spot samples from 89 workers. The urinary levels of phenanthrene (U-PHE), as marker of exposure to PAHs, and 8-oxodG were also available. Median levels ranged from 0.4 \ub5g/L (cobalt, Co, and thallium, Tl) to 895 \ub5g/L (zinc, Zn). Only 1% of samples was above the biological limit values for Co, and up to 13.5% of samples were above limit values for Cd. From 3.4% (Co) to 72% (lead, Pb) of samples were above the reference values for the general population. Multiple linear regression models, showed that manganese (Mn), Zn, arsenic (As), barium (Ba), Tl, and Pb were significant predictors of 8-oxodG (0.012 64 p 64 0.048); U-PHE was also a significant predictor (0.003 64 p 64 0.059). The variance explained by models was low (0.11 64 R2 64 0.17, p < 0.005), showing that metals and PAHs were minor contributors to 8-oxodG. Overall, the comparison with biological limit values showed that the study subjects were occupationally exposed to metals, with levels exceeding biological limit values only for Cd

Métodos alternativos de muestreo para estimar la estructura y caracteres reproductivos de bosques

Aleppo pine had a large forest cover in North Africa and along the Mediterranean basin which management is not fully developed and new forest tools are required. In this research, the forest structure, epidometric characteristics and allometric relationships were studied in 79 plots covering four bioclimatic zones from natural even aged forests of Aleppo pine located between eastern Algeria to the western coastal part of Tunisia. To characterize the forest structure three sampling methods were carried out: classical inventories (recording all pine trees), and two simplified approach using one average size or five dominant pine tree per plot. Annual growth increment and cone production were only calculated for the average size tree. Furthermore, the analysis of variance showed non-significant differences recorded between bioclimatic zones in trunk or crown diameter using the two simplified approach. Moreover, a significant decrease from wetter to drier areas in total height, crown height, cone seed production was observed only for average size tree method. However, the analysis of covariance showed significant differences between both approaches in total height, trunk diameter and crown coverage which were largely influenced by the pine tree density. In future investigations, we confirmed previous research that the dominant tree is a good sampling method to examine the site fertility, whereas the average size tree constitutes a valuable approach to study the population growth and reproduction.El pino carrasco muestra una gran cobertura forestal, tanto en el norte de África como en toda la Cuenca Mediterránea, cuyo manejo no está totalmente desarrollado lo que requiere nuevas herramientas de manejo. En esta investigación, se estudiaron la estructura forestal, las características epidométricas y las relaciones alométricas de 79 parcelas distribuidas a lo largo de cuatro zonas bioclimáticas en bosques regulares de pino carrasco situados entre el este de Argelia y la costa este de Túnez. Para caracterizar la estructura forestal se aplicaron tres métodos de muestreo: inventario clásico (midiendo todos los pies) y dos propuestas más sencillas, usando un árbol medio o cinco pies dominantes por parcela. El análisis de varianza no mostraba diferencias significativas de las mediciones de diámetro de tronco y de copa realizadas entre las distintas zonas bioclimáticas usando ambos métodos. Por otra parte, se observó una disminución significativa de las zonas más húmedas a las más secas en altura toral, altura de copa y producción de semillas fue observada aplicando el método de árbol medio. Además, el análisis de covarianzas mostraba diferencias significativas, usando ambas metodologías, en los valores medios de altura total, diámetro de tronco y cobertura de copa que mostraron estar influenciados por los valores de la densidad de arbolado de cada zona. Para futuras investigaciones, confirmamos estudios previos que apuntaban que la propuesta de muestreo de árboles dominantes como un buen método de muestreo para caracterizar la calidad de sitio mientras que la de árbol medio constituye una valiosa propuesta para el estudio del crecimiento de poblaciones y sus características reproductivas

Production and turnover of organic matter in three southern European Fagus sylvatica L. Stands

