978 research outputs found
Three-body monopole corrections to the realistic interactions
It is shown that a very simple three-body monopole term can solve practically
all the spectroscopic problems--in the , and shells--that were
hitherto assumed to need drastic revisions of the realistic potentials.Comment: 4 pages, 5figure
Multivalent Recognition of Peptides by Modular Self-Assembled Receptors
Developing nontraditional approaches to the synthesis and characterization of multivalent compounds is critical to our efforts to study and interface with biological systems and to build new noncovalent materials. This paper demonstrates a biomimetic approach to the construction of discrete, modular, multivalent receptors via molecular self-assembly in aqueous solution. Scaffolds presenting 1−3 viologen groups recruit a respective 1−3 copies of the synthetic host, cucurbit[8]uril, in a noncooperative manner and with a consistent equilibrium association constant (Ka) value of 2 × 106 M−1 per binding site. The assembled mono-, di-, and trivalent receptors bind to their cognate target peptides containing 1−3 Trp residues with Ka values in the range 1.7 × 104−4.7 × 106 M−1 and in predetermined mono- or multivalent binding modes with 31−280-fold enhancements in affinity and additive enthalpies due to multivalency. The extent of valency was determined directly by measuring the visible charge-transfer absorptivity due to the viologen−indole pair. The predictable behavior of this system and its ease of synthesis and analysis make it well suited to serve as a model for multivalent binding and for the multivalent recognition of peptides by design
Groundwater surface mapping informs sources of catchment baseflow
This work is funded by the Australian Research Council Discovery Project scheme through project DP120100253. We greatly appreciate the provision of groundwater chemistry data and introduction to the Gellibrand catchment by Alex Atkinson and Ian Cartwright from Monash University. We thank two anonymous reviewers and Ian Cartwright for their insightful and constructive reviews that helped improve this paper.Peer reviewedPublisher PD
Life table analysis of Diaphorina citri (Hemiptera: Psyllidae) infesting sweet orange (Citrus sinensis) in São Paulo
An ecological life table for eggs and nymphs of Diaphorina citri Kuwayama (Hemiptera:
Psyllidae) was constructed with data obtained from orange orchards (Citrus sinensis Osbeck) in 2 regions of the State of São Paulo, over 4 generations in the period from XI-2006
to V-2007, comprising spring, summer, and fall seasons. Young growing shoots with D. citri
eggs present were identifed, and live individuals were counted until adult emergence. No
predatory arthropods were observed in association with D. citri eggs and nymphs during
the study. The mean parasitism of fourth- and ffth-instar nymphs by Tamarixia radiata
Waterston (Hymenoptera: Eulophidae) was 2.3%. The durations of the egg–adult period
were similar among the 4 generations, ranging from 18.0 to 24.7 d (at mean temperatures
ranging from 21.6 to 26.0 °C) and followed the temperature requirement models obtained
in the laboratory for D. citri. However, survival from the egg to the adult stage for the same
period varied considerably from 1.7 to 21.4%; the highest mortalities were observed in the
egg and small nymphal (frst- to thirdinstar) stages, which were considered to be key phases
for population growth of the pest.Uma tabela de vida ecológica foi construída para ovos e ninfas de Diaphorina citri Kuwayama (Hemiptera: Psyllidae) com dados obtidos em pomares de laranja (Citrus sinensis Osbeck) em 2 regiões do estado de São Paulo, com 4 gerações, no período de novembro de 2006
a maio de 2007, compreendendo as estações de primavera, verão e outono. Ramos jovens
em crescimento com a presença de ovos de D. citri foram identificados e os indivíduos vivos
foram contados até a emergência dos adultos. Nenhum predador foi observado associado a
ovos e ninfas de D. citri durante o estudo. A taxa média de parasitismo de ninfas de quarto
e quinto ínstares por Tamarixia radiata Waterson (Hymenoptera: Eulophidae) foi de 2.3%.
