Preparation and evaluation of the effect of Fe3O4@piroctone olamine magnetic nanoparticles on matrix metalloproteinase-2: A preliminary in vitro study

In the present study, Fe3O4 magnetic nanoparticles were synthesized by the coprecipitation of Fe2+ and Fe3+ ions and used as a nanocarrier for the production of piroctoneolamine- loaded Fe3O4 nanoparticles (Fe3O4@PO NPs). The nanocrystalline structure of the prepared iron oxide species was confirmed by the X-ray diffraction spectroscopy method. Particle size distribution analysis showed that the size of Fe3O4@PO NPs was in the range of 5–55 nm. The magnetization curve of Fe3O4@PO NPs (with saturation magnetization of 28.2 emu/g) confirmed its ferromagnetic property. Loading of PO on the surface of Fe3O4 NPs qualitatively verified by Fourier transform infrared spectrum obtained from Fe3O4@PO NPs. Cytotoxicity studies on the human fibrosarcoma cell line (HT-1080) revealed higher inhibitory effect of Fe3O4@PO NPs (50% cell death [IC50] of 8.1 μg/mL) as compared with Fe3O4 NPs (IC50 of 117.1 μg/mL) and PO (IC50 of 71.2 μg/mL) alone. In the case of human normal fibroblast (Hs68), the viability percentage was found to be 75% in the presence of Fe3O4@PO NPs (120 μg/mL). Gelatin zymography showed 17.2% and 34.6% inhibition of matrix metalloproteinase-2 (MMP-2) in the presence of Fe3O4@PO and PO, respectively, at the same concentration of 40 μg/mL, whereas Fe3O4 NPs did not inhibit MMP-2 at any concentration. C� 2014 International Union of Biochemistry and Molecular Biology, Inc. Volume 61, Number 6, Pages 676–682, 201