Individual Professional Practice in the Company

Import 23/08/2017Cílem této bakalářské práce je popsat absolvování odborné praxe ve firmě HS Interactive s.r.o. Praxe byla zaměřena na vývoj mobilní aplikace pro operační systém Android. Aplikace je mobilním klientem pro sociální síť MatchToMe. V úvodu popisuji důvody, které vedly k výběru odborné praxe. Dále se věnuji úkolům, které mi byly zadány s jejich implementací a postupem řešení problémů, které se objevily při vývoji. Závěr práce je věnován zhodnocení získaných zkušeností a dosažených výsledků.Purpose of this bachelor thesis is to describe a professional practice in company HS Interactive s.r.o. Practice was focused on the development of mobile application for the operating system Android. The application is a mobile client for social network MatchToMe. In the introduction I describe reasons that led to the selection of professional practice. Then I describe tasks that I have been awarded with their implementations and process of solution issues that have emerged during development. The conclusion of thesis is dedicated to the evaluation of the experience gained and the results achieved.440 - Katedra telekomunikační technikyvýborn

Suppressive effects of edaravone on ROS in primary HCEpiCs exposed to hyperosmotic media.

<p>ROS was evaluated via DCFH-DA staining (ANOVA: *, P<0.001 vs. untreated control; #, P<0.001 vs. 450 mOsM treated group).</p

Suppressive effects of edaravone on mitochondrial function at 10 or 20 μM.

<p>ROS in primary HCEpiCs exposed to hyperosmotic media (400–450 mOsM), evaluated via MitoSox Red staining (ANOVA: *, P<0.001 vs. untreated control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on cytosol cytochrome C release as measured by western blot analysis.

<p>Results indicate that edaravone treatment attenuates hyperosmotic exposure (400–450 mOsM)-induced release of cytochrome C. (A) Cytosolic fractions; (B) Mitochondrial fractions (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on the expression of Nrf2.

<p>Levels of Nrf2 in whole cell extracts were determined by the western blot analysis (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on Nrf2 target gene expression in primary HCEpiCs.

<p>Cells were treated with edaravone for 24 h. Gene expression was determined by real-time PCR analysis. (A) HO-1 mRNA; (B) GPx-1 mRNA; (C) GCLC mRNA; (D) GSH levels (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on cleaved caspase-3 as measured by western blot analysis.

<p>Edaravone treatment inhibits the increase in cleaved caspase-3 induced by hyperosmotic exposure (400–450 mOsM) (ANOVA:*, P<0.001 vs. non-treatment control; #, P<0.001 vs. 450 mOsM treated group).</p

Effects of edaravone on apoptosis in primary HCEpiCs exposed to hyperosmotic media (400–450 mOsM).

<p>Apoptotic cells were stained using a TUNEL Assay Kit. Nuclear DNA was stained with DAPI.</p

Edaravone prevents cell death induced by exposure to hyperosmotic media (400–450 mOsM).

<p>(A) Cell viability was measured by MTT assay; (B) Cell death was measured by LDH release assay. All experiments were repeated at least three times (ANOVA: *, P<0.001 vs. untreated control; #, P<0.001 vs. 450 mOsM treated group).</p

Supplemental Material - Endoscopic Lumbar Interbody Fusion, Minimally Invasive Transforaminal Lumbar Interbody Fusion, and Open Transforaminal Lumbar Interbody Fusion for the Treatment of Lumbar Degenerative Diseases: A Systematic Review and Network Meta-Analysis

Supplemental Material for Endoscopic Lumbar Interbody Fusion, Minimally Invasive Transforaminal Lumbar Interbody Fusion, and Open Transforaminal Lumbar Interbody Fusion for the Treatment of Lumbar Degenerative Diseases: A Systematic Review and Network Meta-Analysis by Xijian Hu, Lei Yan, Xinjie Jin, Haifeng Liu, Jing Chai, and Bin Zhao in Global Spine Journal</p