A duração do período de ovo a adulto foi semelhante entre as quatro gerações, variando de
18.0 a 24.7 dias (com temperaturas médias de 21.6 a 26.0 °C) e seguiram os modelos de
exigencias térmicas obtidas em laboratório para D. citri. Todavia, a sobrevivencia de ovo
até o estágio adulto variou consideravelmente para o mesmo período, de 1.7 a 21.4%, sendo
que as maiores mortalidades foram observadas nos estágios de ovos e ninfas pequenas (de
primeiro a terceiro ínstares), as quais foram consideradas fases chaves para o crescimento
populacional desta praga.info:eu-repo/semantics/publishedVersio
A phase II trial of bryostatin 1 in patients with non-Hodgkin's lymphoma
Bryostatin 1 is a naturally occurring macrocyclic lactone with promising antitumour and immunomodulatory function in preclinical and phase I clinical investigations. In this phase II study, 17 patients with progressive non-Hodgkin's lymphoma of indolent type (NHL), previously treated with chemotherapy, received a median of 6 (range 1–9) intravenous infusions of 25 μg/m2bryostatin 1 given once weekly over 24 hours. In 14 evaluable patients no responses were seen. Stable disease was attained in one patient for 9 months. The principal toxicities were myalgia and phlebitis. Treatment was discontinued early because of toxicity alone (phlebitis) in 2 patients, toxicity in addition to progressive disease in 3 patients (myalgia and phlebitis n = 2; thrombocytopenia n = 1) and progressive disease in 5 patients. The results fail to demonstrate efficacy of this regimen of bryostatin 1 in the treatment of NHL. In light of preclinical data that demonstrate synergy between bryostatin 1 and several cytotoxic agents and cytokines, clinical studies to investigate bryostatin 1 in combination are warranted. We also present data to demonstrate that central venous lines may be used in future studies to avoid phlebitis. © 2001 Cancer Research Campaign http://www.bjcancer.co
The Birmingham Boron Neutron Capture Therapy (BNCT) project : developments towards selective internal particle therapy
This paper will review progress on two aspects of the Birmingham BNCT project. Firstly on evaluation of the effects of high and low LET radiations when delivered simultaneously, and secondly on attempts to optimise delivery of the boron carrier compound BPA through pharmacokinetic studies. Simultaneous or non-simultaneous irradiations of V79 cells with alpha-particle and X-ray irradiations were performed. Alpha doses of 2 and 2.5 Gy were chosen and the impact on survival when delivered separately or simultaneously with variable doses of X-rays was evaluated. The pharmacokinetics of the delivery of a new formulation of BPA (BPA-mannitol) are being investigated in brain tumour patients through a study with 2 × 2 design featuring intravenous and intracarotid artery infusion of BPA, with or without a mannitol bolus. On the combined effect of low and high LET radiations, a synergistic effect was observed when alpha and X-ray doses are delivered simultaneously. The effect is only present at the 2.5 Gy alpha dose and is a very substantial effect on both the shape of the survival curve and the level of cell killing. This indicates that the alpha component may have the effect of inhibiting the repair of damage from the low LET radiation dose delivered simultaneously. On the pharmacokinetics of BPA, data on the first three cohorts indicate that bioavailability of BPA in brain ECF is increased substantially through the addition of a mannitol bolus, as well as by the use of intracarotid artery route of infusion. In both cases, for some patients the levels after infusion approach those seen in blood, whereas the ECF levels for intravenous infusion without mannitol are typically less than 10% of the blood values
The validity of using ICD-9 codes and pharmacy records to identify patients with chronic obstructive pulmonary disease
Background: Administrative data is often used to identify patients with chronic obstructive pulmonary disease (COPD), yet the validity of this approach is unclear. We sought to develop a predictive model utilizing administrative data to accurately identify patients with COPD.
