The possibility of using mesenchymal stem cells for veterinary research and medicine Part 2 - Own examinations

SUMMARY In the first part of the two-part article series the authors introduced the possibility of the application of mesenchymal stem cell (MSC) in the field of human and principally veterinary medicine. In this second article they present their own results of the in vitro proliferative capacity and osteogenic differentiation potential of adipose tissue MSCs isolated from dogs of different ages. MSC cells were isolated from canine subcutaneous adipose tissue which were proliferated, characterized according to the international standard, differentiated and frozen for following experiments. Their observation revealed that MSCs from old and young donor Beagle dogs proliferated properly in vitro and they were able to show the required fibroblast- like morphology and differentiated into adipo-, chondro- and osteogenic lineages. The osteogenic differentiation of MSCs was followed by the measurement of alkaline phosphatase activity and calcium accumulation. Both of the determined parameters were higher in the case of younger donors compared to the old donor dog

99mTc-labelled nanosystem as tumour imaging agent for SPECT and SPECT/CT modalities

We report the synthesis, in vitro and in vivo investigation of folate-targeted, biocompatible, biodegradableself-assembled nanoparticles radiolabelled with 99mTc, as potential new SPECT or SPECT/CT imagingagent.Nanoparticles with hydrodynamic size in the range of 75?200 nm were prepared by self-assembly ofchitosan and folated poly

The possibility of using mesenchymal stem cells for veterinary research and medicine Part 1 - Literature review

SUMMARY In the first part of the two-part article series, based on the literature and their own research and experience, the authors introduce the stem cell types, detailed characteristics of mesenchymal stem cells, as well as the possibility of their veterinary uses and experimental results, particulary regarding the donor's age and the advantages and disadvantages of using allogene or autologue cell source for medical purposes. In the second part their own experimental results presented, the proliferative and the osteogenic differentiation capacity of adipose-derived mesenchymal stem cells from young and older dogs are demonstrated