17 pages, 5 figures, 7 tables.[EN] Above-ground biomass, litterfall and litter accumulation and decomposition at the soil surface were studied within three Mediterranean beech forests from Italy, France and Spain in order to better understand the recycling of elements associated with the turnover organic matter Above-ground tree biomass amounted to 131.9 Mg ha'^ at Etna (Italy), 134.2 Mg ha'' at Sierra de la Demanda (Spain) and 223.9 Mg ha'' at Mont Lozère (France). The highest amount of total litterfall was observed at Sierra de la Demanda (4.7 Mg ha-' year'), followed by the Mont Lozère (4.4 Mg ha' year' ) and Etna (3.9 Mg ha' year'). Total organic matter accumulated on the soil surface in the three beech forests amounted to 25.8 Mg ha'' at Mont Lozère, 14.4 Mg ha'' at Sierra de la Demanda and 12.6 Mg ha'' at Etna. The relative proportions of leaf litter versus total litter were nearly the same in the Etna and Sierra de la Demanda forests (72 - 70%), and close to these values for Mont Lozère (65%). All the studied Mediterranean Fagus sylvatica stands appeared very similar concerning the organic matter distribution and fluxes, even if local climate and soil differences can be noticed.[FR] Les biomasses aériennes, les retombées de litières, leur accumulation à la surface du sol et leur décomposition, ont été étudiées dans trois hêtraies méditerranéennes d'Italie, de France et d'Espagne, pour mieux connaître la restitution au sol des bioéléments par l'intermédiaire de la matière organique. Les biomasses aériennes s'élevaient respectivement à 131.9 Mg ha-1 dans la hêtraie de l'Etna (Italie), 134.2 Mg ha-1 dans celle de Sierra de la Demanda (Espagne) et à 223.9 Mg ha-1 au Mont Lozère (France). Les retombées de litière les plus fortes sont observées dans la hêtraie de la Sierra de la Demanda (4.7 Mg ha-1 an-1), suivie de celles du Mont Lozère (4.4 Mg ha-1 an-1) et de l'Etna (3.9 Mg ha-1 an-1). L'ensemble de la matière organique accumulée à la surface du sol des trois hêtraies était de 25.8 Mg ha-1 au Mont Lozère, 14.4 Mg ha-1 à Sierra de la Demanda et 12.6 Mg ha-1 dans l'Etna. Les proportions respectives de la fraction feuilles par rapport à la litière totale étaient proches dans les stations de l'Etna et de la Sierra de la Demanda (72 - 70%) et voisines de celle du Mont Lozère (65%). Toutes les forêts de Fagus sylvatica dans le climat Méditerranéen sont très similaires par rapport à la distribution et aux flux, malgré que les conditions de climat et de sol soient différentes.[ES] Se han estudiado la biomasa aérea, la caída, acumulación y descomposición de la hojarasca en tres ecosistemas forestales de hayedo en Italia, Francia y España en orden a conocer mejor el reciclado de elementos- biógenos asociados al reciclaje de materia orgánica. La biomasa aérea estimada es de 131.0 Mg ha-1 en Etna (Italia), 134.2 Mg ha-1 en la Sierra de la Demanda (España) y 223.9 Mg ha-1 en Mont Lozère (Francia). La mayor cantidad de caída de hojarasca se ha observado en la Sierra de la Demanda (4.7 Mg ha-1 año-1) seguida de Mont Lozère (4.4 Mg ha-1 año-1). La materia orgánica acumulada en la superficie del suelo en los tres ecosistemas forestales estudiados fue de 25.8 Mg ha-1 en Mont Lozère, 14.4 Mg ha-1 en la Sierra de la Demanda y 12.6 Mg ha-1 en Etna. El porcentaje relativo de las hojas con relación a la hojarasca total fue similar en las parcelas de Etna y Sierra de la Demanda (72-70%) y algo más bajo en Mont Lozère (65%). Los bosques de Fagus sylvatica estudiados reflejaron valores similares respecto a la distribución y flujos de materia orgánica, aunque se han evidenciado diferencias en cuanto a condiciones locales de clima y suelo.This project has been financed by C. E. and I. N. I. A. We thank the facilities given to us by the Environmental Service of J. C. L. in Burgos, Montpellier and Catania and in the same way to the ground staff who have collaborated in it.Peer reviewe

Forced convection and thermocapillarity in silicon Czochraslki crystal growth in hemispherical crucible

International audienceIn order to understand the influence of a semispherical crucible geometry combined with different convection modes as a thermocapillary convection, natural convection and forced convection, induced by crystal rotation, on melt flow pattern in silicon Czochralski crystal growth process, a set of numerical simulations are conducted using Fluent Software. We solve the system of equations of heat and momentum transfer in classical geometry as cylindrical and modified crystal growth process geometry as cylindro-spherical. In addition, we adopt hypothesis adapted to boundary conditions near the interface and calculations are executed to determine temperature, pressure and velocity fields versus Grashof and Reynolds numbers. The analysis of the obtained results led to conclude that there is advantage to modify geometry in comparison with the traditional one. The absence of the stagnation regions of fluid in the hemispherical crucible corner and the possibility to control the melt flow using the crystal rotation enhances the quality of the process comparatively to the cylindrical one. The pressure field is strongly related to the swirl velocity