Preparation and evaluation of the effect
of Fe3O4@piroctone olamine magnetic
nanoparticles on matrix metalloproteinase-2:
A preliminary in vitro study
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Abstract
In the present study, Fe3O4 magnetic nanoparticles were
synthesized by the coprecipitation of Fe2+ and Fe3+ ions and
used as a nanocarrier for the production of piroctoneolamine-
loaded Fe3O4 nanoparticles (Fe3O4@PO NPs). The
nanocrystalline structure of the prepared iron oxide species
was confirmed by the X-ray diffraction spectroscopy method.
Particle size distribution analysis showed that the size of
Fe3O4@PO NPs was in the range of 5–55 nm. The
magnetization curve of Fe3O4@PO NPs (with saturation
magnetization of 28.2 emu/g) confirmed its ferromagnetic
property. Loading of PO on the surface of Fe3O4 NPs
qualitatively verified by Fourier transform infrared spectrum
obtained from Fe3O4@PO NPs. Cytotoxicity studies on the
human fibrosarcoma cell line (HT-1080) revealed higher
inhibitory effect of Fe3O4@PO NPs (50% cell death [IC50] of
8.1 μg/mL) as compared with Fe3O4 NPs (IC50 of 117.1 μg/mL)
and PO (IC50 of 71.2 μg/mL) alone. In the case of human
normal fibroblast (Hs68), the viability percentage was found to
be 75% in the presence of Fe3O4@PO NPs (120 μg/mL). Gelatin
zymography showed 17.2% and 34.6% inhibition of matrix
metalloproteinase-2 (MMP-2) in the presence of Fe3O4@PO
and PO, respectively, at the same concentration of 40 μg/mL,
whereas Fe3O4 NPs did not inhibit MMP-2 at any
concentration. C� 2014 International Union of Biochemistry and
Molecular Biology, Inc. Volume 61, Number 6, Pages 676–682, 201