2,764 research outputs found
Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts.
Pancreatic cancer is a complex disease, in need of new therapeutic approaches. In this study, we explored the effect and mechanism of action of epigallocatechin-3-gallate (EGCG), a major polyphenol in green tea, alone and in combination with current chemotherapeutics on pancreatic cancer cell growth, focusing on glycolysis metabolism. Moreover, we investigated whether EGCG's effect is dependent on its ability to induce reactive oxygen species (ROS). EGCG reduced pancreatic cancer cell growth in a concentration-dependent manner and the growth inhibition effect was further enhanced under glucose deprivation conditions. Mechanistically, EGCG induced ROS levels concentration-dependently. EGCG affected glycolysis by suppressing the extracellular acidification rate through the reduction of the activity and levels of the glycolytic enzymes phosphofructokinase and pyruvate kinase. Cotreatment with catalase abrogated EGCG's effect on phosphofructokinase and pyruvate kinase. Furthermore, EGCG sensitized gemcitabine to inhibit pancreatic cancer cell growth in vitro and in vivo. EGCG and gemcitabine, given alone, reduced pancreatic tumor xenograft growth by 40% and 52%, respectively, whereas the EGCG/gemcitabine combination reduced tumor growth by 67%. EGCG enhanced gemcitabine's effect on apoptosis, cell proliferation, cell cycle and further suppressed phosphofructokinase and pyruvate kinase levels. In conclusion, EGCG is a strong combination partner of gemcitabine reducing pancreatic cancer cell growth by suppressing glycolysis
Spot activity of the RS CVn star {\sigma} Geminorum
We model the photometry of RS CVn star Geminorum to obtain new
information on the changes of the surface starspot distribution, i.e., activity
cycles, differential rotation and active longitudes. We use the previously
published Continuous Periods Search-method (CPS) to analyse V-band differential
photometry obtained between the years 1987 and 2010 with the T3 0.4 m Automated
Telescope at the Fairborn Observatory. The CPS-method divides data into short
subsets and then models the light curves with Fourier-models of variable orders
and provides estimates of the mean magnitude, amplitude, period and light curve
minima. These light curve parameters are then analysed for signs of activity
cycles, differential rotation and active longitudes. We confirm the presence of
two previously found stable active longitudes, synchronised with the orbital
period d and find eight events where the active longitudes
are disrupted. The epochs of the primary light curve minima rotate with a
shorter period d than the orbital motion. If the
variations in the photometric rotation period were to be caused by differential
rotation, this would give a differential rotation coefficient of . The presence of two slightly different periods of active regions may
indicate a superposition of two dynamo modes, one stationary in the orbital
frame and the other one propagating in the azimuthal direction. Our estimate of
the differential rotation is much higher than previous results. However,
simulations show that this can be caused by insufficient sampling in our data.Comment: 10 pages, 6 figures. Submitted to A&
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Epigallocatechin-3-Gallate (EGCG) Suppresses Pancreatic Cancer Cell Growth, Invasion, and Migration partly through the Inhibition of Akt Pathway and Epithelial-Mesenchymal Transition: Enhanced Efficacy when Combined with Gemcitabine.
Most pancreatic cancers are usually diagnosed at an advanced stage when they have already metastasized. Epigallocatechin-3-gallate (EGCG), a major polyphenolic constituent of green tea, has been shown to reduce pancreatic cancer growth, but its effect on metastasis remains elusive. This study evaluated the capacity of EGCG to inhibit pancreatic cancer cell migration and invasion and the underlying mechanisms. EGCG reduced pancreatic cancer cell growth, migration, and invasion in vitro and in vivo. EGCG prevented "Cadherin switch" and decreased the expression level of TCF8/ZEB1, β-Catenin, and Vimentin. Mechanistically, EGCG inhibited the Akt pathway in a time-dependent manner, by suppressing IGFR phosphorylation and inducing Akt degradation. Co-treatment with catalase or N-Acetyl-L-cysteine did not abrogate EGCG's effect on the Akt pathway or cell growth. Moreover, EGCG synergized with gemcitabine to suppress pancreatic cancer cell growth, migration, and invasion, through modulating epithelial-mesenchymal transition markers and inhibiting Akt pathway. In summary, EGCG may prove beneficial to improve gemcitabine sensitivity in inhibiting pancreatic cancer cell migration and invasion, to some extent through the inhibition of Akt pathway and epithelial-mesenchymal transition
Stellar activity as noise in exoplanet detection I. Methods and application to solar-like stars and activity cycles
The detection of exoplanets using any method is prone to confusion due to the
intrinsic variability of the host star. We investigate the effect of cool
