3D modelling Yar City : application of UAVs for archaeological research

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Defining Key Genes Regulating Morphogenesis of Apocrine Sweat Gland in Sheepskin

The apocrine sweat gland is a unique skin appendage in humans compared to mouse and chicken models. The absence of apocrine sweat glands in chicken and murine skin largely restrains further understanding of the complexity of human skin biology and skin diseases, like hircismus. Sheep may serve as an additional system for skin appendage investigation owing to the distributions and histological similarities between the apocrine sweat glands of sheep trunk skin and human armpit skin. To understand the molecular mechanisms underlying morphogenesis of apocrine sweat glands in sheepskin, transcriptome analyses were conducted to reveal 1631 differentially expressed genes that were mainly enriched in three functional groups (cellular component, molecular function and biological process), particularly in gland, epithelial, hair follicle and skin development. There were 7 Gene Ontology (GO) terms enriched in epithelial cell migration and morphogenesis of branching epithelium that were potentially correlated with the wool follicle peg elongation. An additional 5 GO terms were enriched in gland morphogenesis (20 genes), gland development (42 genes), salivary gland morphogenesis and development (8 genes), branching involved in salivary gland morphogenesis (6 genes) and mammary gland epithelial cell differentiation (4 genes). The enriched gland-related genes and two Kyoto Encyclopedia of Genes and Genomes pathway genes (WNT and TGF-β) were potentially involved in the induction of apocrine sweat glands. Genes named BMPR1A, BMP7, SMAD4, TGFB3, WIF1, and WNT10B were selected to validate transcript expression by qRT-PCR. Immunohistochemistry was performed to localize markers for hair follicle (SOX2), skin fibroblast (PDGFRB), stem cells (SOX9) and BMP signaling (SMAD5) in sheepskin. SOX2 and PDGFRB were absent in apocrine sweat glands. SOX9 and SMAD5 were both observed in precursor cells of apocrine sweat glands and later in gland ducts. These results combined with the upregulation of BMP signaling genes indicate that apocrine sweat glands were originated from outer root sheath of primary wool follicle and positively regulated by BMP signaling. This report established the primary network regulating early development of apocrine sweat glands in sheepskin and will facilitate the further understanding of histology and pathology of apocrine sweat glands in human and companion animal skin

Hollow mesoporous silica nanoparticles for intracellular delivery of fluorescent dye

In this study, hollow mesoporous silica nanoparticles (HMSNs) were synthesized using the sol-gel/emulsion approach and its potential application in drug delivery was assessed. The HMSNs were characterized, by transmission electron microscopy (TEM), Scanning Electron Microscopy (SEM), nitrogen adsorption/desorption and Brunauer-Emmett-Teller (BET), to have a mesoporous layer on its surface, with an average pore diameter of about 2 nm and a surface area of 880 m2/g. Fluorescein isothiocyanate (FITC) loaded into these HMSNs was used as a model platform to assess its efficacy as a drug delivery tool. Its release kinetic study revealed a sequential release of FITC from the HMSNs for over a period of one week when soaked in inorganic solution, while a burst release kinetic of the dye was observed just within a few hours of soaking in organic solution. These FITC-loaded HMSNs was also found capable to be internalized by live human cervical cancer cells (HeLa), wherein it was quickly released into the cytoplasm within a short period of time after intracellular uptake. We envision that these HMSNs, with large pores and high efficacy to adsorb chemicals such as the fluorescent dye FITC, could serve as a delivery vehicle for controlled release of chemicals administered into live cells, opening potential to a diverse range of applications including drug storage and release as well as metabolic manipulation of cells

