Investigation of the Scanning Microarc Oxidation Process

Scanning microarc oxidation (SMAO) is a coating process which is based on conventional microarc oxidation (MAO). The key difference is that deposition in SMAO is achieved by using a stainless steel nozzle to spray an electrolyte stream on the substrate surface as opposed to immersing the workpiece in an electrolyzer. In the present study, SMAO discharge characteristics, coating morphology, and properties are analyzed and compared to results obtained from MAO under similar conditions. Results show that MAO and SMAO have comparable spark and microarc lifetimes and sizes, though significant differences in incubation time and discharge distribution were evident. Results also showed that the voltage and current density for MAO and SMAO demonstrate similar behavior but have markedly different transient and steady-state values. Results obtained from coating A356 aluminum sheet show that oxide thickness and growth rate in SMAO are strongly dependent on interelectrode spacing and travel speed. Analysis of the SMAO coating morphology and structure showed that a denser and slightly harder layer was deposited in comparison to MAO and is attributed to reduced porosity and increased formation of α-Al2O3. Preliminary results indicate that SMAO represents a viable process for coating of aluminum surfaces

Effect of Ca/P Ratio on Bioactivity of PEO Coatings

Ti and its alloys are used in biomedical applications because of their low density, high specific strength, and high biocompatibility. However, the poor bioactivity limits their wide application. Herein, ceramic coatings containing Ca and P were prepared by plasma electrolytic oxidation（PEO） on Ti6Al4V alloy, and the microstructure, surface morphology, and chemical composition of these coatings were evaluated. An effect of the different Ca/P ratios in the electrolyte on the CaTiO3-forming ability was investigated; besides, the effect of different Ca/P ratios in these coatings on the hydroxyapatite-inducing potency was also investigated. In addition, the bioactivity of the coatings was compared between before and after the immersion in a simulated body fluid. It was found that the higher the Ca/P ratio in the electrolyte, the stronger the CaTiO3-forming ability, and the higher the Ca/P ratio in the coating, the better the bioactivity of the coatings

Uncovering the pharmacological mechanisms of Zizhu ointment against diabetic ulcer by integrating network analysis and experimental evaluation in vivo and in vitro

Diabetic ulcer (DU) has been recognized as one of the most prevalent and serious complications of diabetes. However, the clinical efficacy of standard treatments for DU remains poor. Traditional Chinese medicine (TCM) shows a positive therapeutic effect on DU. Specifically, Zizhu ointment (ZZO) has been widely used to treat DU in long-term clinical practice, but the exact mechanism by which it promotes DU wound healing remains unknown. In this study, network analysis and high-performance liquid chromatography–high resolution mass spectrometry (UPLC-HRMS) were conducted to identify the active compounds of ZZO. We detected isovalerylshikonin (ISO), mandenol, daidzein, kaempferol, and formononetin in both network analysis and UPLC-HRMS. Moreover, ZZO could ameliorate DU by regulating the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT) and inflammation signaling pathways, according to the results of KEGG analysis. We established a DU mouse model with a high-fat diet and streptozotocin injection in vivo to evaluate the network analysis result. The experimental results showed that ZZO could inhibit inflammation, remodel fibrous tissue, and promote angiogenesis in the DU area, facilitating wound healing in DU mice. Moreover, the PI3K/AKT signaling pathway was indeed activated by ZZO treatment, promoting macrophage M2 polarization. In addition, we used molecular docking technology to evaluate the binding sites between ZZO and the PI3K/AKT pathway. The results showed that ISO has a good binding interaction with AKT. Moreover, ISO promoted M2 polarization in macrophages in a dose-dependent manner in vitro. Our study found that ZZO could promote DU wound healing by inhibiting inflammation, which was achieved by macrophage M2 polarization through activating the PI3K/AKT pathway. Further studies have demonstrated that ISO plays major role in the above process. These findings provide a theoretical basis for further preclinical evaluation and lay a foundation for nano-gel compound treatment with ZZO

An Assessment of Multimodel Simulations for the Variability of Western North Pacific Tropical Cyclones and Its Association with ENSO

An assessment of simulations of the interannual variability of tropical cyclones (TCs) over the western North Pacific (WNP) and its association with El Niño–Southern Oscillation (ENSO), as well as a subsequent diagnosis for possible causes of model biases generated from simulated large-scale climate conditions, are documented in the paper. The model experiments are carried out by the Hurricane Work Group under the U.S. Climate Variability and Predictability Research Program (CLIVAR) using five global climate models (GCMs) with a total of 16 ensemble members forced by the observed sea surface temperature and spanning the 28-yr period from 1982 to 2009. The results show GISS and GFDL model ensemble means best simulate the interannual variability of TCs, and the multimodel ensemble mean (MME) follows. Also, the MME has the closest climate mean annual number of WNP TCs and the smallest root-mean-square error to the observation. Most GCMs can simulate the interannual variability of WNP TCs well, with stronger TC activities during two types of El Niño—namely, eastern Pacific (EP) and central Pacific (CP) El Niño—and weaker activity during La Niña. However, none of the models capture the differences in TC activity between EP and CP El Niño as are shown in observations. The inability of models to distinguish the differences in TC activities between the two types of El Niño events may be due to the bias of the models in response to the shift of tropical heating associated with CP El Niño

Antiretroviral Therapy, Renal Function among HIV-Infected Tanzanian, Adults, HIV/AIDS, .

