The Chemical Distribution in a Subluminous Type Ia Supernova: Hubble Space Telescope Images of the SN 1885 Remnant

SN 1885 was a probable subluminous SN Ia that occurred in the bulge of the Andromeda galaxy, M31, at a projected location 16\u27\u27 from the nucleus. Here we present and analyze Hubble Space Telescope images of the SN 1885 remnant seen in absorption against the M31 bulge via the resonance lines of Ca I, Ca II, Fe I, and Fe II. Viewed in Ca II H and K line absorption, the remnant appears as a nearly black circular spot with an outermost angular radius of 0.40\u27\u27 ± 0.025\u27\u27, implying a maximum linear radius of 1.52 ± 0.15 pc at M31\u27s estimated distance of 785 ± 30 kpc and hence a 120 yr average expansion velocity of 12,400 ± 1400 km s-1. The strongest Ca II absorption is organized in a broken ring structure with a radius of 0.2\u27\u27 (=6000 km s-1) with several apparent absorption clumps of an angular size around that of the image pixel scale of 0.05\u27\u27 (=1500 km s-1). Ca I and Fe I absorption structures appear similar except for a small Fe I absorption peak displaced 0.1\u27\u27 off-center of the Ca II structure by a projected velocity of about 3000 km s-1. Analyses of these images using off-center, delayed-detonation models suggest a low 56Ni production similar to the subluminous SN Ia explosion of SN 1986G. The strongly lopsided images of Ca I and Fe I can be understood as resulting from an aspherical chemical distribution, with the best agreement found using an off-center model viewed from an inclination of ~60°. The images require a central region of no or little Ca but with iron group elements indicative for burning under sufficiently high densities for electron capture to take place, i.e., burning prior to a significant preexpansion of the WD

Methods for Running a Successful Women-in-STEM Organization on an Academic Campus

The current academic culture facing women in science, technology, engineering, and math (STEM) fields in the United States has sparked the formation of grassroots advocacy groups to empower female scientists-in-training. However, the impact of these initiatives often goes unmeasured and underappreciated. Our Women in Science and Engineering (WiSE) organization serves post-doctoral researchers, graduate students, and research technicians (trainees) at a private research institute for biological sciences. Here we propose the following guidelines for cultivating a successful women-in-STEM-focused group based upon survey results from our own scientific community as well as the experience of our WiSE group leaders. We hope these recommendations can provide guidance to advocacy groups at other research and academic organizations that wish to strengthen their efforts. While our own group specifically focuses on the underrepresented state of women in science, we hope these guidelines may be adapted and applied to groups that advocate for any minority group within the greater scientific community (i.e. those of gender, race/ethnicity, socioeconomic background, sexual orientation, etc.)

piRNA-directed cleavage of meiotic transcripts regulates spermatogenesis.

MIWI catalytic activity is required for spermatogenesis, indicating that piRNA-guided cleavage is critical for germ cell development. To identify meiotic piRNA targets, we augmented the mouse piRNA repertoire by introducing a human meiotic piRNA cluster. This triggered a spermatogenesis defect by inappropriately targeting the piRNA machinery to mouse mRNAs essential for germ cell development. Analysis of such de novo targets revealed a signature for pachytene piRNA target recognition. This enabled identification of both transposable elements and meiotically expressed protein-coding genes as targets of native piRNAs. Cleavage of genic targets began at the pachytene stage and resulted in progressive repression through meiosis, driven at least in part via the ping-pong cycle. Our data support the idea that meiotic piRNA populations must be strongly selected to enable successful spermatogenesis, both driving the response away from essential genes and directing the pathway toward mRNA targets that are regulated by small RNAs in meiotic cells.This work was supported by the National Institutes of Health R37 grant GM062534-14 to G.J.H. iTRAQ was performed with assistance from the Cold Spring Harbor Laboratory Proteomics Shared Resource, which is supported by Cancer Center support grant 5P30CA045508. W.S.S.G. is a McClintock Fellow of the Watson School of Biological Sciences and is supported by the NSS Scholarship from the Agency for Science, Technology and Research, Singapore. O.H.T. is supported by a fellowship of the Human Frontier Science Program. R.B. is supported by the Starr Centennial Scholarship from the Watson School of Biological Sciences. G.J.H. is a Howard Hughes Medical Institute Investigator.This is the final version of the article. It first appeared from Cold Spring Harbor Laboratory Press via http://dx.doi.org/10.1101/gad.260455.11

