Conditional protein degradation in Yarrowia lipolytica using the auxin-inducible degron

Conditional protein degradation is a powerful tool for controlled protein knockdown. The auxin-inducible degron (AID) technology uses a plant auxin to induce depletion of degron-tagged proteins, and it has been shown to be functional in several non-plant eukaryotes. In this study, we demonstrated AID-based protein knockdown in an industrially important oleaginous yeas

Polymer-based microring resonator with the multimode interference coupler operating at very-near-infrared wavelengths

A microring resonator with the multimode interference coupler is fabricated on the polymer platform by using UV-based soft nanoimprint technique. A unique class of fluorinated polymer, perfluoropolyether (PFPE), is employed for the fabrication of the flexible soft mold. By optimizing the proportion between Ormocore and the thinner maT, the microring resonator is fabricated almost without residual layer. The fabricated device with a Q-factor up to 2.3 × 104 is demonstrated for very-near-infrared wavelengths, which shows high potential for sensing applications

Tilted fiber bragg grating sensor using chemical plating of a palladium membrane for the detection of hydrogen leakage

A tilted fiber Bragg grating (TFBG) hydrogen sensor coated with a palladium (Pd) membrane by the electroless plating method is proposed in this paper. A uniform layer of Pd metal is fabricated in aqueous solutions by the chemical coating method, which is used as the sensitive element to detect the change of the surrounding refractive index (SRI) caused by hydrogen absorption. The change in SRI causes an unsynchronized change of the cladding modes and the Bragg peak in the TFBG transmission spectrum, thereby eliminating the cross-sensitivity due to membrane expansion and is able to simultaneously monitor the presence of cracks in the pipe, as well as the hydrogen leakage. By subtracting the wavelength shift caused by fiber expansion, the change of SRI, i.e., the information from the H2 level, can be separately obtained. The drifted wavelength is measured for the H2 concentration below the hydrogen explosion limit between 1% and 4%. The chemical-based coating has the advantages of a low cost, a simple operation, and being suitable for coating on long fiber structures. The proposed sensor is able to detect the H2 signal in 5 min at a 1% H2 concentration. The proposed sensor is proved to be able to monitor the hydrogen level without the cross-sensitivity of temperature variation and expansion strains, so could be a good candidate for security applications in industr

RF Characterization of Self-Interference Cancellation Using Phase Modulation and Optical SideBand Filtering

Full-Duplex scheme transmitting and receiving signals simultaneously in the same frequency band can significantly improve the throughput and the spectrum efficiency, and is considered as a candidate technology for the fifth generation (5G) wireless communication. However, the high power transmitted signal will interfere with the in-band weak received signal, which is called as RF self-interference. It cannot be simply removed by a notch filter or a narrow bandpass filter because the same frequency band is used for both transmitter and receiver. An optical approach to implement RF self-interference cancellation is proposed. Based on the inherent out-of-phase property between the left and right sidebands of phase-modulated signal and optical sideband filtering, the RF self-interference cancellation is achieved by tuning the delay time and amplitude in the optical domain. The cancellation depth of the system was measured for different frequencies and bandwidths. The cancellation performance affected by the time delay deviation, the amplitude deviation and phase response is analyzed according to experimental results. It gives the direction for the improvement of system performance. Finally, the full-duplex communication by using the optical SIC approach was also investigated. Signal of interest is recovered and the constellation diagram was also shown

Optical Multipath RF Self-Interference Cancellation Based on Phase Modulation for Full-Duplex Communication

Optical multipath RF self-interference cancellation (SIC) based on phase modulation for full-duplex communication is proposed and demonstrated experimentally. Phase modulation is utilized to convert the RF signal into optical domain, in which the time delay tuning, amplitude tuning and phase inversion for multipath RF SIC are completed. The comprehensive theoretical model of the optical multipath RF SIC system is established, and the factors affecting SIC performance including the time delay, amplitude and phase deviations are analyzed. The experimental results verify the feasibility of the proposed scheme for full-duplex communication with the cancellation depth of 26 dB and 28 dB over 100 MHz at central frequency of 6 GHz and 10 GHz, respectively. A figure of merit of the maximum interference to signal of interest ratio is defined to characterize the SOI recovery capability of optical RF SIC system

Analysis of Yarrowia lipolytica Growth, Catabolism, and Terpenoid Biosynthesis during Utilization of Lipid-derived Feedstock

This study employs biomass growth analyses and 13C-isotope tracing to investigate lipid feedstock utilization by Yarrowia lipolytica. Compared to glucose, oil-feedstock in the minimal medium increases the yeast\u27s biomass yields and cell sizes, but decreases its protein content (\u3c20% of total biomass) and enzyme abundances for product synthesis. Labeling results indicate a segregated metabolic network (the glycolysis vs. the TCA cycle) during co-catabolism of sugars (glucose or glycerol) with fatty acid substrates, which facilitates resource allocations for biosynthesis without catabolite repressions. This study has also examined the performance of a β-carotene producing strain in different growth mediums. Canola oil-containing yeast-peptone (YP) has resulted in the best β-carotene titer (121 ± 13 mg/L), two-fold higher than the glucose based YP medium. These results highlight the potential of Y. lipolytica for the valorization of waste-derived lipid feedstock

Performance analysis of photonic RF self-interference cancellation for full-duplex communication

A photonic RF self-interference cancellation (SIC) scheme for full-duplex communication is proposed and demonstrated experimentally. It is based on phase modulation to convert the RF signal into optical domain. The interference cancellation performance of the photonic RF SIC system under different delay deviation (Δτ) and amplitude deviation (Δα) is analyzed. The cancellation depth of 34.5 dB is measured for 10 GHz signal with bandwidth of 50MHz. According to experimental results, the interference cancellation performance affected by the time delay deviation, the amplitude deviation and the phase response is investigated. The results give a direction for the improvement of system performance