468 research outputs found
A rapid and accurate approach to identify single nucleotide polymorphisms of mitochondrial DNA using MALDI-TOF mass spectrometry
Background: Single nucleotide polymorphisms (SNPs) of mitochondrial DNA (mtDNA) are involved in physiological and pathological conditions. We developed a rapid, accurate, highly sensitive and high-throughput approach with low cost to identify mtDNA SNPs. Methods: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to detect 18 SNPs of mtDNA by uniplex and multiplex assays. The sensitivity and specificity of the MALDI-TOF MS were evaluated. The accuracy of the approach was validated by the comparison of using the robust sequencing analysis. Results: The detection limit achieved with the assays corresponded to the identification of five-genome equivalence of mtDNA per reaction after first round PCR amplification. The testing system enabled the discrimination of as little as 5% of mtDNA polymorphism in the predominating background of mtDNA not containing the SNP. No false positive and false negative results were obtained using the uniplex and multiplex MALDI-TOF MS assays for the analysis of the 18 SNPs compared with those obtained by sequencing analysis. Conclusions: Possible fields which could benefit from this powerful and sensitive tool include forensic medicine, tracing of matrilineage, transplantation immunology, transfusion medicine, the diagnosis of mtDNA mutation related disorders, and the research regarding aging, apoptosis and carcinogenesis based on physiologic and pathogenic alterations of mtDNA for the analysis of large-scale samples, multiple SNPs or rare mtDNA. Clin Chem Lab Med 2008;46:299-30
Coherent manipulation of atomic qubits in optical micropotentials
We experimentally demonstrate the coherent manipulation of atomic states in
far-detuned dipole traps and registers of dipole traps based on two-dimensional
arrays of microlenses. By applying Rabi, Ramsey, and spin-echo techniques, we
systematically investigate the dephasing mechanisms and determine the coherence
time. Simultaneous Ramsey measurements in up to 16 dipole traps are performed
and proves the scalability of our approach. This represents an important step
in the application of scalable registers of atomic qubits for quantum
information processing. In addition, this system can serve as the basis for
novel atomic clocks making use of the parallel operation of a large number of
individual clocks each remaining separately addressable.Comment: to be published in Appl. Phys.
Fast Diffusion Process in Quenched hcp Dilute Solid He-He Mixture
The study of phase structure of dilute He - He solid mixture of
different quality is performed by spin echo NMR technique. The diffusion
coefficient is determined for each coexistent phase. Two diffusion processes
are observed in rapidly quenched (non-equilibrium) hcp samples: the first
process has a diffusion coefficient corresponding to hcp phase, the second one
has huge diffusion coefficient corresponding to liquid phase. That is evidence
of liquid-like inclusions formation during fast crystal growing. It is
established that these inclusions disappear in equilibrium crystals after
careful annealing.Comment: 7 pages, 3 figures, QFS200
Optically induced coherent intra-band dynamics in disordered semiconductors
On the basis of a tight-binding model for a strongly disordered semiconductor
with correlated conduction- and valence band disorder a new coherent dynamical
intra-band effect is analyzed. For systems that are excited by two, specially
designed ultrashort light-pulse sequences delayed by tau relatively to each
other echo-like phenomena are predicted to occur. In addition to the inter-band
photon echo which shows up at exactly t=2*tau relative to the first pulse, the
system responds with two spontaneous intra-band current pulses preceding and
following the appearance of the photon echo. The temporal splitting depends on
the electron-hole mass ratio. Calculating the population relaxation rate due to
Coulomb scattering, it is concluded that the predicted new dynamical effect
should be experimentally observable in an interacting and strongly disordered
system, such as the Quantum-Coulomb-Glass.Comment: to be published in Physical Review B15 February 200
Radiative corrections to scalar-fermion pair production in high energy e+e- collisions
We study the one-loop radiative corrections to pair production of the
supersymmetric scalar partners of the standard fermions in e+e- annihilation.