Environmental Stewardship: Reducing Food Waste While Improving Food Security

Report, poster, project brief and presentation completed by students participating in the Economic Development Fellowship Consulting Program, offered through the Office of University Economic Development in Spring 2019.This project was completed as part of the 2018-2019 Resilient Communities Project (rcp.umn.edu) partnership with Ramsey County. In 2015, 12.7% of the population in Ramsey County did not have consistent access to enough food for an active and healthy life, or had limited or uncertain availability of nutritionally adequate food. At the same time, food remains the largest source of solid waste by weight in Ramsey County, accounting for 26% of the total trash collected for disposal each year. Ramsey County project lead Rae Eden Frank worked with students participating in the Economic Development Fellowship Consulting Program to investigate strategies for reducing food waste and diverting edible food to sources where it can be distributed for human consumption. The students’ final report and presentation, and a poster and project brief summarizing the project, are available.This project was supported by the Resilient Communities Project (RCP), a program at the University of Minnesota whose mission is to connect communities in Minnesota with U of MN faculty and students to advance community resilience through collaborative, course-based projects. RCP is a program of the Center for Urban and Regional Affairs (CURA). More information at http://www.rcp.umn.edu

SAKE (Single-cell RNA-Seq Analysis and Klustering Evaluation) Identifies Markers of Resistance to Targeted BRAF Inhibitors in Melanoma Cell Populations

Single-cell RNA-Seq’s (scRNA-Seq) unprecedented cellular resolution at a genome wide scale enables us to address questions about cellular heterogeneity that are inaccessible using methods that average over bulk tissue extracts. However, scRNA-Seq datasets also present additional challenges such as high transcript dropout rates, stochastic transcription events, and complex population substructures. Here, we present SAKE (Single-cell RNA-Seq Analysis and Klustering Evaluation): a robust method for scRNA-Seq analysis that provides quantitative statistical metrics at each step of the scRNA-Seq analysis pipeline including metrics for: the determination of the number of clusters present, the likelihood that each cell belongs to a given cluster, and the association of each gene marker in determining cluster membership. Comparing SAKE to multiple single-cell analysis methods shows that most methods perform similarly across a wide range cellular contexts, with SAKE outperforming these methods in the case of large complex populations. We next applied the SAKE algorithms to identify drug-resistant cellular populations as human melanoma cells respond to targeted BRAF inhibitors. Single-cell RNA-Seq data from both the Fluidigm C1 and 10x Genomics platforms were analyzed with SAKE to dissect this problem at multiple scales. Data from both platforms indicate that BRAF inhibitor resistant cells can emerge from rare populations already present before drug application, with SAKE identifying both novel and known markers of resistance. In addition, we compare integrated genomic and transcriptomic markers to show that resistance can arise stochastically within multiple distinct clonal populations

The Chemical Distribution in a Subluminous Type Ia Supernova: HST Images of the SN 1885 Remnant

We present HST images of the remnant of SN 1885 seen in absorption against M31's bulge via resonance lines of Ca I, Ca II, Fe I, and Fe II. Viewed in CaII H & K line absorption, the remnant appears as a nearly black circular spot with an outermost angular radius of 0.40" +/- 0.025" implying r = 1.52 pc and a 120 yr average expansion velocity of 12400 +/-1400 km/s. The strongest Ca II absorption is organized in a broken ring structure with a radius of 0.20" (=6000 km/s) with several apparent absorption `clumps' of an angular size near the pixel scale of 0.05" (= 1500 km/s). The detection of Ca II clumps is the first direct evidence for some instabilities and the existence of a deflagration phase in SNe Ia or, alternatively, mixing induced by radioactive decay of 56^Ni over time scales of seconds or days. However, the degree of mixing allowed by the observed images is much smaller than current 3D calculations for Rayleigh-Taylor dominated deflagration fronts. The images also require a central region of no or little Ca but iron group elements indicative of burning under sufficiently high densities for electron capture taking place, i.e., burning prior to a significant pre-expansion of the WD.Comment: 18 pages, 14 figures. Accepted for publication in Ap

