EGFR-Mediated Carcinoma Cell Metastasis Mediated by Integrin αvβ5 Depends on Activation of c-Src and Cleavage of MUC1

Receptor tyrosine kinases and integrins play an essential role in tumor cell invasion and metastasis. We previously showed that EGF and other growth factors induce human carcinoma cell invasion and metastasis mediated by integrin αvβ5 that is prevented by Src blockade [1]. MUC1, a transmembrane glycoprotein, is expressed in most epithelial tumors as a heterodimer consisting of an extracellular and a transmembrane subunit. The MUC1 cytoplasmic domain of the transmembrane subunit (MUC1.CD) translocates to the nucleus where it promotes the transcription of a metastatic gene signature associated with epithelial to mesenchymal transition. Here, we demonstrate a requirement for MUC1 in carcinoma cell metastasis dependent on EGFR and Src without affecting primary tumor growth. EGF stimulates Src-dependent MUC1 cleavage and nuclear localization leading to the expression of genes linked to metastasis. Moreover, expression of MUC1.CD results in its nuclear localization and is sufficient for transcription of the metastatic gene signature and tumor cell metastasis. These results demonstrate that EGFR and Src activity contribute to carcinoma cell invasion and metastasis mediated by integrin αvβ5 in part by promoting proteolytic cleavage of MUC1 and highlight the ability of MUC1.CD to promote metastasis in a context-dependent manner. Our findings may have implications for the use and future design of targeted therapies in cancers known to express EGFR, Src, or MUC1

The Drosophila melanogaster host model

The deleterious and sometimes fatal outcomes of bacterial infectious diseases are the net result of the interactions between the pathogen and the host, and the genetically tractable fruit fly, Drosophila melanogaster, has emerged as a valuable tool for modeling the pathogen–host interactions of a wide variety of bacteria. These studies have revealed that there is a remarkable conservation of bacterial pathogenesis and host defence mechanisms between higher host organisms and Drosophila. This review presents an in-depth discussion of the Drosophila immune response, the Drosophila killing model, and the use of the model to examine bacterial–host interactions. The recent introduction of the Drosophila model into the oral microbiology field is discussed, specifically the use of the model to examine Porphyromonas gingivalis–host interactions, and finally the potential uses of this powerful model system to further elucidate oral bacterial-host interactions are addressed

The mass decontamination paradigm: Response relating to gas phase exposures and skin decontamination

This chapter describes the nature of gas exposure and the implications for decontamination of exposed persons in hazardous materials incidents. An approach for systematic investigation of gas-skin interaction is outlined and findings presented for ten common substances. The limitations and applicability of this approach for first responder advice are also described.Christina Baxter, Sharyn Gaskin, Michael Logan, and Dino Pisaniell

Einstellung von PI-Reglern bei Send-on-Delta-Abtastung: Regelkreisoptimierung unter Berücksichtigung von Energieeffizienz, Netzlast und Regelgüte

Energieeffizienz hat in Forschung und Alltag eine zentrale Bedeutung. Arbeiten verschiedene elektronische Geräte zusammen, um gemeinsam eine Regelungsaufgabe zu lösen, müssen sie miteinander kommunizieren. Ein Beispiel aus dem Alltag sind Funk-Raumtemperaturregler, bei denen ein batteriebetriebener Temperatursensor und ein Heizungsaktor (Stellantrieb am Heizungsventil) über drahtlose Kommunikation zusammenarbeiten. Diese Kommunikation benötigt oft mehr Energie als der Betrieb der eigentlichen (elektronischen) Funktionalität der Teilsysteme. Energieeffizienter als die in Regelkreisen übliche periodische (äquidistante) Abtastung ist – durch eine Verringerung der Nachrichtenrate – eine ereignisbasierte Abtastung. Send-on-Delta-Abtastung ist die am weitesten verbreitete Art der ereignisbasierten Abtastung. Dabei wird der Wert der Regelgröße (im Beispiel die Raumtemperatur) nicht in konstanten Zeitintervallen übertragen, sondern nur dann, wenn er sich um einen bestimmten Betrag geändert hat. Der mit einem Anteil von über 90 % im Praxiseinsatz am weitesten verbreitete Reglertyp ist der PID-Regler, wobei die meisten als „PID-Regler“ bezeichneten Regler aus verschiedenen Gründen keinen D-Anteil (Differential-Anteil) verwenden und daher als „PI-Regler“ bezeichnet werden können. Die vorliegende Arbeit verfolgt das Ziel, systematisch zu untersuchen, wie man PI-Regler einstellen sollte, um neben dem Erreichen einer hohen Regelgüte auch die Vorteile der Send-on-Delta-Abtastung bezüglich der Netzlastreduktion und Energieeffizienz bestmöglich auszunutzen. Die „Gewichtung“ dieser sich teilweise widersprechenden Kriterien ist anwendungsspezifisch einstellbar.Energy efficiency is very important both in science and everyday life. If different electronic devices work together, for example for solving a control task together, they have to communicate with each other. An everyday life example are room temperature controllers using radio communication between a battery-powered temperature sensor and a heating actuator. This communication often needs more energy than the operation of the actual (electronic) functionality of the components. More energy-efficient than the commonly used periodic sampling is event-based sampling, due to the reduction of the message rate. Send-on-delta sampling is the most widely-known kind of event-based sampling. In that case, the value of the controlled variable (e.g. the room temperature) is not transmitted equidistantly but only when it has changed by a specific amount. The most successful controller in practice is the PID controller. The most so-called “PID controllers” do not use the D part (differential action) for several reasons and can therefore be called “PI controllers”. This work analyses systematically how the parameters of a PI controller should be tuned to reach besides a high control quality also a good exploitation of the advantages of send-on-delta sampling regarding network load reduction and energy efficiency. The “weighting” of these partially contradicting criteria is application specifically adjustable