Both electroweak and SUSY-QCD corrections are considered. Applications are for
production of scalar fermions of the third generation, e^+e^-\to \wt{f}_i
\wt{f}_j^* (i,j=1,2), , as well as for production of scalar
quarks of the first and second generation. Effects on integrated cross sections
are discussed and also the one-loop induced forward-backward asymmetries are
studied. It is found that at low energy, \sqrt{s}\approx 500 \to 1000 GeV, the
corrections are dominated by the QCD contributions, At high energy,
TeV, the electroweak box diagrams give a substantial
contribution and even dominate in some regions of parameters space. The purely
loop-induced forward-backward asymmetry can reach values of several per cent.Comment: 23 pages, latex, 13 figure
Supersymmetric effects in top quark decay into polarized W-boson
We investigate the one-loop supersymmetric QCD (SUSY-QCD) and electroweak
(SUSY-EW) corrections to the top quark decay into a b-quark and a longitudinal
or transverse W-boson. The corrections are presented in terms of the
longitudinal ratio \Gamma(t-->W_L b)/\Gamma(t--> W b) and the transverse ratio
\Gamma(t-->W_- b)/\Gamma(t--> W b). In most of the parameter space, both
SUSY-QCD and SUSY-EW corrections to these ratios are found to be less than 1%
in magnitude and they tend to have opposite signs. The corrections to the total
width \Gamma(t-->W b) are also presented for comparison with the existing
results in the literature. We find that our SUSY-EW corrections to the total
width differ significantly from previous studies: the previous studies give a
large correction of more than 10% in magnitude for a large part of the
parameter space while our results reach only few percent at most.Comment: Version in PRD (explanation and refs added
Semiparametric theory and empirical processes in causal inference
In this paper we review important aspects of semiparametric theory and
empirical processes that arise in causal inference problems. We begin with a
brief introduction to the general problem of causal inference, and go on to
discuss estimation and inference for causal effects under semiparametric
models, which allow parts of the data-generating process to be unrestricted if
they are not of particular interest (i.e., nuisance functions). These models
are very useful in causal problems because the outcome process is often complex
and difficult to model, and there may only be information available about the
treatment process (at best). Semiparametric theory gives a framework for
benchmarking efficiency and constructing estimators in such settings. In the
second part of the paper we discuss empirical process theory, which provides
powerful tools for understanding the asymptotic behavior of semiparametric
estimators that depend on flexible nonparametric estimators of nuisance
functions. These tools are crucial for incorporating machine learning and other
modern methods into causal inference analyses. We conclude by examining related
extensions and future directions for work in semiparametric causal inference
Universality of the Lyapunov regime for the Loschmidt echo
The Loschmidt echo (LE) is a magnitude that measures the sensitivity of
quantum dynamics to perturbations in the Hamiltonian. For a certain regime of
the parameters, the LE decays exponentially with a rate given by the Lyapunov
exponent of the underlying classically chaotic system. We develop a
semiclassical theory, supported by numerical results in a Lorentz gas model,
which allows us to establish and characterize the universality of this Lyapunov
regime. In particular, the universality is evidenced by the semiclassical limit
of the Fermi wavelength going to zero, the behavior for times longer than
Ehrenfest time, the insensitivity with respect to the form of the perturbation
and the behavior of individual (non-averaged) initial conditions. Finally, by
elaborating a semiclassical approximation to the Wigner function, we are able
to distinguish between classical and quantum origin for the different terms of
the LE. This approach renders an understanding for the persistence of the
Lyapunov regime after the Ehrenfest time, as well as a reinterpretation of our
results in terms of the quantum--classical transition.Comment: 33 pages, 17 figures, uses Revtex
Predictive powers of chiral perturbation theory in Compton scattering off protons
We study low-energy nucleon Compton scattering in the framework of baryon
chiral perturbation theory (BPT) with pion, nucleon, and (1232)
degrees of freedom, up to and including the next-to-next-to-leading order
(NNLO). We include the effects of order , and , with
MeV the -resonance excitation energy. These are
all "predictive" powers in the sense that no unknown low-energy constants enter
until at least one order higher (i.e, ). Estimating the theoretical
uncertainty on the basis of natural size for effects, we find that
uncertainty of such a NNLO result is comparable to the uncertainty of the
present experimental data for low-energy Compton scattering. We find an
excellent agreement with the experimental cross section data up to at least the
pion-production threshold. Nevertheless, for the proton's magnetic
polarizability we obtain a value of fm, in
significant disagreement with the current PDG value. Unlike the previous
PT studies of Compton scattering, we perform the calculations in a
manifestly Lorentz-covariant fashion, refraining from the heavy-baryon (HB)
expansion. The difference between the lowest order HBPT and BPT
results for polarizabilities is found to be appreciable. We discuss the chiral
behavior of proton polarizabilities in both HBPT and BPT with the
hope to confront it with lattice QCD calculations in a near future. In studying
some of the polarized observables, we identify the regime where their naive
low-energy expansion begins to break down, thus addressing the forthcoming
precision measurements at the HIGS facility.Comment: 24 pages, 9 figures, RevTeX4, revised version published in EPJ
Molecular velocity auto-correlation of simple liquids observed by NMR MGSE method
The velocity auto-correlation spectra of simple liquids obtained by the NMR
method of modulated gradient spin echo show features in the low frequency range
up to a few kHz, which can be explained reasonably well by a long
time tail decay only for non-polar liquid toluene, while the spectra of polar
liquids, such as ethanol, water and glycerol, are more congruent with the model
of diffusion of particles temporarily trapped in potential wells created by
their neighbors. As the method provides the spectrum averaged over ensemble of
particle trajectories, the initial non-exponential decay of spin echoes is
attributed to a spatial heterogeneity of molecular motion in a bulk of liquid,
reflected in distribution of the echo decays for short trajectories. While at
longer time intervals, and thus with longer trajectories, heterogeneity is
averaged out, giving rise to a spectrum which is explained as a combination of
molecular self-diffusion and eddy diffusion within the vortexes of hydrodynamic
fluctuations.Comment: 8 pages, 6 figur
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