Object tracking and matting for A class of dynamic image-based representations

Image-based rendering (IBR) is an emerging technology for photo-realistic rendering of scenes from a collection of densely sampled images and videos. Recently, an object-based approach for a class of dynamic image-based representations called plenoptic videos was proposed. This paper proposes an automatic object tracking approach using the level-set method. Our tracking method, which utilizes both local and global features of the image sequences instead of global features exploited in previous approach, can achieve better tracking results for objects, especially with non-uniform energy distribution. Due to possible segmentation errors around object boundaries, natural matting with Bayesian approach is also incorporated into our system. Furthermore, a MPEG-4 like object-based algorithm is developed for compressing the plenoptic videos, which consist of the alpha maps, depth maps and textures of the segmented image-based objects from different video plenoptic streams. Experimental results show that satisfactory renderings can be obtained by the proposed approaches. © 2005 IEEE.published_or_final_versio

An object-based approach to plenoptic videos

This paper proposes an object-based approach to plenoptic videos, where the plenoptic video sequences are segmented into image-based rendering (IBR) objects each with its image sequence, depth map and other relevant information such as shape information. This allows desirable functionalities such as scalability of contents, error resilience, and interactivity with individual IBR objects to be supported. A portable capturing system consisting of two linear camera arrays, each hosting 6 JVC video cameras, was developed to verify the proposed approach. Rendering and compression results of real-world scenes demonstrate the usefulness and good quality of the proposed approach. © 2005 IEEE.published_or_final_versio

An object-based approach to image/video-based synthesis and processing for 3-D and multiview televisions

This paper proposes an object-based approach to a class of dynamic image-based representations called "plenoptic videos," where the plenoptic video sequences are segmented into image-based rendering (IBR) objects each with its image sequence, depth map, and other relevant information such as shape and alpha information. This allows desirable functionalities such as scalability of contents, error resilience, and interactivity with individual IBR objects to be supported. Moreover, the rendering quality in scenes with large depth variations can also be improved considerably. A portable capturing system consisting of two linear camera arrays was developed to verify the proposed approach. An important step in the object-based approach is to segment the objects in video streams into layers or IBR objects. To reduce the time for segmenting plenoptic videos under the semiautomatic technique, a new object tracking method based on the level-set method is proposed. Due to possible segmentation errors around object boundaries, natural matting with Bayesian approach is also incorporated into our system. Furthermore, extensions of conventional image processing algorithms to these IBR objects are studied and illustrated with examples. Experimental results are given to illustrate the efficiency of the tracking, matting, rendering, and processing algorithms under the proposed object-based framework. © 2009 IEEE.published_or_final_versio

On the rendering and post-processing of simplified dynamic light fields with depth information

This paper studies the rendering and post-processing of a dynamic image-based representation called the simplified dynamic light fields (SDLF) (or plenoptic videos) with depth information. The user viewpoints are limited to a camera line to simplify the capturing and compression processes. By associating each image pixel with its depth value, methods for improving the rendering quality and detecting occlusions are proposed. Due to the limited sampling at depth discontinuities, adaptive lowpass filtering is applied to the detected occluded regions near object boundaries in order to suppress the aliasing artifacts. Rendering results using computer-generated images show that considerable improvement in rendering quality even for dynamic scenes with large depth variations.published_or_final_versio

The Plenoptic videos: Capturing, Rendering and Compression

This paper presents a system for capturing and rendering a dynamic image-based representation called the plenoptic videos. It is a simplified version of light fields for dynamic environment, where user viewpoints are constrained along the camera plane of a linear array of video cameras. The system consists of a camera array of 8 Sony CCX-Z11 CCD cameras and eight Pentium 41.8 GHz computers connected together through a 100 baseT LAN. Important issues such as multiple camera calibration, real-time compression, decompression and rendering are addressed. Experimental results demonstrated the usefulness of the proposed parallel processing based system in capturing and rendering high quality dynamic image-based representation using off-the-shelf equipment, and its potential applications in visualization and immersive television systems.published_or_final_versio

Relatório de estágio: Novadelta Comércio de Cafés España, SAU

O presente relatório enquadra-se no estágio curricular do Mestrado em Auditoria Empresarial e Pública ministrado pelo Instituto Superior de Contabilidade e Administração de Coimbra. O estágio teve lugar na empresa Novadelta Comercio de Cafés España, S.A.U, no Departamento de Contabilidade, tendo decorrido entre 2 de maio e 30 de outubro de 2012. As atividades do estágio visaram fundamentalmente percecionar de que forma a contabilidade é executada nesta organização, em particular no que respeita aos processos que envolvem pagamentos e recebimentos, bem como os procedimentos de controlo interno nas diversas áreas do negócio, mormente os relativos à informação financeira. Os procedimentos de controlo interno relativos à informação financeira (contabilidade) existentes nesta Empresa são de extrema importância, porque permitem que os utilizadores da informação financeira tenham maior confiança nas suas demonstrações financeiras. O controlo existente em cada área de atividade assume-se como um ponto forte para a Empresa, contribuindo para que o contacto direto com clientes, fornecedores e outras entidades seja assente na confiança, honestidade e amizade. Por outro lado, permite responder melhor à exigência de qualidade dos serviços. O estágio na Novadelta Espanha foi muito gratificante, na medida em que foi possível pôr em prática os conhecimentos teóricos adquiridos ao nível da contabilidade e controlo interno, bem como o desenvolvimento de novas competências, a autonomia no desenvolvimento das tarefas nessas áreas, adaptação a novos ambientes e fomentar o relacionamento interpessoal e intercultural

