Monitoring activity at the Daguangbao mega-landslide (China) using Sentinel-1 TOPS time series interferometry

The Daguangbao mega-landslide (China), induced by the 2008 Wenchuan earthquake (Mw = 7.9), with an area of approximately 8 km2, is one of the largest landslides in the world. Experts predicted that the potential risk and instability of the landslide might remain for many decades, or even longer. Monitoring the activity of such a large landslide is hence critical. Terrain Observation by Progressive Scans (TOPS) mode from the Sentinel-1 satellite provides us with up-to-date high-quality Synthetic Aperture Radar (SAR) images over a wide ground coverage (250 × 250 km), enabling full exploitation of various InSAR applications. However, the TOPS mode introduces azimuth-dependent Doppler variations to radar signals, which requires an additional processing step especially for SAR interferometry. Sentinel-1 TOPS data have been widely applied to earthquakes, but the performance of TOPS data-based time series analysis requires further exploitation. In this study, Sentinel-1 TOPS data were employed to investigate landslide post-seismic activities for the first time. To deal with the azimuth-dependent Doppler variations, a processing chain of TOPS time series interferometry approach was developed. Since the Daguangbao landslide is as a result of the collapse of a whole mountain caused by the 2008 Mw 7.9 Wenchuan earthquake, the existing Digital Elevation Models (DEMs, e.g. SRTM and ASTER) exhibit height differences of up to approximately 500 m. Tandem-X images acquired after the earthquake were used to generate a high resolution post-seismic DEM. The high gradient topographic errors of the SRTM DEM (i.e. the differences between the pre-seismic SRTM and the actual post-seismic elevation), together with low coherence in mountainous areas make it difficult to derive a precise DEM using the traditional InSAR processing procedure. A re-flattening iterative method was hence developed to generate a precise TanDEM-X DEM in this study. The volume of the coseismic Daguangbao landslide was estimated to be of 1.189 ± 0.110 × 109 m3 by comparing the postseismic Tandem-X DEM with the preseismic SRTM DEM, which is consistent with the engineering geological survey result. The time-series results from Sentinel-1 show that some sectors of the Daguangbao landslide are still active (and displaying four sliding zones) and exhibiting a maximum displacement rate of 8 cm/year, even eight years after the Wenchuan earthquake. The good performance of TOPS in this time series analysis indicates that up-to-date high-quality TOPS data with spatiotemporal baselines offer significant potential in terms of future InSAR applications.This work was supported by the National Natural Science Foundation of China under Grant No. 41474003. The research stay of Dr. Tomás at Newcastle University was funded by the Ministry of Education, Culture and Sport within the framework of Project PRX14/00100. Additional funding was obtained from the Spanish Government under projects TIN2014-55413-C2-2-P and ESP2013-47780-C2-2-R. Part of this work is also supported by the UK Natural Environmental Research Council (NERC) through the Centre for the Observation and Modelling of Earthquakes, Volcanoes and Tectonics (COMET, ref.: come30001) and the LICS and CEDRRiC projects (ref. NE/K010794/1 and NE/N012151/1, respectively), the ESA-MOST DRAGON-3 projects (ref. 10607 and 10665), the ESA-MOST DRAGON-4 project (ref. 32244) and the Open Fund from the Key Laboratory of Earth Fissures Geological Disaster, Ministry of Land and Resources (ref.: gla2013001)

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

Essential transition metals and non-essential metals often co-exist in arable soils. In plants, some transition metal transporters, such as the natural resistance-associated macrophage proteins (NRAMPs), poorly selectively transport metals with similar chemical properties whether they are essential or non-essential. In this study, a member of the NRAMP transporter family, TpNRAMP3, was identified from dwarf Polish wheat (Triticum polonicum L.). TpNRAMP3 encodes a plasma membrane-localized protein and was highly expressed in leaf blades and roots at the jointing and booting stages, and in the first nodes at the grain filling stage. Expression of TpNRAMP3 increased sensitivity to Cd and Co, but not Zn, and increased the Cd and Co concentrations in yeast. TpNRAMP3 expression in Arabidopsis increased concentrations of Cd, Co, and Mn, but not Fe or Zn, in roots, shoots, and whole plant. However, TpNRAMP3 did not affect translocation of Cd, Co, or Mn from roots to shoots. These results suggest that TpNRAMP3 is a transporter for Cd, Co, and Mn accumulation, but not for Fe or Zn. However, Cd and Co are non-essential toxic metals; selective genetic manipulation of TpNRAMP3 will help breed low Cd- and Co-accumulating cultivars

Introgression of Chromosome 3Ns from Psathyrostachys huashanica into Wheat Specifying Resistance to Stripe Rust

