Discussing “eScience and the Evolution of Library Services”

Following an eScience day at the University of Utah held on February 20, 2012, the National Network of Libraries of Medicine, MidContinental Region invited participants who attended either in person or via the broadcast to engage in an online discussion. This discussion provided the opportunity for them to debrief, continue to learn from each other, and share what was significant to them about the day. Using the research cycle as the focus, participants identified roles librarians could play, the skills and knowledge they needed, and the steps they should take in order to effectively support eScience. This article summarizes the ideas that resulted from their discussion

Multi-Institutional Collaborative Grants: Managing Institutional and Geographic Diversity

Objective: To present a model for developing, managing, and implementing multi-institutional collaborative funding that addresses challenges related to geographic and institutional diversity. Methods: Program Evaluation. This poster will address the topic of multi-institutional collaboration through a study of Tribal Connections Four Corners, an NN/LM subcontract funded project that is being carried out by seven institutions in six states. A retrospective analysis will be used to review the methods for developing, managing, and implementing the project. The findings of an external consulting group, which will be examining the collaborative aspect of the project, will be incorporated when relevant. Results: The program evaluation will result in the identification of best practices and lessons learned while developing, managing, and implementing the multi-institutional collaborative grant. Conclusions: Collaborative funding provides an opportunity to distribute the benefits of a project to a wider audience. This, combined with the increased focus on inter-organizational collaboration by the National Institutes of Health, illustrates the need for developing and sharing various models for managing multi-institutional projects. This poster will present one model for addressing the challenges that develop from the institutional and geographic diversity inherent in inter-organizational collaboration. As a result, those involved in or seeking multi-institutional funding will be better prepared to manage the collaborative project from its development through its implementation.https://digitalrepository.unm.edu/hslic-posters-presentations/1073/thumbnail.jp

Higher Sensitivity in Induction of Apoptosis in Fibroblast Cell Lines Derived from LEC Strain Rat to Ultraviolet B Radiation

LECラット由来繊維芽細胞のUVBに対する感受性をコロニー形成法を用いて調べた結果、LECラット細胞はWKAHラット細胞に比べてUVBに対して高い感受性を示した。D37値からLECラット細胞ではWKAH細胞の1.5倍以上高い感受性が認められた。ラジカルスキャベンジャーとして働く0.5MDMSOの存在下で細胞にUVBを照射するとLEC、WKAH細胞ともにUVB照射のみと比べて生存率に大きな違いは認められなかった。この結果からUVB照射によって生じるフリーラジカルはUVB誘発細胞死にはあまり関与しないと考えられる。LECラット細胞においてUVB照射後のアポトーシスをフローサイトメーターで検出したところ、照射線量に依存して増加した。一方、WKAHラット細胞ではUVB照射後アポトーシスの増加は認められなかった。これらの結果からLECラット細胞のUVBに対する高い感受性はUVB誘発アポトーシスに依拠していることが示唆された

Sub-Lethal Irradiation of Human Colorectal Tumor Cells Imparts Enhanced and Sustained Susceptibility to Multiple Death Receptor Signaling Pathways

Background: Death receptors (DR) of the TNF family function as anti-tumor immune effector molecules. Tumor cells, however, often exhibit DR-signaling resistance. Previous studies indicate that radiation can modify gene expression within tumor cells and increase tumor cell sensitivity to immune attack. The aim of this study is to investigate the synergistic effect of sub-lethal doses of ionizing radiation in sensitizing colorectal carcinoma cells to death receptor-mediated apoptosis. Methodology/Principal Findings: The ability of radiation to modulate the expression of multiple death receptors (Fas/ CD95, TRAILR1/DR4, TRAILR2/DR5, TNF-R1 and LTbR) was examined in colorectal tumor cells. The functional significance of sub-lethal doses of radiation in enhancing tumor cell susceptibility to DR-induced apoptosis was determined by in vitro functional sensitivity assays. The longevity of these changes and the underlying molecular mechanism of irradiation in sensitizing diverse colorectal carcinoma cells to death receptor-mediated apoptosis were also examined. We found that radiation increased surface expression of Fas, DR4 and DR5 but not LTbR or TNF-R1 in these cells. Increased expression of DRs was observed 2 days post-irradiation and remained elevated 7-days post irradiation. Sub-lethal tumor cell irradiation alone exhibited minimal cell death, but effectively sensitized three of three colorectal carcinoma cells to both TRAIL and Fasinduced apoptosis, but not LTbR-induced death. Furthermore, radiation-enhanced Fas and TRAIL-induced cell death lasted as long as 5-days post-irradiation. Specific analysis of intracellular sensitizers to apoptosis indicated that while radiation di

Commentary on J. Otto Lottes Health Sciences Library contributions to the National Network of Libraries of Medicine

The Associate Director of the National Network of Libraries of Medicine Mid-Continental Region, Claire Hamasu, offers congratulations to the J. Otto Lottes Health Sciences Library and provides commentary on the library's contributions to the NNLM on the occasion of its anniversary