The Topology of Cosmological Reionization

Using the largest cosmological reionization simulation to-date (~24 billion particles), we use the genus curve to quantify the topology of neutral hydrogen distribution on scales > 1 Mpc as it evolves during cosmological reionization. We find that the reionization process proceeds primarily in an inside-out fashion, where higher density regions become ionized earlier than lower density regions. There are four distinct topological phases: (1) Pre-reionization at z ~ 15, when the genus curve is consistent with a Gaussian density distribution. (2) Pre-overlap at 10 < z < 15, during which the number of HII bubbles increases gradually with time, until percolation of HII bubbles starts to take effect, characterized by a very flat genus curve at high volume fractions. (3) Overlap at 8 < z < 10, when large HII bubbles rapidly merge, manifested by a precipitous drop in the amplitude of the genus curve. (4) Post-overlap at 6 < z < 8, when HII bubbles have mostly overlapped and the genus curve is consistent with a diminishing number of isolated neutral islands. After the end of reionization (z < 6), the genus of neutral hydrogen is consistent with Gaussian random phase, in agreement with observations.Comment: 9 pages, 6 figures, accepted by Ap

Author correction: High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia

In this Article, in the section entitled ‘Quantitative real-time PCR’ within the Supplementary Methods, the probe for the Bacteroides fragilis 16S real-time PCR reaction was listed incorrectly as 5ʹHEX-AGGGACTGGAAGGCTTTACTGCTTC-3ʹBHQ1. The correct probe for Bacteroides fragilis 16S should be listed as 5ʹHEX-ACACGTATCCAACCTGCCCTTTACTCG-3ʹBHQ1. The mistake was a result of a copy and paste error with a different primer set targeting a B. fragilis toxin gene. All qPCR reactions were performed using the correct probe, and therefore no data were affected

Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy

A comparative study of immature and mature bone marrow-derived dendritic cells (BMDCs) was first performed through an atomic force microscope (AFM) to clarify differences of their nanostructure and adhesion force. AFM images revealed that the immature BMDCs treated by granulocyte macrophage-colony stimulating factor plus IL-4 mainly appeared round with smooth surface, whereas the mature BMDCs induced by lipopolysaccharide displayed an irregular shape with numerous pseudopodia or lamellapodia and ruffles on the cell membrane besides becoming larger, flatter, and longer. AFM quantitative analysis further showed that the surface roughness of the mature BMDCs greatly increased and that the adhesion force of them was fourfold more than that of the immature BMDCs. The nano-features of the mature BMDCs were supported by a high level of IL-12 produced from the mature BMDCs and high expression of MHC-II on the surface of them. These findings provide a new insight into the nanostructure of the immature and mature BMDCs

Regulation of HSP27 on NF-κB pathway activation may be involved in metastatic hepatocellular carcinoma cells apoptosis

<p>Abstract</p> <p>Background</p> <p>During the process of metastasis, cells are subjected to various apoptotic stimuli. Aberrant expression of apoptotic regulators often contribute to cell metastasis. Heat shock protein 27(HSP27) is confirmed as an apoptosis regulator, but its antiapoptotic mechanism in metastatic hepatocellular carcinoma (HCC) cells remains unclear.</p> <p>Methods</p> <p>Levels of HSP27 protein and its phosphorylation in Hep3B, MHCC97L to MHCC97H cells with different metastatic potentials were determined by western blot analysis. MHCC97H cells were transfected with specific small interference RNA (siRNA) against HSP27. The <it>in vitro </it>migration and invasion potentials of cells were evaluated by Transwell assay. The apoptosis ratio of MHCC97H cells was analyzed by TUNEL staining and Flow Cytometry. Alteration of signal transduction pathway after HSP27 knockdown in MHCC97H cells was evaluated through a Human Q Series Signal Transduction in Cancer Gene Array analysis. Nuclear NF-κB contentration and endogenous IKK activity were demonstrated by ELISA assay. The association of IKKα, IKKβ, IκBα with HSP27 and the association between IKKβ and IKKα in MHCC97H cells were determined by co-immunoprecipitation assay followed by western blot analysis.</p> <p>Results</p> <p>HSP27 protein and its phosphorylation increased in parallel with enhanced metastatic potentials of HCC cells. siRNA-mediated HSP27 knockdown in MHCC97H significantly suppressed cells migration and invasion <it>in vitro </it>and induced cell apoptosis; the prominently altered signal transduction pathway was NF-κB pathway after HSP27 knockdown in MHCC97H cells. Furthermore, inhibition of HSP27 expression led to a significant decrease of nuclear NF-κB contentration and endogenous IKK activity. In addition, HSP27 was associated with IKKα, IKKβ, IκBα in three HCC cells above. ELISA assay and western blot analysis also showed a decrease of the association between IKKβ and IKKα, the association between phosphor-HSP27 and IKK complex, and an increase of total IκBα but reducing tendency of phosphor-IκBα when HSP27 expression was efficiently knocked down in MHCC97H cells.</p> <p>Conclusion</p> <p>Altogether, these findings revealed a possible effect of HSP27 on apoptosis in metastatic HCC cells, in which HSP27 may regulate NF-kB pathway activation.</p