Targeting Glycolysis with Epigallocatechin-3-Gallate Enhances the Efficacy of Chemotherapeutics in Pancreatic Cancer Cells and Xenografts.

Pancreatic cancer is a complex disease, in need of new therapeutic approaches. In this study, we explored the effect and mechanism of action of epigallocatechin-3-gallate (EGCG), a major polyphenol in green tea, alone and in combination with current chemotherapeutics on pancreatic cancer cell growth, focusing on glycolysis metabolism. Moreover, we investigated whether EGCG's effect is dependent on its ability to induce reactive oxygen species (ROS). EGCG reduced pancreatic cancer cell growth in a concentration-dependent manner and the growth inhibition effect was further enhanced under glucose deprivation conditions. Mechanistically, EGCG induced ROS levels concentration-dependently. EGCG affected glycolysis by suppressing the extracellular acidification rate through the reduction of the activity and levels of the glycolytic enzymes phosphofructokinase and pyruvate kinase. Cotreatment with catalase abrogated EGCG's effect on phosphofructokinase and pyruvate kinase. Furthermore, EGCG sensitized gemcitabine to inhibit pancreatic cancer cell growth in vitro and in vivo. EGCG and gemcitabine, given alone, reduced pancreatic tumor xenograft growth by 40% and 52%, respectively, whereas the EGCG/gemcitabine combination reduced tumor growth by 67%. EGCG enhanced gemcitabine's effect on apoptosis, cell proliferation, cell cycle and further suppressed phosphofructokinase and pyruvate kinase levels. In conclusion, EGCG is a strong combination partner of gemcitabine reducing pancreatic cancer cell growth by suppressing glycolysis

Spot activity of the RS CVn star {\sigma} Geminorum

We model the photometry of RS CVn star $\sigma$ Geminorum to obtain new information on the changes of the surface starspot distribution, i.e., activity cycles, differential rotation and active longitudes. We use the previously published Continuous Periods Search-method (CPS) to analyse V-band differential photometry obtained between the years 1987 and 2010 with the T3 0.4 m Automated Telescope at the Fairborn Observatory. The CPS-method divides data into short subsets and then models the light curves with Fourier-models of variable orders and provides estimates of the mean magnitude, amplitude, period and light curve minima. These light curve parameters are then analysed for signs of activity cycles, differential rotation and active longitudes. We confirm the presence of two previously found stable active longitudes, synchronised with the orbital period $P_{\rm{orb}}=19.60$d and find eight events where the active longitudes are disrupted. The epochs of the primary light curve minima rotate with a shorter period $P_{\rm{min,1}}=19.47$d than the orbital motion. If the variations in the photometric rotation period were to be caused by differential rotation, this would give a differential rotation coefficient of $\alpha \ge 0.103$. The presence of two slightly different periods of active regions may indicate a superposition of two dynamo modes, one stationary in the orbital frame and the other one propagating in the azimuthal direction. Our estimate of the differential rotation is much higher than previous results. However, simulations show that this can be caused by insufficient sampling in our data.Comment: 10 pages, 6 figures. Submitted to A&

Epigallocatechin-3-Gallate (EGCG) Suppresses Pancreatic Cancer Cell Growth, Invasion, and Migration partly through the Inhibition of Akt Pathway and Epithelial-Mesenchymal Transition: Enhanced Efficacy when Combined with Gemcitabine.

Most pancreatic cancers are usually diagnosed at an advanced stage when they have already metastasized. Epigallocatechin-3-gallate (EGCG), a major polyphenolic constituent of green tea, has been shown to reduce pancreatic cancer growth, but its effect on metastasis remains elusive. This study evaluated the capacity of EGCG to inhibit pancreatic cancer cell migration and invasion and the underlying mechanisms. EGCG reduced pancreatic cancer cell growth, migration, and invasion in vitro and in vivo. EGCG prevented "Cadherin switch" and decreased the expression level of TCF8/ZEB1, β-Catenin, and Vimentin. Mechanistically, EGCG inhibited the Akt pathway in a time-dependent manner, by suppressing IGFR phosphorylation and inducing Akt degradation. Co-treatment with catalase or N-Acetyl-L-cysteine did not abrogate EGCG's effect on the Akt pathway or cell growth. Moreover, EGCG synergized with gemcitabine to suppress pancreatic cancer cell growth, migration, and invasion, through modulating epithelial-mesenchymal transition markers and inhibiting Akt pathway. In summary, EGCG may prove beneficial to improve gemcitabine sensitivity in inhibiting pancreatic cancer cell migration and invasion, to some extent through the inhibition of Akt pathway and epithelial-mesenchymal transition

