Lineage of origin in rhabdomyosarcoma informs pharmacological response

Lineage or cell of origin of cancers is often unknown and thus is not a consideration in therapeutic approaches. Alveolar rhabdomyosarcoma (aRMS) is an aggressive childhood cancer for which the cell of origin remains debated. We used conditional genetic mouse models of aRMS to activate the pathognomonic Pax3:Foxo1 fusion oncogene and inactivate p53 in several stages of prenatal and postnatal muscle development. We reveal that lineage of origin significantly influences tumor histomorphology and sensitivity to targeted therapeutics. Furthermore, we uncovered differential transcriptional regulation of the Pax3:Foxo1 locus by tumor lineage of origin, which led us to identify the histone deacetylase inhibitor entinostat as a pharmacological agent for the potential conversion of Pax3:Foxo1-positive aRMS to a state akin to fusion-negative RMS through direct transcriptional suppression of Pax3:Foxo1.Stem Cell and Regenerative Biolog

Hydrometeor classification of quasi-vertical profiles of polarimetric radar measurements using a top-down iterative hierarchical clustering method

Correct, timely and meaningful interpretation of polarimetric weather radar observations requires an accurate understanding of hydrometeors and their associated microphysical processes along with well-developed techniques that automatize their recognition in both the spatial and temporal dimensions of the data. This study presents a novel technique for identifying different types of hydrometeors from quasi-vertical profiles (QVPs). In this new technique, the hydrometeor types are identified as clusters belonging to a hierarchical structure. The number of different hydrometeor types in the data is not predefined, and the method obtains the optimal number of clusters through a recursive process. The optimal clustering is then used to label the original data. Initial results using observations from the National Centre for Atmospheric Science (NCAS) X-band dual-polarization Doppler weather radar (NXPol) show that the technique provides stable and consistent results. Comparison with available airborne in situ measurements also indicates the value of this novel method for providing a physical delineation of radar observations. Although this demonstration uses NXPol data, the technique is generally applicable to similar multivariate data from other radar observations

Semantic Web or Web of Data? A diachronic study (1999 to 2017) of the publications of Tim Berners-Lee and the World Wide Web Consortium

The web has been, in the last decades, the place where information retrieval achieved its maximum importance, given its ubiquity and the sheer volume of information. However, its exponential growth made the retrieval task increasingly hard, relying in its effectiveness on idiosyncratic and somewhat biased ranking algorithms. To deal with this problem, a “new” web, called the Semantic Web (SW), was proposed, bringing along concepts like “Web of Data” and “Linked Data,” although the definitions and connections among these concepts are often unclear. Based on a qualitative approach built over a literature review, a definition of SW is presented, discussing the related concepts sometimes used as synonyms. It concludes that the SW is a comprehensive and ambitious construct that includes the great purpose of making the web a global database. It also follows the specifications developed and/or associated with its operationalization and the necessary procedures for the connection of data in an open format on the web. The goals of this comprehensive SW are the union of two outcomes still tenuously connected: the virtually unlimited possibility of connections between data—the web domain—with the potentiality of the automated inference of “intelligent” systems—the semantic component.1011-A149-0360 | Luís Miguel Oliveira Machadoinfo:eu-repo/semantics/publishedVersio