Methods: Sequential logistic regression models were constructed using 9573 patients with postbronchodilator spirometry at two Veterans Affairs medical centers (2003-2007). COPD was defined as: 1) FEV1/FVC <0.70, and 2) FEV1/FVC < lower limits of normal. Model inputs included age, outpatient or inpatient COPD-related ICD-9 codes, and the number of metered does inhalers (MDI) prescribed over the one year prior to and one year post spirometry. Model performance was assessed using standard criteria.
Results: 4564 of 9573 patients (47.7%) had an FEV1/FVC < 0.70. The presence of ≥1 outpatient COPD visit had a sensitivity of 76% and specificity of 67%; the AUC was 0.75 (95% CI 0.74-0.76). Adding the use of albuterol MDI increased the AUC of this model to 0.76 (95% CI 0.75-0.77) while the addition of ipratropium bromide MDI increased the AUC to 0.77 (95% CI 0.76-0.78). The best performing model included: ≥6 albuterol MDI, ≥3 ipratropium MDI, ≥1 outpatient ICD-9 code, ≥1 inpatient ICD-9 code, and age, achieving an AUC of 0.79 (95% CI 0.78-0.80).
Conclusion: Commonly used definitions of COPD in observational studies misclassify the majority of patients as having COPD. Using multiple diagnostic codes in combination with pharmacy data improves the ability to accurately identify patients with COPD.Department of Veterans Affairs, Health Services Research and Development (DHA), American Lung Association (CI- 51755-N) awarded to DHA, the American Thoracic Society Fellow Career Development AwardPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/84155/1/Cooke - ICD9 validity in COPD.pd
Use of terahertz-Raman spectroscopy to determine solubility of the crystalline active pharmaceutical ingredient in polymeric matrices during hot melt extrusion
Polymer-based amorphous solid dispersions (ASDs) comprise one of the most promising formulation strategies devised to improve the oral bioavailability of poorly water-soluble drugs. Exploitation of such systems in marketed products has been limited because of poor understanding of physical stability. The internal disordered structure and increased free energy provide a thermodynamic driving force for phase separation and recrystallization, which can compromise therapeutic efficacy and limit product shelf life. A primary concern in the development of stable ASDs is the solubility of the drug in the polymeric carrier, but there is a scarcity of reliable analytical techniques for its determination. In this work, terahertz (THz) Raman spectroscopy was introduced as a novel empirical approach to determine the saturated solubility of crystalline active pharmaceutical ingredient (API) in polymeric matrices directly during hot melt extrusion. The solubility of a model compound, paracetamol, in two polymer systems, Affinisol 15LV (HPMC) and Plasdone S630 (copovidone), was determined by monitoring the API structural phase transitions from crystalline to amorphous as an excess of crystalline drug dissolved in the polymeric matrix. THz-Raman results enabled construction of solubility phase diagrams and highlighted significant differences in the solubilization capacity of the two polymer systems. The maximum stable API-load was 20 wt % for Affinisol 15LV and 40 wt % for Plasdone S630. Differential scanning calorimetry and XRPD studies corroborated these results. This approach has demonstrated a novel capability to provide real-time API-polymer phase equilibria data in a manufacturing relevant environment and promising potential to predict solid-state solubility and physical stability of ASDs
Collective Flow from the Intranuclear Cascade Model
The phenomenon of collective flow in relativistic heavy ion collisions is
studied using the hadronic cascade model ARC. Direct comparison is made to data
gathered at the Bevalac, for Au+Au at GeV/c. In contrast to the
standard lore about the cascade model, collective flow is well described
quantitatively without the need for explicit mean field terms to simulate the
nuclear equation of state. Pion collective flow is in the opposite direction to
nucleon flow as is that of anti-nucleons and other produced particles. Pion and
nucleon flow are predicted at AGS energies also, where, in light of the higher
baryon densities achieved, we speculate that equation of state effects may be
observable.Comment: 9 pages, 2 figures include
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