A chip calorimetry-based method for the real-time investigation of metabolic activity changes in human erythrocytes caused by cell sickling

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOA new calorimetric technique is described which allows the measurement of metabolic heat rates in biological materials which are triggered by changes in the oxygen tension. It uses the high permeability for oxygen of a thin-walled Teflon flow channel of a136771781FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO2014/00984-3The authors are indebted to FAPESP – Fundação de Amparo à Pesquisa do Estado de São Paulo, Brasil for financial support (2014/00984-3) and Hematology and Hemotherapy Center –Hemocentro Campinas for supplying the samples. We would like to thank Dominique

Radiometric and angular calibration tests for the MEDA-TIRS radiometer onboard NASA's Mars 2020 mission

This article describes a comprehensive testing method for the radiometric and angular calibration of the Thermal InfraRed Sensor (TIRS) onboard NASA's Mars 2020 mission. First, details of the TIRS opto-mechanical design, construction aspects of the IR detectors, and an update of the mathematical model used for the calculation of sensor internal IR fluxes are provided. Then, a set of sequential calibration tests to identify the radiometer model parameters are defined. The test setups are described, highlighting their limitations and restrictions based on differences between simulated and actual Martian environmental conditions. Finally, the uncertainty sources and potential systematic errors associated with the calibration tests are quantified and compared with the radiometer scientific requirements established by the Mars 2020 science team.With funding from the Spanish government through the "María de Maeztu Unit of Excellence" accreditation (MDM-2017-0737

A chip calorimetry-based method for the real-time investigation of metabolic activity changes in human erythrocytes caused by cell sickling

A new calorimetric technique is described which allows the measurement of metabolic heat rates in biological materials which are triggered by changes in the oxygen tension. It uses the high permeability for oxygen of a thin-walled Teflon flow channel of a chip calorimeter in order to adjust variable but defined oxygen concentrations in the samples. Using this technique, for the first time, the heat production rate of sickle cell erythrocytes (SS-RBCs) in sickled and non-sickled state could be compared. To measure heat rate changes in real time when sickled SS-RBCs return to the non-sickled state, the heat dissipation was measured during the re-oxygenation of the cell samples which had been treated before under anoxic conditions. We found higher heat production rate of the blood cells in non-sickled state, which is in agreement with the reported increased glucose uptake1362771781FAPESP – Fundação de Amparo à Pesquisa Do Estado De São Paulo2014/00984-