starspots on the detectability of the exoplanets around solar-like stars using
the radial velocity method. For investigating this activity-caused "jitter" we
calculate synthetic spectra using radiative transfer, known stellar atomic and
molecular lines, different surface spot configurations, and an added planetary
signal. Here, the methods are described in detail, tested and compared to
previously published studies. The methods are also applied to investigate the
activity jitter in old and young solar-like stars, and over a solar-like
activity cycles. We find that the mean full jitter amplitude obtained from the
spot surfaces mimicking the solar activity varies during the cycle
approximately between 1 m/s and 9 m/s. With a realistic observing frequency a
Neptune mass planet on a one year orbit can be reliably recovered. On the other
hand, the recovery of an Earth mass planet on a similar orbit is not feasible
with high significance. The methods developed in this study have a great
potential for doing statistical studies of planet detectability, and also for
investigating the effect of stellar activity on recovered planetary parameters.Comment: Accepted to MNRA
First measurement of the magnetic field on FK Com and its relation to the contemporaneous starspot locations
In this study we present simultaneous low-resolution longitudinal magnetic
field measurements and high-resolution spectroscopic observations of the cool
single giant FK Com. The variation of the magnetic field over the rotational
period of 2.4 days is compared with the starspot location obtained using
Doppler imaging techniques, V-band photometry and V-I colours. The
chromospheric activity is studied simultaneously with the photospheric activity
using high resolution observations of the Halpha, Hbeta and Hgamma line
profiles. Both the maximum (272 +/- 24 G) and minimum (60 +/- 17 G) in the mean
longitudinal magnetic field, , are detected close to the phases where cool
spots appear on the stellar surface. A possible explanation for such a
behaviour is that the active regions at the two longitudes separated by 0.2 in
phase have opposite polarities.Comment: 10 Pages, 11 figures (quality of Figures 7,8 and 10 reduced),
accepted for publication in MNRA
Ellipsoidal primary of the RS CVn binary zeta And: Investigation using high-resolution spectroscopy and optical interferometry
We have obtained high-resolution spectroscopy, optical interferometry, and
long-term broad band photometry of the ellipsoidal primary of the RS CVn-type
binary system zeta And. Based on the optical interferometry the apparent limb
darkened diameter of zeta And is 2.55 +/- 0.09 mas using a uniform disk fit.
The Hipparcos distance and the limb-darkened diameter obtained with a uniform
disk fit give stellar radius of 15.9 +/- 0.8 Rsolar, and combined with
bolometric luminosity, it implies an effective temperature of 4665 +/- 140 K.
The temperature maps obtained from high resolution spectra using Doppler
imaging show a strong belt of equatorial spots and hints of a cool polar cap.
The equatorial spots show a concentration around the phase 0.75. This spot
configuration is reminiscent of the one seen in the earlier published
temperature maps of zeta And. Investigation of the Halpha line reveals both
prominences and cool clouds in the chromosphere. Long-term photometry spanning
12 years shows hints of a spot activity cycle, which is also implied by the
Doppler images, but the cycle length cannot be reliably determined from the
current data.Comment: 9 pages, 9 figures, accepted for A&
Quality Assessment Of Aqueous Herbal/Medicinal Products Sold On The Ghanaian Market.
The microbial quality of sixteen (16) decoctions sold on the Ghanaian market were investigated and the isolated microbes characterized to the species level. The results indicated that, almost all the decoctions were contaminated with aerobic bacteria and/or fungi. The highest microbial counts greater than 1.0x109 cfu/ml were found in three of the samples with only one out of the sixteen samples having the lowest aerobic bacterial count of 1.0x102 cfu/ml. Fungal contaminations were found in thirteen (81.3%) of the samples with only three found to be free from fungal contamination. The highest fungal contamination of 3.2x105 cfu/ml was found in only one sample. The characterization of the isolates revealed six bacterial genera and eight fungal genera with Bacillus subtilis (50.0%) and Cladosporium herbarum (34.5%) being the predominant bacterial and fungal isolates respectively. The analysis of the samples after three months storage showed that the microbial load of the decoctions were within the acceptable limits whilst the microbial isolates also showed a reduction for B. subtilis (23.8%) and C. herbarum (14.3%). The study revealed that most of the decoctions sold on the Ghanaian market are contaminated with both bacteria and fungi. Key words: herbal medicine, aerobic plate count, spoilage microbes, microbial characterizatio
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