Insight-HXMT observations of Swift J0243.6+6124 during its 2017-2018 outburst

The recently discovered neutron star transient Swift J0243.6+6124 has been monitored by {\it the Hard X-ray Modulation Telescope} ({\it Insight-\rm HXMT). Based on the obtained data, we investigate the broadband spectrum of the source throughout the outburst. We estimate the broadband flux of the source and search for possible cyclotron line in the broadband spectrum. No evidence of line-like features is, however, found up to $\rm 150~keV$. In the absence of any cyclotron line in its energy spectrum, we estimate the magnetic field of the source based on the observed spin evolution of the neutron star by applying two accretion torque models. In both cases, we get consistent results with $B\rm \sim 10^{13}~G$, $D\rm \sim 6~kpc$ and peak luminosity of $\rm >10^{39}~erg~s^{-1}$ which makes the source the first Galactic ultraluminous X-ray source hosting a neutron star.Comment: publishe

Overview to the Hard X-ray Modulation Telescope (Insight-HXMT) Satellite

As China's first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band (1-250 keV) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 MeV. It was designed to perform pointing, scanning and gamma-ray burst (GRB) observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed. Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results.Comment: 29 pages, 40 figures, 6 tables, to appear in Sci. China-Phys. Mech. Astron. arXiv admin note: text overlap with arXiv:1910.0443

Finite-Time Control for a Coupled Four-Tank Liquid Level System Based on the Port-Controlled Hamiltonian Method

This work investigates the finite-time control problem for a nonlinear four-tank cross-coupled liquid level system by the port-controlled Hamiltonian (PCH) model. A fixed-free methodology is exhibited which can be used to simplify the controller design procedure. To get an adjustable convergent gain of the finite-time control, a feasible technique named damping normalization is proposed. A novel parameter autotuning algorithm is given to clarify the principle of choosing parameters of the PCH method. Furthermore, a finite-time controller is designed by a state-error desired Hamiltonian function, and the relationship between the settling time and a parameter is given, which can be applied in practical engineering easily to adjust the settling time according to the industrial need. Finally, simulation and experimental results verify the effectiveness of the proposed algorithm

Study on the flotation technology and adsorption mechanism of galena–jamesonite separation

In order to further separate the concentrate containing galena and jamesonite before undergoing hydrometallurgical process, flotation experiment was performed on the basis of mineralogical analysis. And the adsorption mechanisms of collector H on galena and jamesonite were also studied by FT-IR spectra analysis and molecular dynamics (MD) simulation. The flotation result shows that the efficient separation can be achieved with H as selective collector. Galena concentrated with Pb grade of 72.09% and Pb recovery of 50.96% was obtained, and jamesonite concentrated with Sb grade and recovery of 10.89% and 76.67% respectively was obtained as well. Infrared spectrum analysis indicates that collector H can adsorb on the surface of galena and react with Pb2+ to generate hydrophobic salt, while no evident adsorption phenomenon was observed on the surface of jamesonite. The MD simulation and calculation results demonstrate that adsorption energy of collector H on galena and jamesonite surface is −872.74 kJ/mol and −500.538 kJ/mol, respectively, which means collector H is easier to adsorb on the surface of galena than that of jamesonite. Keywords: Mixed concentration, Galena, Jamesonite, Flotation, Molecular dynamic simulation, Adsorption energ

Disturbance Observer and L2-Gain-Based State Error Feedback Linearization Control for the Quadruple-Tank Liquid-Level System

This paper proposes a fresh state error feedback linearization control method with disturbance observer (DOB) and L2 gain for a quadruple-tank liquid-level system. Firstly, in terms of the highly nonlinear and strong coupling characteristics of the quadruple-tank system, a state error feedback linearization technique is employed to design the controller to achieve liquid-level position control and tracking control. Secondly, DOB is purposed to estimate uncertain exogenous disturbances and applied to compensation control. Moreover, an L2-gain disturbance attenuation technology is designed to resolve one class of disturbance problem by uncertain parameter perturbation existing in the quadruple-tank liquid-level system. Finally, compared with the classical proportion integration differentiation (PID) and sliding mode control (SMC) methods, the extensive experimental results validate that the proposed strategy has good position control, tracking control, and disturbance rejection performances