14 research outputs found
Production of a new anti-A monoclonal reagent
Monoclonal antibodies are essential tools in molecular and cellular immunology research. They have essentially replaced the polyclonal antibodies in identifying blood groups and detecting cell markers and pathogenic agents. The aim of the present study is to produce monoclonal antibody identifying the ABO blood groups using the murine hybridoma technology. An anti-A monoclonal antibody A907 was selected and estimated for its use in the manufacture of a reagent anti-A. The selected antibody specifically reacts with A1, A2/sub>, A1B and A2B erythrocytes. It does not recognize B, O, A3 and Ax erythrocytes. The A907 monoclonal antibody can be used in blood grouping in association with a reagent recognizing the A weak phenotypes.Key words: Anti-A monoclonal antibodies, murine myeloma cells, haemagglutination.African Journal of Biotechnology Vol. 4 (8), pp. 844-84
Key Concepts of Psychotherapy on the Basis of Play Therapy
Effect of Allium sativum extract on human PBMC proliferation. CD4 T cell division was evaluated by CFSE staining and flow cytometry when PBMCs were treated with or without A.S. at two different doses in the absence or presence of PHA (a) or Okt-3 mAb (b). CD8 T cell division assessed by CFSE staining and flux cytometry within PBMCs treated with or without A.S. at two doses in the absence or presence of PHA (c) or Okt-3 mAb (d). Data shown are representative of 4 independent experiments. Percentage of cell division was calculated with FlowJo software. Data are represented in meanāĀ±āS.D. and were analyzed using the one-way ANOVA test. (DOCX 278 kb
Interactions cellulaires et activation des lymphocytes T: etude a l'aide d'anticorps monoclonaux
SIGLEAvailable from INIST (FR), Document Supply Service, under shelf-number : T 81946 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc
In Vitro and In Vivo Immunomodulator Activities of Allium sativum L.
Allium Sativum L. (garlic), which is a species of the onion family, Alliaceae, is one of the most used plants in traditional medicine worldwide. More than 200 chemicals with diverse properties have been found in garlic extracts. Several garlic compounds were suggested to be efficient in improving various pathologies including certain types of cancer. This paper is an overview of data about garlic biological activities in vitro and/or in vivo on immune cells, on the development of certain inflammatory diseases, and on different types of carcinomas and sarcomas. Garlic and its compounds were found to have notable antioxidant properties. Garlic therapeutic potential has also been studied in several inflammatory diseases such as allergic-airway inflammation, inflammatory bowel disease, arthritic rheumatism, and atherosclerosis. Furthermore, garlic was found to be able to maintain the immune system homeostasis and to exhibit beneficial effects on immune cells especially through regulation of proliferation and cytokine gene expression. Finally, we will show how major garlic components such as sulfur compounds and polyphenols might be responsible for the garlic biological activities revealed in different situations. If identified, specific compounds present in garlic could potentially be used in therapy
Anti-inflammatory potential of Capparis spinosa L. in vivo in mice through inhibition of cell infiltration and cytokine gene expression
Abstract
Background
Several chronic inflammatory diseases are characterized by inappropriate CD4+ T cell response. In the present study, we assessed the ability of Capparis spinosa L. (CS) preparation to orientate, in vivo, the immune response mediated by CD4+ T cells towards an anti-inflammatory response.
Methods
The in vivo study was carried out by using the contact hypersensitivity (CHS) model in Swiss mice. Then we performed a histological analysis followed by molecular study by using real time RT-PCR. We also realized a phytochemical screening and a liquid-liquid separation of CS preparation.
Results
Our study allowed us to detect a significantly reduced edema in mice treated with CS preparations relative to control. CS effect was dose dependent, statistically similar to that observed with indomethacin, independent of the plant genotype and of the period of treatment. Furthermore, our histology studies revealed that CS induced a significant decrease in immune cell infiltration, in vasodilatation and in dermis thickness in the inflammatory site. Interestingly, we showed that CS operated by inhibiting cytokine gene expression including IFN\u3b3, IL-17 and IL-4. Besides, phytochemical screening of CS extract showed the presence of several chemical families such as saponins, flavonoids and alkaloids. One (hexane fraction) out of the three distinct prepared fractions, exhibited an anti-inflammatory effect similar to that of the raw preparation, and would likely contain the bioactive(s) molecule(s).
Conclusions
Altogether, our data indicate that CS regulates inflammation induced in vivo in mice and thus could be a source of anti-inflammatory molecules, which could be used in some T lymphocyte-dependent inflammatory diseases
Additional file 3: of Capparis Spinosa L. promotes anti-inflammatory response in vitro through the control of cytokine gene expression in human peripheral blood mononuclear cells
Raw data Figure 3. (XLSX 10 kb
Additional file 5: Figure S1. of Capparis Spinosa L. promotes anti-inflammatory response in vitro through the control of cytokine gene expression in human peripheral blood mononuclear cells
Effects of Capparis Spinosaās aqueous fraction on IL-4, IL-17, IL-10, TGF-Ī² and TNF-Ī± expressions in stimulated PBMCs with PHA (5Ā Ī¼g/ml) in culture. (G) IL-4, (H) IL-17, (I) IL-10, (J) TGF-Ī², (K) TNF-Ī±. incubated for 18Ā h, doses used 100 and 500Ā Ī¼g/ml. Data represent the meanāĀ±āS.D. Data from nā=ā5 separate experiments. (DOCX 108 kb
Additional file 2: of Capparis Spinosa L. promotes anti-inflammatory response in vitro through the control of cytokine gene expression in human peripheral blood mononuclear cells
Raw data Figure 2. (XLSX 20 kb
Additional file 4: of Capparis Spinosa L. promotes anti-inflammatory response in vitro through the control of cytokine gene expression in human peripheral blood mononuclear cells
Raw data Figure 4 and supplementary figure. (XLSX 50 kb
Additional file 2: of Allium sativum L. regulates in vitro IL-17 gene expression in human peripheral blood mononuclear cells
Raw data of MTT assays. (XLSX 10 kb