Data regarding the outcomes of HIV-infected adults with baseline renal dysfunction who start antiretroviral therapy are conflicting. We followed up a previously-published cohort of HIV-infected adult outpatients in northwest Tanzania who had high prevalence of renal dysfunction at the time of starting antiretroviral therapy (between November 2009 and February 2010). Patients had serum creatinine, proteinuria, microalbuminuria, and CD4(+) T-cell count measured at the time of antiretroviral therapy initiation and at follow-up. We used the adjusted Cockroft-Gault equation to calculate estimated glomerular filtration rates (eGFRs). In this cohort of 171 adults who had taken antiretroviral therapy for a median of two years, the prevalence of renal dysfunction (eGFR <90 mL/min/1.73 m(2)) decreased from 131/171 (76.6%) at the time of ART initiation to 50/171 (29.2%) at the time of follow-up (p<0.001). Moderate dysfunction (eGFR<60 mL/min/1.73 m(2)) decreased from 21.1% at antiretroviral therapy initiation to 1.1% at follow-up (p<0.001), as did the prevalence of microalbuminuria (72% to 44%, p<0.001). Use of tenofovir was not associated with renal dysfunction at follow-up. Mild and moderate renal dysfunction were common in this cohort of HIV-infected adults initiating antiretroviral therapy, and both significantly improved after a median follow-up time of 2 years. Our work supports the renal safety of antiretroviral therapy in African adults with mild-moderate renal dysfunction, suggesting that these regimens do not lead to renal damage in the majority of patients and that they may even improve renal function in patients with mild to moderate renal dysfunction

13C pulse-chase labeling comparative assessment of the active methanogenic archaeal community composition in the transgenic and nontransgenic parental rice rhizospheres

Rhabdosargus holubi (Steindachner, 1881) is a small (maximum size = 450 mm total length; Heemstra and Heemstra 2004) sparid that is distributed along the south-east coast of Africa from St Helena Bay, South Africa, to Maputo, Mozambique (Götz and Cowley 2013). Spawning occurs in the nearshore marine environment primarily during winter, specifically May–August in KwaZulu-Natal (KZN) (Wallace 1975) and July–February in the South-Eastern Cape (Whitfield 1998). Individuals reach 50% sexual maturity at approximately 150 mm standard length (SL) in the Eastern Cape (Whitfield 1998). The early life stages are transported by the south-westward-flowing Agulhas Current, and recruit as post-flexion larvae and early juveniles into estuaries during late winter and early summer (Blaber 1974). The warm temperatures and high nutrient levels in estuaries favour fast growth (Blaber 1973a), and fish spend their first year of life in these environments, migrating back out to sea after reaching approximately 120 mm SL. Some individuals remain trapped in closed estuaries, where they may reach sizes greater than 200 mm SL (James et al. 2007a). Rhabdosargus holubi is the dominant estuarine-dependent marine teleost species recorded in permanently open and temporarily open/closed estuaries in the warm-temperate region, which spans the south, south-east and east coast of South Africa (Harrison 2005). The species is also an important component of the linefishery in many SouthAfrican estuaries (10–15.6% by number) (Pradervand and Baird 2002), particularly in Eastern Cape estuaries (Cowley et al. 2003). These figures underestimate the presence of R. holubi, as most individuals making use of estuaries are young, feeding predominately on filamentous macroalgae and diatom flora, and are generally too small to be caught with hook and line (De Wet and Marais 1990). James et al. (2007b) showed that R. holubi made up 34–92% of the annual seine-net catch in the East Kleinemonde Estuary. Rhabdosargus holubi is also important in the KZN shorebased linefishery, representing 4.6% of the total landed catch (Dunlop and Mann 2012)More and more investigations indicate that genetic modification has no significant or persistent effects on microbial community composition in the rice rhizosphere. Very few studies, however, have focused on its impact on functional microorganisms. This study completed a 13C-CO2 pulse-chase labeling experiment comparing the potential effects of cry1Ab gene transformation on 13C tissue distribution and rhizosphere methanogenic archaeal community composition with its parental rice variety (Ck) and a distant parental rice variety (Dp). Results showed that 13C partitioning in aboveground biomass (mainly in stems) and roots of Dp was significantly lower than that of Ck. However, there were no significant differences in 13C partitioning between the Bt transgenic rice line (Bt) and Ck. RNA-stable isotope probing combined with clone library analyses inferred that the group Methanosaetaceae was the predominant methanogenic Archaea in all three rice rhizospheres. The active methanogenic archaeal community in the Bt rhizosphere was dominated by Methanosarcinaceae, Methanosaetaceae, and Methanomicrobiaceae, while there were only two main methanogenic clusters (Methanosaetaceae and Methanomicrobiaceae) in the Ck and Dp rhizospheres. These results indicate that the insertion of cry1Ab gene into the rice genome has the potential to result in the modification of methanogenic community composition in its rhizosphere