Stellar activity as noise in exoplanet detection I. Methods and application to solar-like stars and activity cycles

The detection of exoplanets using any method is prone to confusion due to the intrinsic variability of the host star. We investigate the effect of cool starspots on the detectability of the exoplanets around solar-like stars using the radial velocity method. For investigating this activity-caused "jitter" we calculate synthetic spectra using radiative transfer, known stellar atomic and molecular lines, different surface spot configurations, and an added planetary signal. Here, the methods are described in detail, tested and compared to previously published studies. The methods are also applied to investigate the activity jitter in old and young solar-like stars, and over a solar-like activity cycles. We find that the mean full jitter amplitude obtained from the spot surfaces mimicking the solar activity varies during the cycle approximately between 1 m/s and 9 m/s. With a realistic observing frequency a Neptune mass planet on a one year orbit can be reliably recovered. On the other hand, the recovery of an Earth mass planet on a similar orbit is not feasible with high significance. The methods developed in this study have a great potential for doing statistical studies of planet detectability, and also for investigating the effect of stellar activity on recovered planetary parameters.Comment: Accepted to MNRA

First measurement of the magnetic field on FK Com and its relation to the contemporaneous starspot locations

In this study we present simultaneous low-resolution longitudinal magnetic field measurements and high-resolution spectroscopic observations of the cool single giant FK Com. The variation of the magnetic field over the rotational period of 2.4 days is compared with the starspot location obtained using Doppler imaging techniques, V-band photometry and V-I colours. The chromospheric activity is studied simultaneously with the photospheric activity using high resolution observations of the Halpha, Hbeta and Hgamma line profiles. Both the maximum (272 +/- 24 G) and minimum (60 +/- 17 G) in the mean longitudinal magnetic field, , are detected close to the phases where cool spots appear on the stellar surface. A possible explanation for such a behaviour is that the active regions at the two longitudes separated by 0.2 in phase have opposite polarities.Comment: 10 Pages, 11 figures (quality of Figures 7,8 and 10 reduced), accepted for publication in MNRA

Ellipsoidal primary of the RS CVn binary zeta And: Investigation using high-resolution spectroscopy and optical interferometry

We have obtained high-resolution spectroscopy, optical interferometry, and long-term broad band photometry of the ellipsoidal primary of the RS CVn-type binary system zeta And. Based on the optical interferometry the apparent limb darkened diameter of zeta And is 2.55 +/- 0.09 mas using a uniform disk fit. The Hipparcos distance and the limb-darkened diameter obtained with a uniform disk fit give stellar radius of 15.9 +/- 0.8 Rsolar, and combined with bolometric luminosity, it implies an effective temperature of 4665 +/- 140 K. The temperature maps obtained from high resolution spectra using Doppler imaging show a strong belt of equatorial spots and hints of a cool polar cap. The equatorial spots show a concentration around the phase 0.75. This spot configuration is reminiscent of the one seen in the earlier published temperature maps of zeta And. Investigation of the Halpha line reveals both prominences and cool clouds in the chromosphere. Long-term photometry spanning 12 years shows hints of a spot activity cycle, which is also implied by the Doppler images, but the cycle length cannot be reliably determined from the current data.Comment: 9 pages, 9 figures, accepted for A&

Quality Assessment Of Aqueous Herbal/Medicinal Products Sold On The Ghanaian Market.

The microbial quality of sixteen (16) decoctions sold on the Ghanaian market were investigated and the isolated microbes characterized to the species level. The results indicated that, almost all the decoctions were contaminated with aerobic bacteria and/or fungi. The highest microbial counts greater than 1.0x109 cfu/ml were found in three of the samples with only one out of the sixteen samples having the lowest aerobic bacterial count of 1.0x102 cfu/ml. Fungal contaminations were found in thirteen (81.3%) of the samples with only three found to be free from fungal contamination. The highest fungal contamination of 3.2x105 cfu/ml was found in only one sample. The characterization of the isolates revealed six bacterial genera and eight fungal genera with Bacillus subtilis (50.0%) and Cladosporium herbarum (34.5%) being the predominant bacterial and fungal isolates respectively. The analysis of the samples after three months storage showed that the microbial load of the decoctions were within the acceptable limits whilst the microbial isolates also showed a reduction for B. subtilis (23.8%) and C. herbarum (14.3%). The study revealed that most of the decoctions sold on the Ghanaian market are contaminated with both bacteria and fungi. Key words: herbal medicine, aerobic plate count, spoilage microbes, microbial characterizatio