Wheat stripe rust is a destructive disease in the cool and humid wheat-growing areas of the world. Finding diverse sources of stripe rust resistance is critical for increasing genetic diversity of resistance for wheat breeding programs. Stripe rust resistance was identified in the alien species Psathyrostachys huashanica, and a wheat- P. huashanica amphiploid line (PHW-SA) with stripe rust resistance was reported previously. In this study, a P. huashanica 3Ns monosomic addition line (PW11) with superior resistance to stripe rust was developed, which was derived from the cross between PHW-SA and wheat J-11. We evaluated the alien introgressions PW11-2, PW11-5 and PW11-8 which were derived from line PW11 for reaction to new Pst race CYR32, and used molecular and cytogenetic tools to characterize these lines. The introgressions were remarkably resistant to CYR32, suggesting that the resistance to stripe rust of the introgressions thus was controlled by gene(s) located on P. huashanica chromosome 3Ns. All derived lines were cytologically stable in term of meiotic chromosome behavior. Two 3Ns chromosomes of P. huashanica were detected in the disomic addition line PW11-2. Chromosomes 1B of substitution line PW11-5 had been replaced by a pair of P. huashanica 3Ns chromosomes. In PW11-8, a small terminal segment from P. huashanica chromosome arm 3NsS was translocated to the terminal region of wheat chromosomes 3BL. Thus, this translocated chromosome is designated T3BL-3NsS. These conclusions were further confirmed by SSR analyses. Two 3Ns-specific markers Xgwm181 and Xgwm161 will be useful to rapidly identify and trace the translocated fragments. These introgressions, which had significant characteristics of resistance to stripe rust, could be utilized as novel germplasms for wheat breeding

RNA-seq analysis revealed considerable genetic diversity and enabled the development of specific KASP markers for Psathyrostachys huashanica

Psathyrostachys huashanica, which grows exclusively in Huashan, China, is an important wild relative of common wheat that has many desirable traits relevant for wheat breeding. However, the poorly characterized interspecific phylogeny and genomic variations and the relative lack of species-specific molecular markers have limited the utility of P. huashanica as a genetic resource for enhancing wheat germplasm. In this study, we sequenced the P. huashanica transcriptome, resulting in 50,337,570 clean reads that were assembled into 65,617 unigenes, of which 38,428 (58.56%) matched at least one sequence in public databases. The phylogenetic analysis of P. huashanica, Triticeae species, and Poaceae species was conducted using 68 putative orthologous gene clusters. The data revealed the distant evolutionary relationship between P. huashanica and common wheat as well as the substantial diversity between the P. huashanica genome and the wheat D genome. By comparing the transcriptomes of P. huashanica and Chinese Spring, 750,759 candidate SNPs between P. huashanica Ns genes and their common wheat orthologs were identified. Among the 90 SNPs in the exon regions with different functional annotations, 58 (64.4%) were validated as Ns genome-specific SNPs in the common wheat background by KASP genotyping assays. Marker validation analyses indicated that six specific markers can discriminate between P. huashanica and the other wheat-related species. In addition, five markers are unique to P. huashanica, P. juncea, and Leymus species, which carry the Ns genome. The Ns genome-specific markers in a wheat background were also validated regarding their specificity and stability for detecting P. huashanica chromosomes in four wheat–P. huashanica addition lines. Four and eight SNP markers were detected in wheat–P. huashanica 2Ns and 7Ns addition lines, respectively, and one marker was specific to both wheat–P. huashanica 3Ns, 4Ns, and 7Ns addition lines. These markers developed using transcriptome data may be used to elucidate the genetic relationships among Psathyrostachys, Leymus, and other closely-related species. They may also facilitate precise introgressions and the high-throughput monitoring of P. huashanica exogenous chromosomes or segments in future crop breeding programs

Genetic mutations in ribosomal biogenesis gene TCOF1 identified in human neural tube defects

Abstract Background Rare mutations in multiple genes have been associated with human neural tube defects (NTDs), but their causative roles in NTDs disease are poorly understood. Insufficiency of the ribosomal biogenesis gene treacle ribosome biogenesis factor 1(Tcof1) results in cranial NTDs and craniofacial malformations in mice. Here, we aimed to identify genetic association of TCOF1 with human NTDs. Methods High‐throughput sequencing targeted on TCOF1 was performed on samples from 355 human cases affected by NTDs and 225 controls from a Han Chinese population. Results Four novel missense variants were found in the NTD cohort. Cell‐based assays indicated that the p.(A491G) variant carried by an individual, who shows anencephaly and single‐nostril abnormality, attenuates production of total proteins, suggesting a loss‐of‐function mutation in ribosomal biogenesis. Importantly, this variant promotes nucleolar disruption and stabilizes p53 protein, highlighting an unbalancing effect on cell apoptosis. Conclusions This study explored the functional impact of a missense variant in TCOF1, implicating a set of novel causative biological factors involved in the pathogenicity of human NTDs, particularly whom combined with craniofacial abnormality

Optimizing Energy Efficiency in Metro Systems Under Uncertainty Disturbances Using Reinforcement Learning

In the realm of urban transportation, metro systems serve as crucial and sustainable means of public transit. However, their substantial energy consumption poses a challenge to the goal of sustainability. Disturbances such as delays and passenger flow changes can further exacerbate this issue by negatively affecting energy efficiency in metro systems. To tackle this problem, we propose a policy-based reinforcement learning approach that reschedules the metro timetable and optimizes energy efficiency in metro systems under disturbances by adjusting the dwell time and cruise speed of trains. Our experiments conducted in a simulation environment demonstrate the superiority of our method over baseline methods, achieving a traction energy consumption reduction of up to 10.9% and an increase in regenerative braking energy utilization of up to 47.9%. This study provides an effective solution to the energy-saving problem of urban rail transit.Comment: 11 page