Leukemoid reaction in a patient with acute lymphoblastic leukemia following the

The occurrence of persistent neutrophilic leukocytosisabove 50,000 cells/μL for reasons other thanleukemia is defined as leukemoid reaction. Chronicmyelogenous leukemia (CML) and chronic neutrophilicleukemia (CNL) should be excluded, and underlyingdiseases or causes should be examined,in differential diagnosis. The most commonly observedcauses of leukemoid reactions are severeinfections, intoxications, malignancies, severe hemorrhage,or acute hemolysis [1]. J Clin Exp Invest2013; 4 (2): 258-25

Jak-2 mutation frequency in patients with thrombocytosis

Background: We aimed to investigate the etiologic causes and the existence of Janus kinase 2 mutation (JAK2) in cases with thrombocytosis. Methods: In this retrospective study, patients who were admitted to hematology clinic with thrombocytosis between 2013 and 2015 were investigated in terms of the etiological causes of thrombocytosis and the existence of JAK2 mutation. Results: We retrospectively evaluated 136 cases that underwent JAK2 mutation analysis due to ET preliminary diagnosis in our hematology clinic. The mean age of the patients was 56.7±15.3 years (range: 22-86 years) and 71 (52%) were males. JAK2 mutation was found to be positive in 58 (42%) of cases. The mean platelet counts of the cases were 860.25×109 / L (range: 455-1,105 109 /L) and the mean spleen vertical length was 135.1±21.6 mm (range: 110-220 mm). Conclusions: JAK2 mutation analysis and bone marrow biopsy are the two main procedures to diagnose primary thrombocytosis in adults with persistent thrombocytosis after excluding the causes of secondary thrombocytosis. Myeloproliferative neoplasms and essential thrombocytosis are the common causes of primary thrombocytosis in adults with persistent thrombocytosis, as myelodysplastic syndrome rarely causes primary thrombocytosis

A case with acute myeloid leukemia and hepatosplenic Candida parapsilosis

Hepatosplenic candidiasis (HSC) is a disseminated invasive fungal infection that develops commonly in patients with acute leukemia. The main symptom of HSC is a persistent fever in patients who have recovered from prolonged neutropenia subsequent to recent chemotherapy. Herein, a case is presented of a 32-year-old man who was followed up at hematology ward due to acute myeloid leukemia and febrile neutropenia related to cytarabine (ara-C) and daunorubicin chemotherapy. HSC was diagnosed and confirmed by ultrasonography, the culture of blood, and biopsy specimen

Etiological causes of pancytopenia: A report of 137 cases

Objective: The aim of this study is to evaluate the etiological causes of pancytopenia in patients who were admitted to the hematology ward due to pancytopenia. Materials and Methods: All patients who were admitted to the hematology ward of Ministry of Health Istanbul Training and Research Hospital due to pancytopenia between 2013 and 2015 were retrospectively evaluated regarding etiological causes. Results: A total of 137 patients was evaluated. The etiological causes of pancytopenia were recorded as Vitamin B12 deficiency (n: 24; 17%), chronic liver disease (n: 21; 15%), malignancy (n: 19; 13%), myelodysplastic syndrome (n: 18; 13%), aplastic anemia (n: 11; 8%), rheumatic diseases (n: 7; 5%), and endocrine causes (n: 3; 2%). Conclusion: The etiological causes of pancytopenia vary depends on patients′ age, gender, country, and other conditions. Vitamin B12 deficiency is the most common treatable cause of pancytopenia. Most of the etiological causes could be diagnosed with laboratory analysis and radiological imagings, without the need of a bone marrow examination

Janus kinase 2 mutations in cases with BCR-ABL-negative chronic myeloproliferative disorders from Turkey

Objective: We aimed to investigate the frequency of Janus kinase 2 ( JAK2) mutations in cases with chronic myeloproliferative disorders (CMDs), and the relationship between the presence of JAK2 mutation and leukocytosis and splenomegaly, retrospectively. Materials and Methods: Patients, who were diagnosed with BCR-ABL-negative CMDs according to diagnosis criteria of the World Health Organization and followed up at the hematology clinic between 2013 and 2015, were investigated in terms of the frequency of JAK2 mutation in cases with CMDs, and the relationship between the presence of JAK2 mutation and leukocytosis and splenomegaly, retrospectively. Results: In total, 100 patients, who were diagnosed with BCR-ABL-negative CMDs, were evaluated retrospectively. The mean age of the patients with JAK2 positivity was significantly higher compared to patients with negative. JAK2-positivity rates in the age groups were significantly different. Gender, diagnosis, splenomegaly, and leukocytosis were not statistically different for JAK2 positivity between the groups. Conclusion: JAK2 V617F mutation is more commonly seen in older age as a risk for complications related to CDMS. Splenomegaly and leukocytosis are not associated with JAK2 V617F mutation

The Comparison of Cord Blood Omentin-1 Values of Newborns in the Different Birth Weights According to Gestational Age

WOS: 000458736500013Objectives: Our aim in this study was to compare the values of omentin-1, which is an adipokine associated with postnatal metabolic complications, in the cord blood of newborns who were small for gestational age (SGA), appropriate for gestational age (AGA) and large for gestational age (LGA). Methods: This prospective, cross-sectional study was carried out with the measurement of omentin-1 values by Enzyme-Linked Immunosorbent method in the cord blood of the infants who were born at the delivery room of Ministry of Health, Istanbul Okmeydani Training and Research Hospital between 01/08/2016 and 31/01/2017. Results: In the study, omentin-1 values of 30 infants with AGA, 25 infants with LGA and 20 infants with SGA were measured and compared with each other. The mean of cord blood omentin-1 values in the newborn group with SGA was found to be significantly low compared to that of the newborns with AGA. However, no statistically significant difference was found between omentin-1 values of the three groups. Conclusions: We have determined serum omentin-1 levels to be lower in infants with SGA. Metabolic follow-up of infants with SGA is thought to be beneficial because low levels of omentin-1, which is an adipokine in metabolic functions in infants with SGA, may lead to the development of metabolic complications

The Improvement of Endoscope Reprocessing with ATP-Bioluminescence Tool

Background: The incidence rates of endoscopy use associated healthcare-associated infection were reported to be 1 in 1.8 million procedures, but contaminated endoscopes might cause outbreaks more often than other medical devices. Aim: The aim of this study was to describe the endoscope reprocessing procedure validated with microbiological cultures and ATP bioluminescence tool kits. Material and methods: We examined the endoscope reprocessing procedures at the Ministry of Health Bakirkoy Sadi Konuk Research Hospital using microbiological cultures and ATP bioluminescence tool kit during 2014. Results: We examined 42 flexible endoscopes and 4 (9%) endoscopes were found to be contaminated. Stenotrophomonas maltophilia (>100,000 cfu/mL) was isolated from irrigation bottles and extended-spectrum beta-lactamases and plasmid-mediated carbapenamase producing K. pneumonia and AmpC beta-lactamase producing P. aeruginosa (>100,000 cfu/mL) were isolated from elevator behind, respectively. Each step of the reprocessing procedure has been examined and revised with microbiological cultures and ATP bioluminescence tool kit. All endoscopes were found to be clean after revising the procedure. Conclusion: ATP-bioluminescence method provides shorter time to examine the endoscopes as a cost-effective method in the endoscope reprocessing. Microbiological monitoring and cleaning procedures of endoscopes should be defined in the healthcare settings with the frequencies. In case of any outbreak related to endoscopes, each step of procedure should be examined and corrected taking into guidelines, reported outbreaks, as well as instructions of endoscope manufacturers