Expert Consensus on Microtransplant for Acute Myeloid Leukemia in Elderly Patients -Report From the International Microtransplant Interest Group

Recent studies have shown that microtransplant (MST) could improve outcome of patients with elderly acute myeloid leukemia (EAML). To further standardize the MST therapy and improve outcomes in EAML patients, based on analysis of the literature on MST, especially MST with EAML from January 1st, 2011 to November 30th, 2022, the International Microtransplant Interest Group provides recommendations and considerations for MST in the treatment of EAML. Four major issues related to MST for treating EAML were addressed: therapeutic principle of MST (1), candidates for MST (2), induction chemotherapy regimens (3), and post-remission therapy based on MST (4). Others included donor screening, infusion of donor cells, laboratory examinations, and complications of treatment

Present Geothermal Field and Roadway Heat Damage Characteristics in Zhujidong Coal Mine Area of Huainan City, Anhui Province, China

With the normalization of deep mining, the problem of mine thermal damage has been widely concerned. In this paper, 64 logging temperature data of surface boreholes of Zhujidong coalfield in Huainan City, Anhui Province, China, were summarized and sorted. The temperature data of 12 measuring points at the current main working level of −906 m and −965 m were measured. The ground temperature conditions of the vertical direction, horizontal direction, and main coal seam floor in the coalfield were analyzed. The characteristics of the present geothermal field and roadway heat damage, and its influencing factors were discussed. The results showed that the geothermal gradients of this area are between 1.7 ℃/hm and 3.6 ℃/hm, and the average geothermal gradient is 2.607 ℃/hm. It shows the transmission of warming in the vertical direction and horizontal direction shows the ground temperature lowering from south to north, east to west. The geothermal field in this area is mainly affected by the geological structure, and the thermal physical properties of rocks, magmatic rocks and groundwater activities also have a certain effect. At present, most of the main work level has reached the first heat hazard area, and some are in the second heat hazard area, according to Chinese regulations. The working face temperature is keeping above 28 7 ℃ and the relative humidity is being maintained above 70% for a long time. The problem of heat damage is extremely serious. It is suggested to construct the thermal environment control measures combining active and passive ways

Knowledge Graph Representation of Multi-Source Urban Storm Surge Hazard Information Based on Spatio-Temporal Coding and the Hazard Events Ontology Model

Coastal cities are increasingly vulnerable to urban storm surge hazards and the secondary hazards they cause (e.g., coastal flooding). Accurate representation of the spatio-temporal process of hazard event development is essential for effective emergency response. However, current knowledge graph representations face the challenge of integrating multi-source information with various spatial and temporal scales. To address this challenge, we propose a new information model for storm surge hazard events, involving a two-step process. First, a hazard event ontology is designed to model the components and hierarchical relationships of hazard event information. Second, we utilize multi-scale time segment integer coding and geographical coordinate subdividing grid coding to create a spatio-temporal framework, for modeling spatio-temporal features and spatio-temporal relationships. Using the 2018 typhoon Mangkhut storm surge event in Shenzhen as a case study and the hazard event information model as a schema layer, a storm surge event knowledge graph is constructed, demonstrating the integration and formal representation of heterogeneous hazard event information and enabling the fast retrieval of disasters in a given spatial or temporal range

Experimental study on creep characteristics of glazed hollow beads-cement/sodium silicate grouting materials

This study introduces a new thermal insulation grouting material designed for high-temperature mine environment. The thermal insulation effect of cement/sodium silicate grouting materials can be enhanced by adding glazed hollow beads with outstanding thermal insulation properties. The ratio with the best comprehensive performance was selected through orthogonal tests, and the long-term creep mechanical characteristics of glazed hollow beads-cement/sodium silicate grouting materials were analyzed. Graded equal incremental loading with different loading durations was used to test the specimens' instantaneous strain, creep strain, creep rate, critical stress, and microscopic structures. For each loading stage, the Burgers model was used to fit the creep curve. The results show that graded loading intensity surpasses uniaxial loading across different loading durations. The different loading durations significantly affect the creep parameters, and stress concentration damage initially manifests on the surface of glazed hollow beads. Finally, the theoretical curve obtained by the Burgers model fits well with the creep test curve. This study has good practical significance for high-temperature roadway thermal grouting insulation technology