Dramatic Increases of Soil Microbial Functional Gene Diversity at the Treeline Ecotone of Changbai Mountain.

The elevational and latitudinal diversity patterns of microbial taxa have attracted great attention in the past decade. Recently, the distribution of functional attributes has been in the spotlight. Here, we report a study profiling soil microbial communities along an elevation gradient (500-2200 m) on Changbai Mountain. Using a comprehensive functional gene microarray (GeoChip 5.0), we found that microbial functional gene richness exhibited a dramatic increase at the treeline ecotone, but the bacterial taxonomic and phylogenetic diversity based on 16S rRNA gene sequencing did not exhibit such a similar trend. However, the β-diversity (compositional dissimilarity among sites) pattern for both bacterial taxa and functional genes was similar, showing significant elevational distance-decay patterns which presented increased dissimilarity with elevation. The bacterial taxonomic diversity/structure was strongly influenced by soil pH, while the functional gene diversity/structure was significantly correlated with soil dissolved organic carbon (DOC). This finding highlights that soil DOC may be a good predictor in determining the elevational distribution of microbial functional genes. The finding of significant shifts in functional gene diversity at the treeline ecotone could also provide valuable information for predicting the responses of microbial functions to climate change

Message Feedback Interference Cancellation Aided UAMP Iterative Detector for OTFS Systems

The designing of efficient signal detectors is important and yet challenge for orthogonal time frequency space (OTFS) systems in high-mobility scenarios. In this letter, we develop an efficient message feedback interference cancellation aided unitary approximate message passing (denoted as UAMPMFIC) iterative detector, where the latest feedback messages from variable nodes are utilized for more reliable interference cancellation and performance improvement. A fast recursive scheme is leveraged in the proposed UAMP-MFIC detector to prevent complexity increasing. To further alleviate the error-propagation and improve the receiver performance, we also develop the bidirectional symbol detection structures, where Turbo UAMP-MFIC detector and iterative weight UAMP-MFIC detector are proposed to efficiently fuse the estimation results of forward and backward UAMP-MFIC detectors. The simulation results are finally provided to demonstrate performance improvement of our proposed detectors over existing detectors

Delay-Doppler Reversal for OTFS System in Doubly-selective Fading Channels

The recent proposed orthogonal time frequency space (OTFS) modulation shows signifcant advantages than conventional orthogonal frequency division multiplexing (OFDM) for high mobility wireless communications. However, a challenging problem is the development of effcient receivers for practical OTFS systems with low complexity. In this paper, we propose a novel delay-Doppler reversal (DDR) technology for OTFS system with desired performance and low complexity. We present the DDR technology from a perspective of two-dimensional cascaded channel model, analyze its computational complexity and also analyze its performance gain compared to the direct processing (DP) receiver without DDR. Simulation results demonstrate that our proposed DDR receiver outperforms traditional receivers in doubly-selective fading channels

The Economic Impact of Film Tourism: The Case of the Lord of the Rings and Hobbit

Film tourism, also known as popular culture tourism, is tourism in the wake of film shows. Research literature contains no established and accepted method for measuring the economic effects of film tourism. This paper takes the first step to evaluate the overall economic impacts of film tourism, with a particular focus on the Lord of the Rings and the Hobbit filmed in New Zealand. A new approach that combines both econometric and computable general equilibrium modeling techniques is used in the impact assessment. The preliminary results show that the Lord of the Rings did not bring any significant impacts on the tourism and economy of New Zealand mainly due to its lack of appropriate marketing strategies. The Hobbit film, on the other hand, was found to have brought significant positive impacts on the New Zealand economy through film induced tourism

Structure of the NS1 Protein N-Terminal Origin Recognition/Nickase Domain from the Emerging Human Bocavirus

This is the publisher's version, also available electronically from http://www.asm.org/Human bocavirus is a newly identified, globally prevalent, parvovirus that is associated with respiratory infection in infants and young children. Parvoviruses encode a large nonstructural protein 1 (NS1) that is essential for replication of the viral single-stranded DNA genome and DNA packaging and may play versatile roles in virus-host interactions. Here, we report the structure of the human bocavirus NS1 N-terminal domain, the first for any autonomous parvovirus. The structure shows an overall fold that is canonical to the histidine-hydrophobic-histidine superfamily of nucleases, which integrates two distinct DNA-binding sites: (i) a positively charged region mediated by a surface hairpin (residues 190 to 198) that is responsible for recognition of the viral origin of replication of the double-stranded DNA nature and (ii) the nickase active site that binds to the single-stranded DNA substrate for site-specific cleavage. The structure reveals an acidic-residue-rich subdomain that is present in bocavirus NS1 proteins but not in the NS1 orthologs in erythrovirus or dependovirus, which may mediate bocavirus-specific interaction with DNA or potential host factors. These results provide insights into recognition of the origin of replication and nicking of DNA during bocavirus genome replication. Mapping of variable amino acid residues of NS1s from four human bocavirus species onto the structure shows a scattered pattern, but the origin recognition site and the nuclease active site are invariable, suggesting potential targets for antivirals against this clade of highly diverse human viruses

Proximate grassland and shrub-encroached sites show dramatic restructuring of soil bacterial communities.

Background: Changes in aboveground community composition and diversity following shrub encroachment have been studied extensively. Recently, shrub encroachment was associated with differences in belowground bacterial communities relative to non-encroached grassland sites hundreds of meters away. This spatial distance between grassland and shrub sites left open the question of how soil bacterial communities associated with different vegetation types might differ within the same plot location. Methods: We examined soil bacterial communities between shrub-encroached and adjacent (one m apart) grassland soils in Chinese Inner Mongolian, using high-throughput sequencing method (Illumina, San Diego, CA, USA). Results: Shrub-encroached sites were associated with dramatic restructuring of soil bacterial community composition and predicted metabolic function, with significant increase in bacterial alpha-diversity. Moreover, bacterial phylogenic structures showed clustering in both shrub-encroached and grassland soils, suggesting that each vegetation type was associated with a unique and defined bacterial community by niche filtering. Finally, soil organic carbon (SOC) was the primary driver varied with shifts in soil bacterial community composition. The encroachment was associated with elevated SOC, suggesting that shrub-mediated shifts in SOC might be responsible for changes in belowground bacterial community. Discussion: This study demonstrated that shrub-encroached soils were associated with dramatic restructuring of bacterial communities, suggesting that belowground bacterial communities appear to be sensitive indicators of vegetation type. Our study indicates that the increased shrub-encroached intensity in Inner Mongolia will likely trigger large-scale disruptions in both aboveground plant and belowground bacterial communities across the region

Towards Understanding the Causal Relationships in Proliferating SD Education—A System Dynamics Group Modelling Approach in China

Given the growing importance of system dynamics (SD) in solving increasingly complex and dynamic problems in any country, we believe SD education will become an imperative leverage point in helping us deal with our uncertain future. This study tries to understand the causal relationships in proliferating SD education by a system dynamics group modelling approach in China. Based on a questionnaire survey and a group model building (GMB) workshop, we aim to explore the interactions of feedback loops in the constructed causal loop diagram (CLD). This uncovers insights into what constitutes the growth of SD education in China and helps to guide the design and implementation of policies to achieve this growth. We conclude that it is important and relevant to find ways to improve, including the construction of an SD teaching platform to integrate normative resources, providing opportunities for teacher training, enhancing the availability and accessibility of SD education, and building networks with international partners. The results of our study may set the foundation for further research to extend the generalizability of our insights and methodological approaches to other countries.publishedVersio