The expansion asymmetry and age of the Cassiopeia A supernova remnant

HST images of the young supernova remnant Cas A are used to explore the expansion and spatial distribution of its highest velocity debris. ACS WFC images taken in 2004 March and December with Sloan F625W, F775W, and F850LP filters were used to identify 1825 high-velocity, outlying ejecta knots through measured proper motions of 0."35 - 0."90 yr(-1), corresponding to V-trans = 5500-14,500 km s(-1) assuming d = 3.4 kpc. The distribution of derived transverse expansion velocities for these ejecta knots shows a striking bipolar asymmetry with the highest velocity knots (V-trans >= 10,500 km s(-1)) confined to nearly opposing northeast and southwest "jets'' at P.A. = 45 degrees-70 degrees and 230 degrees-270 degrees, respectively. The jets have about the same maximum expansion velocity of similar or equal to 14,000 km s(-1) and appear kinematically and chemically distinct in that they are the remnant's only S-rich ejecta with expansion velocities above the 10,000-11,000 km s(-1) exhibited by outer nitrogen-rich ejecta, which otherwise represent the remnant's highest velocity debris. In addition, we find significant gaps in the spatial distribution of outlying ejecta in directions that are approximately perpendicular to the jets (P.A. = 145 degrees-200 degrees and 335 degrees-350 degrees). The remnant's central X-ray point source lies some 700 to the southeast of the estimated expansion center ( P.A. = 169 degrees +/- 8.degrees 4) indicating a projected motion toward the middle of the broad southern ejecta knot gap. Extrapolations of measured 9 month proper motions for all 1825 outer ejecta knots and a selected subsample of 72 bright and compact knots suggest explosion dates (assuming no knot deceleration) of 1662 +/- 27 and 1672 +/- 18, respectively. We find some evidence for nonuniform deceleration in different directions around the remnant and find 126 knots located along the northwestern limb among the least decelerated ejecta, suggesting a convergence date of 1681 +/- 19. A remnant age of around 325 yr would imply a +/- 350 km s(-1) transverse velocity for the central X-ray point source

LATE-TIME X-RAY, UV, AND OPTICAL MONITORING OF SUPERNOVA 1979C

ABSTRACT We present results from observations of supernova (SN) 1979C with the Newton X-Ray Multi-Mirror (XMMNewton) mission in X-rays and in UV, archival X-ray, and Hubble Space Telescope (HST ) data, and follow-up ground-based optical imaging. The XMM-Newton MOS spectrum shows the best-fit two-temperature thermal plasma emission characteristics of both the forward (kT high ¼ 4:1 þ76 À2:4 keV) and reverse shock (kT low ¼ 0:79 þ0:24 À0:17 keV) with no intrinsic absorption. The long-term X-ray light curve, constructed from all X-ray data available, reveals that SN 1979C is still radiating at a flux level similar to that detected by ROSAT in 1995, showing no sign of a decline over the last 6 years, some 16-23 yr after its outburst. The high inferred X-ray luminosity (L 0:3 2 ¼ 8 ; 10 38 ergs s À1 ) is caused by the interaction of the SN shock with dense circumstellar matter, likely deposited by a strong stellar wind from the progenitor with a high mass-loss rate ofṀ % 1:5 ; 10 À4 M yr À1 (v w /10 km s À1 ). The X-ray data support a strongly decelerated shock and show a mass-loss rate history that is consistent with a constant progenitor mass-loss rate and wind velocity over the past k16,000 yr in the stellar evolution of the progenitor. We find a best-fit circumstellar medium (CSM) density profile of CSM / r Às with index s P 1:7 and high CSM densities (k10 4 cm À

Tricks and treats: designing technology to support mobility assistance dogs

Assistance dogs are a key intervention to support the autonomy of people with tetraplegia. Previous research on assistive technologies have investigated ways to, ultimately, replace their labour using technology, for instance through the design of smart home environments. However, both the disability studies literature and our interviews suggest there is an immediate need to support these relationships, both in terms of training and bonding. Through a case study of an accessible dog treats dispenser, we investigate a technological intervention responding to these needs, detailing an appropriate design methodology and contributing insights into user requirements and preferences